Genetic diversity and relationships of diploid and tetraploid cottons revealed using AFLP

Abdalla, A; Reddy, O.U.K.; El-Zik, K. M.; Pepper, Alan E.

doi:10.1007/s001220051639

Cited by 138 publications

(115 citation statements)

References 28 publications

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“…In this study, low to moderate level of genetic similarity among Gossypium species has been estimated. This report is consistent with the findings of Abdalla et al (2001)-calculated relatively high estimates of genetic diversity among Gossypium species using AFLP marker system. Among tetraploid germplasm lowest genetic similarity of G. hirsutum ''punctatum'' (ancient cultivated race Lacape et al 2007) with the other tetraploid germplasm reveals the existence of unique/useful alleles in this species.…”

Section: Genetic Diversity and Phylogenetic Relationship In The Genussupporting

confidence: 92%

“…For evolutionary studies of cotton species, basic requirement is to workout their phylogeny (Khan et al 2000;Abdalla et al 2001;Paterson et al 2002) and to estimate the extent of genetic diversity (Khan et al 2000). For genetic diversity and phylogeny studies of cotton species various methods based on morphology, meiotic behavior, genetic and molecular techniques have been deployed.…”

Section: Genetic Diversity and Phylogenetic Relationship In The Genusmentioning

confidence: 99%

“…G. hirsutum is predominantly cultivated on *90 % of the total cotton area whereas; limited area is under cultivation of G. barbadense, known for producing high quality lint fiber (Abdalla et al 2001;Rahman et al 2012). Like other cultivated crop species, all the cultivated tetraploids and diploids have a narrow genetic base within their respective gene pool (Liu et al 2005;Rahman et al 2008Rahman et al , 2011Shaheen et al 2010) because of developing cultivars from few genotypes (Rahman et al 2002b(Rahman et al , 2005a.…”

Section: Introductionmentioning

confidence: 99%

“…It is, therefore, vital to assess the genetic diversity and phylogenetic relationship among the available cotton species as a mandatory requirement before utilizing the species in molecular breeding programs. Numerous methods including conventional and DNA-based conventional marker systems have been employed for estimating the extent of genetic diversity among the cotton species (Menzel 1954;Wendel 1994, 2000;Abdalla et al 2001;Gutierrez et al 2002;Guo et al 2006;Lacape et al 2007;Rahman et al 2009;Kalivas et al 2011). Earlier, RAPD markers were applied for working out the phylogeny of 31 cotton species (Khan et al 2000).…”

Section: Introductionmentioning

confidence: 99%

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Pros and cons of using genomic SSRs and EST-SSRs for resolving phylogeny of the genus Gossypium

2013

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The genus Gossypium is comprised of 50 diverse cotton species representing eight different genomes (A through G and K), however, phylogenetic relationship using various DNA marker types such as RAPD and SSRs was determined on limited number of cotton species. In this report, we have demonstrated the application of genomic SSRs (gSSRs) and EST-SSRs, and after combining both the data sets, for resolving the phylogenies of 36 cotton species including seven races. Out of the 100 primer pairs surveyed (50 for gSSRs and 50 for ESTSSRs), 75 produced scorable amplification products in all species. Out of these, 73 were found to be polymorphic and amplified 135 alleles ranging from 1 to 5 alleles per SSR marker (average 2.87 alleles per marker). The gSSRs amplified higher number of alleles (72) compared to the EST-SSRs (63). In total 22 highly informative SSRs with PIC values C0.5 were identified. Genomic SSRs containing di-while EST-SSRs containing tri-nucleotide repeats exhibited high polymorphism compared to the other nucleotide repeats containing gSSRs/EST-SSRs. Number of tandem repeats and polymorphism were positively correlated. Neither the type of chromosome nor the location of the SSRs showed association with the polymorphism. Gossypium herbaceum var. africanum (Watt) Hutch. ex and Ghose and Gossypium robinsonii F. Muell. were found the most genetically diverse, while among races of Gossypium hirsutum L. ''yucatanense'' and G. hirsutum ''punctatum'' were found genetically diverse. Of the three data sets, clustering analysis based on EST-SSRs and combined data sets, revealed parallel results reported in earlier studies. This study further confirmed that Gossypium darwinii Watt has close relationship with Gossypium barbadense L. Moreover, Gossypium raimondii Ulbr. and G. herbaceum/Gossypium arboreum L. are close living relatives of the ancestor allotetraploid species. Our studies suggest that for resolving phylogenetic relationship among the various plant species EST-SSRs could be a better choice. This information can be instrumental in transferring novel alleles or loci from the wild species into the cultivated cotton species which would set a stage for cultivating genetically diverse cultivars-a way to achieve sustainable cotton production in changing climate.

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Section: Genetic Diversity and Phylogenetic Relationship In The Genussupporting

confidence: 92%

Section: Genetic Diversity and Phylogenetic Relationship In The Genusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pros and cons of using genomic SSRs and EST-SSRs for resolving phylogeny of the genus Gossypium

2013

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“…Over the last two decades, multiple genomic tools have been utilized for exploring the cotton genome. Different types of DNA markers such as restriction fragment length polymorphism (RFLP) [39,40], randomly amplified polymorphic DNA (RAPD) [41][42][43][44][45][46][47][48], amplified fragment length polymorphism (AFLP) [27,49], simple sequence repeat (SSR) or microsatellites [37,50,51], single nucleotide polymorphism (SNPs) [52][53][54][55], physical maps, genetic maps, mapped genes and QTLs, microarrays, gene expression profiling, BAC and BIBAC libraries, QTL fine mapping, resistance gene analogs (RGA), genome sequencing, non-fiber and non-ovule EST development, gene expression profiling, and association studies for various traits have been extensively used for understanding the cotton genome. Finally, the genome sequence information of G. hirsutum L. and its progenitor species will considerably expedite the cotton genomic research toward identifying new genes conferring various traits of interest, and would also help in identifying DNA markers linked with traits which can be used in MAS.…”

Section: Genomic Studies In Cottonmentioning

confidence: 99%

Genetic Mapping in Cotton

Bardak¹,

Hayat²,

Erdoğan³

et al. 2018

Past, Present and Future Trends in Cotton Breeding

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The genus Gossypium provides natural fiber for textile industry worldwide. Genetic improvement in cotton for traits of interest is not up to mark due to scarcity of adequate information about fiber production and quality. Use of DNA markers for overcoming the issues of selection associated with complex traits is the ultimate choice which may lead to initiate breeding by design. Numerous marker-trait associations have been identified for economical traits using linkage analysis in cotton. Currently there is need for developing high-density genetic maps using next-generation sequencing approaches together with genome-wide association studies (GWAS). Efforts have been started in this direction and several QTLs including fiber quality, yield traits, plant architecture, stomatal conductance and verticillium wilt resistance were identified. This chapter narrates genetic diversity, QTL mapping, association mapping and QTLs related to fiber quality traits. The incorporation of various genomic approaches and previously described marker strategies will pave the way for increase in fiber production.

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Dwarf‐cashew resistance to whitefly (Aleurodicus cocois) linked to morphological and histochemical characteristics of leaves

Goiana

Dias-Pini

Muniz

et al. 2019

Pest Management Science

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BACKGROUND: The cashew whitefly (CW), Aleurodicus cocois, is an important pest of cashew in Brazil. The use of resistant plants may be an effective strategy for the control of this pest. In a preliminary assay, we found that dwarf-cashew clones show different levels of resistance to CW. Here, we hypothesized that such resistance is associated with morphological characteristics of cashew leaves and their content of phenolic compounds. RESULTS: We determined (i) the attractiveness and suitability for oviposition of five dwarf-cashew clones towards CW, (ii) the leaf morphology and chemistry of those clones, and (iii) the relationship between leaf characteristics and resistance to CW.In greenhouse multiple-choice assays, PRO143/7 and CCP76 showed, respectively, the lowest and highest counts of both CW adults and eggs. Scanning electron microscopy (SEM) analysis revealed that PRO143/7 and EMBRAPA51 have, respectively, the highest and lowest numbers of leaf glandular trichomes. We found a negative correlation between number of trichomes in the abaxial surface of cashew leaves and CW oviposition. In addition, confocal microscopy analysis and histochemical tests with ferrous sulfate indicated a higher accumulation of phenolic compounds in the resistant clone PRO143/7 relative to the other clones. Dwarf-cashew clones did not significantly differ based on the number of leaf epicuticular striations, and the thickness of both leaf lamina and the epidermal layer. CONCLUSION: The resistance of dwarf-cashew plants to CW is associated with an elevated number of trichomes and accumulation of high levels of phenolics in leaves. Additionally, the contribution of epicuticular striation density and thickness of leaf lamina/epidermal layer are insignificant.

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Genetic diversity and relationships of diploid and tetraploid cottons revealed using AFLP

Cited by 138 publications

References 28 publications

Pros and cons of using genomic SSRs and EST-SSRs for resolving phylogeny of the genus Gossypium

Pros and cons of using genomic SSRs and EST-SSRs for resolving phylogeny of the genus Gossypium

Genetic Mapping in Cotton

Dwarf‐cashew resistance to whitefly (Aleurodicus cocois) linked to morphological and histochemical characteristics of leaves

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