Genetic diversity and relationships in jute (<i>Corchorus</i> spp.) revealed by SSR markers

Abstract

Characterization of sixteen jute genotypes, from Corchorus olitorius L. and Corchorus capsularis L. using jute specific SSR marker attained a high polymorphism value of 92.20%. A total of 171 different alleles were amplified by 27 primer pairs with a mean of 6.33 ± 2.04 alleles per locus. The genetic diversity was also relatively high (0.81 ± 0.06). The Un-weighted Pair-group Method with Arithmetic averages (UPGMA) cluster analysis of the 16 jute genotypes produced a dendogram, which was in concordance with known information. The study reinforces the utility of SSR primers for providing useful and high levels of markers for individual plant genotypes even with a narrow genetic base. Key words: Jute; Genetic diversity; SSR; Genotypes; Polymorphism DOI: 10.3329/bjb.v38i2.5140 Bangladesh J. Bot. 38(2): 153-161, 2009 (December)

Genetic Diversity in Corchorus olitorius Genotypes Using Jute SSRs

“…It is seen from the dendrogram that a few indigenous genotypes of Indian origin were deviated from cluster I (maximum grouped) and grouped into cluster II (20) and cluster III (3), which indicates similarity at genomic level with the other members of the cluster, which was represented by markers. Jute is self-pollinating and highly incompatible for inter-specific cross hybridization, which results in a narrow genetic base, however results of the current investigation showed good percent of polymorphism, similar results were reported in jute [31,32,39] and in many other plant species [30].…”

“…High genetic diversity (0.76 ± 0.15) as reported among C. olitorius genotypes [49], whereas a mean value of 0.81 ± 0.06 was reported using SSR in Corchorus sp. [39], which is higher in comparison to the results observed in the current study.…”

“…Based on the initial studies [44] using these SSR markers, ten highly polymorphic SSR (Table 2) markers were employed to study genetic diversity among 138 different jute samples. The number of alleles per locus generated by each marker varied from 2 to 3, with an average of 2.3 alleles per locus, which differed as 4.61 ± 1.92, 3.04 and 6.33 ± 2.04 alleles per locus [31,32,39], respectively. The difference in the average number of alleles may be due to the different genotypes utilized in the study.…”

“…Wild jute species have been classified [37] and cold-tolerant and cold-sensitive jute germplasms were characterized using inter simple sequence repeat marker [38]. Recently, the utility of studying genetic variability for different traits in jute genotypes using jute specific SSR markers has been reported [39,40]. A comprehensive analysis of genetic diversity of C. olitorius germplasm is inadequate.…”

Genetic linkage map construction for white jute (Corchorus capsularis L.) using SRAP, ISSR and RAPD markers

“…Haque et al (2008) constructed a linkage map including 40 polymorphic randomly amplified polymorphic DNA (RAPD) markers in dark jute (C. olitorius). Recently, several genetic linkage maps were constructed using simple sequence repeat (SSR) markers (Das et al 2012 because the number of publicly available SSR markers in dark jute (C. olitorius) is increasing (Roy et al 2006, Mir et al 2007, Keka et al 2008, Huq et al 2009, Ghosh et al 2010. Das et al (2012) constructed a linkage map comprising six LGs covering 784.3 cM using 36 polymorphic SSRs in an RIL (recombinant inbred line) population from a cross between two C. olitorius genotypes (JRO524 and PPO4).…”