Genetic diversity and relationships among Hypericum L. species by ISSR Markers: A high value medicinal plant from Northern of Iran

Abstract

Hypericum L. species are generally known locally in Iran with the names “Hofariqun” which Ebn Sina (or Bo Ali Sina) called it. Plants of the genus Hypericum have traditionally been used as medicinal plants in various parts of the world. Hypericum perforatum L. is the source to one of the most manufactured and used herbal preparations in recent years, especially as a mild antidepressant. Therefore, due to the importance of these plant species, we performed a molecular data for this species. For this study, we used 175 randomly collected plants from 17 species in 9 provinces. Amplification of genomic DNA using 10 primers produced 141 bands, of which 127 were polymorphic (95.78%). The obtained high average PIC and MI values revealed high capacity of ISSR primers to detect polymorphic loci among Hypericum species. The genetic similarities of 17 collections were estimated from 0.617 to 0.911. According to Inter-Simple sequence repeats (ISSR) markers analysis, H. androsaemum and H. hirtellum had the lowest similarity and the species of H. perforaturm and H. triquetrifolium had the highest similarity. The aims of present study are: 1) can ISSR markers identify Hypericum species, 2) what is the genetic structure of these taxa in Iran, and 3) to investigate the species inter-relationship? The present study revealed that ISSR markers can identify the species.

The Intensity of Agriculture in the Covid-19 from Indonesia – A Systematic Literature Review

“…In confirmation of the adverse effects of stressful conditions on germination indices, Yagmur and Kaydan [21] revealed that osmotic stress reduced germination traits of triticale seeds. The negative impacts of osmotic stress on the initial growth of seeds of different plants were also investigated and confirmed by other researchers [22][23][24]. Xu, et al [24] reported that drought stress and gibberellin priming significantly affected germination percentage, seedling length, dry weight of the treated seedlings, and seedling length and weight indices at p <0.01.…”

“…In general, since non-primed seeds spend time performing phases I and II (rapid water imbibition by seed and reactivation of metabolism, respectively) longer, primed seeds are one step ahead of non-primed seeds in terms of germination and emergence stages [32]. Therefore, the cultivation of primed seeds, especially in unfavorable environmental conditions, leads to increased germination rate and germination percentage [22]. Here, the highest germination rate was assigned to interactions of the absence of gibberellin×the absence of osmotic stress×Clark cultivar, the absence of gibberellin×the absence of osmotic stress×Hobbit cultivar, gibberellin treatment×the absence of osmotic stress×Clark cultivar, and gibberellin treatment×the absence of osmotic stress×Hobbit cultivar.…”

Assessment of Genetic Variation of Genus Paracaryum (Boraginaceae) By Rapd Markers

“…Genetic diversity is non randomly distributed among different populations and is influenced by various factors such as geography, breeding systems, dispersal mechanisms, life span etc. Changes in environmental conditions often lead to variation in levels of genetic diversity among different populations, and under adverse circumstances, populations with low variability are generally considered less adapted (Ma, et al, 2021a;2021b;Peng et al, 2021). Most authors agree that genetic diversity is necessary to preserve the long-term evolutionary potential of a species (Ren et al, 2021).…”

Genetic relationships between populations of Aegilops tauschii Coss. (Poaceae) using SCoT molecular markers

“…Other excellent characteristics include their simplicity of use, high polymorphism, the use of universal primers, low cost and gene targeted markers. This technique has been successfully used to assess genetic diversity and structure (Collard and Mackill 2009;Ma et al, 2021;Peng et al, 2021;Si et al, 2021;Sun et al, 2021;Miao et al 2018;Zou et al, 2019;Wang et al 2020;Xiaolong et al, 2021;Hou et al, 2021), construct DNA fingerprints, identify QTLs, and analyze differential gene expression and screen stress tolerance genes. The present study is the first attempt to use SCoT markers to assess the level of genetic diversity of Aegilops tauschii which were collected from the wild populations.…”