Genetic Diversity and Relationship of Ogye Population in Korea Using 25 Microsatellite Markers

Abstract

The aim of this study was to evaluate the genetic diversity and relationships of Ogye populations in Korea. A total of 243 genomic DNA samples from 6 Ogye population (Yeonsan Ogye; YSO, Animal Genetic Resources Research Center Ogye; ARO, Chungbuk Ogye; CBO, Chungnam Ogye; CNO, Gyeongbuk Ogye; GBO, Seoul National University Ogye; SUO) and 3 introduced chicken breeds (Rhode Island Red; RIR, White Leghorn; LG, Cornish; CN) were used. Sizes of 25 microsatellite markers were decided using GeneMapper Software(v 5.0) after analyzing ABI 3130XL. A total of 153 alleles were observed and the range was 2 to 10 per each locus. The mean of expected and observed heterozygosity and PIC (Polymorphism Information Content) value was 0.53, 0.50, 0.46 respectively. The lowest genetic distance (0.073) was observed between YSO and SUO, and the highest distance (0.937) between the RIR and CBO. The results of clustering analysis suggested 3 clusters (ΔK=7.96). Excluding GBO population, 5 Ogye populations (YSO, ARO, CBO, CNO, SUO) were grouped in same cluster with high genetic uniformity (0.990, 0.979, 0.989, 0.994, 0.985 respectively). But GBO population was grouped in cluster 1 with low genetic uniformity (0.340). The results of this study can be use to basic data for the genetic evaluation and management of Ogye populations in Korea.

Population structure analysis of Yeonsan Ogye using microsatellite markers

“…The markers selected in this study had higher Ho and He values than those identified in a previous study that have used 25 MS markers [12]. However, these results may have caused by the relatively large number of samples used (509 samples), compared with the previous study (27 samples).…”

“…Fortunately, 20-30 years ago, when the restoration project of Korean native chicken was being carried out, some groups of YO were delivered to Seoul National University and the National Institute of Animal Science in Chungbuk, Chungnam, and Gyeongbuk to maintain the purebred population. According to Roh et al [12], each of these Ogye populations has the same phenotypic characteristics but different genetic diversity due to different population size and breeding strategies. Whereas the Gyeongbuk population is different from the other three populations because this population might be maintained as a crossbred despite their similar phenotypic characteristics.…”