Genetic diversity and population structure of blast resistance genes in Thai upland rice germplasm

Sooklim, Chayaphathra; Chomnunti, Putarak; Jantasuriyarat, Chatchawan; Chukeatirote, Ekachai; Nilthong, Rungrote; Nilthong, Somrudee

doi:10.1007/s10658-022-02499-6

Cited by 5 publications

(6 citation statements)

References 42 publications

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“…Landraces are evolved from their wild progenitor through natural and arti cial selection and have maintained a high degree of genetic diversity (Zhang et al 2009), which are source of traits leading tolerance to various stresses (Sooklim et al 2022), as well as, developed robust rice varieties will result in greater yields within the available land, thereby might help in meeting the world's increased caloric demand (Khush 2005). Meanwhile, the identi cation of traits associated with yield potential and sustainability at target agro-ecological conditions is important prerequisite of successful selection strategy.…”

Section: Introductionmentioning

confidence: 99%

Genetic variation, population structure, and marker-trait association of rice (Oryza sativa L.) cultivars using morphological characteristics and molecular markers

Fatamatuzzohora

Hossain

Kang

et al. 2023

Preprint

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Indigenous germplasms are the excellent sources of diversity, however, they are prone to genetic erosion, therefore exploration of genetic variability in indigenous germplasm is an essential step for future breeding programs. Discovery of genetic mechanisms of the characters in landraces that could cope adverse situations is important for maintaining world-food supply. Here rice landraces were assessed for their genetic diversity and relatedness using morpho-physiological data and SSR markers and found significant variation among yield attributing features. Phenotypic and genotypic coefficients of variability were higher for filled grains, whereas high heritability was found for plant height. Panicle number and 100-grain weight displayed significant relationships with yield. Phylogenetic clustering of the agro-morphological traits divided the germplasms into 8 clusters, whereas SSR marker yielded 10 clusters. Markers produced 138 alleles varying from 2 to 9 with 77.53% polymorphic allele having 3.69 alleles per locus (average). The polymorphic information content indices and observed number of alleles varied among markers with 0.649 (average), exhibiting RM336, RM316, and RM287 have the greatest potentiality in exploring genetic diversity among genotypes. The genotypes Dolkochu, Jolduba, BRRI Dhan-32, and Mokbul were present in a single cluster and showed wider variability, indicating their potentiality to be parents in hybridization programs in crop breeding. Population structure study demonstrated that the genotypes were structurally diverse that grouped the accessions into two sub-populations (K = 2). Twenty-one marker-trait associations (p < 0.05) for 8 characteristics were identified. These diverse germplasms and polymorphic trait-linked markers might be suitable for molecular breeding.

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Section: Introductionmentioning

confidence: 99%

Genetic variation, population structure, and marker-trait association of rice (Oryza sativa L.) cultivars using morphological characteristics and molecular markers

Fatamatuzzohora

Hossain

Kang

et al. 2023

Preprint

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“…Numerous PCR-based markers have previously been developed and are being utilised to mine different germplasm panels that hold untapped resources of distinct alleles for blast resistance genes (Yadav et al, 2019). Various researchers have been screened large germplasm sets using blast gene specific markers (Sooklim et al, 2022;Jeevan et al, 2023;Panja et al, 2023). Thus the present study aimed to identify the landraces possessing targeted blast resistant genes (Pi5, Pi54, Pi9 and Pi2) using gene specific markers.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Traditional Rice Varieties for Leaf Blast Resistant Genes Pi5, Pi54, Pi9 and Pi2 using Gene Specific Markers

Shanmugam,

Suresh,

Ramanathan

et al. 2023

Res. Bio.

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Magnaporthe oryzae poses a serious risk to rice growing regions worldwide. To combat this, future breeding efforts that aim to develop resistant varieties will need to identify and screen blast-resistant cultivars from existing germplasms. Thus, present study aimed to identify four major blast resistant genes (Pi54, Pi5, Pi2 and Pi9) in 20 traditional rice varieties using functional and linked markers. Results of the present study identified that fifteen traditional rice landraces were found to possess at least one resistant gene and three traditional landraces (Aanaikomban, Chenellu and Jai Sri Ram) had two resistant genes. These identified traditional rice landraces could be used as promising donor against rice blast disease for future rice breeding programmes to develop superior cultivars.

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“…Notwithstanding, the pathogenic Pyricularia is highly divergent in genetic patterns [6], causing the rapid outbreak and transmission in rice cultivation areas to other adjacent areas. To prevent outbreak and transmission of pathogenic Pyricularia in rice for long-term control of RBD, the rice varieties with RBD resistance would be an efective strategy for longterm control of this disease [7].…”

Section: Introductionmentioning

confidence: 99%

“…In Tailand, landrace rice is the most important resource of genetic diversity containing responsive genes to abiotic and biotic stress [7,19]. More recently, rice blast resistance genes were characterized in landrace rice from various locations such as Bangladesh, China, Japan, Malaysia, Tailand, and Vietnam.…”

Section: Introductionmentioning

confidence: 99%

“…More recently, rice blast resistance genes were characterized in landrace rice from various locations such as Bangladesh, China, Japan, Malaysia, Tailand, and Vietnam. From these, more than 30 R genes were identifed in Asian landrace rice, i.e., Pi-36, Pi9, Pib, Pigm(t), Pik-p, Pik-h, Pi-ta, Piz [7,[20][21][22][23]. Of these 30 R genes, the Pi9, Pib, and Pi-ta were commonly found in resistance rice cultivars grown in Malaysia [20] and Tailand [7].…”

Section: Introductionmentioning

confidence: 99%

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Investigating Rice Blast Resistance Gene Distribution among Landrace Rice Varieties in Lower Northern Thailand for Improving Rice Cultivars

Urtgam

Sujipuli

Suyasunanont

et al. 2023

International Journal of Agronomy

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Rice blast disease caused by the fungus Pyricularia oryzae is considered as one of the severe diseases, leading to reduce tremendous rice productivity in its cultivated areas of Thailand. Due to the rapid evolution and high genetic diversity of the pathogen, the innate rice resistance (R) genes associated with defense mechanisms are significantly considered as the most important for rice breeding program to create new rice varieties, resistant to blast disease. This study aimed to investigate the rice blast R genes (Pi9, Pib, and Pi-ta) in 98 landrace rice germplasms collected from three different provinces in lower northern Thailand, Phichit (PCT), Phitsanulok (PLK), and Sukhothai (STI) through PCR assay. The results showed that the Pi-ta gene was presented in 29 different varieties, making it the most widespread, whereas the Pi9 and Pib genes were found in 28 and 25 varieties, respectively. The distribution percentage of studied genes in PLK and STI germplasms is higher than in PCT germplasms. Interestingly, only eight landrace rice varieties (varieties no. 46, 47, 48, 51, 66, 76, 81, and 90) collected from PLK and STI germplasms contain all of these three resistance genes. This finding provided the genetic information and diversity of the R genes across landrace rice varieties in the lower north of Thailand. Moreover, these R genes could be useful as genetic resources for rice improvement with resistance to blast disease through breeding program in the future.

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Genetic diversity and population structure of blast resistance genes in Thai upland rice germplasm

Cited by 5 publications

References 42 publications

Genetic variation, population structure, and marker-trait association of rice (Oryza sativa L.) cultivars using morphological characteristics and molecular markers

Genetic variation, population structure, and marker-trait association of rice (Oryza sativa L.) cultivars using morphological characteristics and molecular markers

Characterization of Traditional Rice Varieties for Leaf Blast Resistant Genes Pi5, Pi54, Pi9 and Pi2 using Gene Specific Markers

Investigating Rice Blast Resistance Gene Distribution among Landrace Rice Varieties in Lower Northern Thailand for Improving Rice Cultivars

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