Genetic diversity and relationships of sweetpotato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat (ISSR) and restriction analysis of chloroplast DNA

Huang, Junfei; Sun, Mei

doi:10.1007/s001220051386

Cited by 186 publications

(122 citation statements)

References 26 publications

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“…The concentration of template (genomic DNA) could affect a lot in any PCR reaction (Huang and Sun, 2000). RAPD and ISSR were used in combination to reveal the genetic similarity between L. rohita, C. catla and their hybrids.…”

Section: Discussionmentioning

confidence: 99%

Genetic Variability in Labeo rohita, Catla catla and their Hybrid (Labeo rohita ♂ ? Catla catla ♀) Populations Employing Randomly Amplified Polymorphic (RAPD)-Inter Simple Sequence Repeat (ISSR) Assays

Naveed¹

2017

IJAB

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The present study was conducted to estimate genetic diversity of hybrid produced by two major carps (Labeo rohita and Catla catla) using DNA fingerprinting RAPD-ISSRs assay. A total of 65 RAPD-ISSR combinations were studied to check polymorphism among the accessions. 866 loci were amplified with an average of 13.32 loci per primer and 45.26% were found informative. Primers: DDA31-ISSR1 and DDA32-ISSR2 were most informative producing 12 polymorphic bands each. A similarity of 82% and 63% was exhibited by C. catla and their hybrids and L. rohita and their hybrids, respectively. Similarity analysis resulted 92% similarity among L. rohita parents' group and 84% similarity for their hybrids. Cluster analysis divided all accessions into two clusters: A and B; placing L. rohita in-group A, while B cluster was divided into two Sub groups B1 and B2 having C. catla and hybrids, respectively. The low level of genetic diversity, clearly signposted the alarming need of diverse parental populations for fish breeding programs and saving hybrid gene pool losses. It is the first report molecular program for the selection of major carps for breeding of fish in seed hatcheries.

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Section: Discussionmentioning

confidence: 99%

Genetic Variability in Labeo rohita, Catla catla and their Hybrid (Labeo rohita ♂ ? Catla catla ♀) Populations Employing Randomly Amplified Polymorphic (RAPD)-Inter Simple Sequence Repeat (ISSR) Assays

Naveed¹

2017

IJAB

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“…The influence of human activity and forest fragmentation may play a prominent role in creating this species's current endangered status. (Prevost & Wilkinson, 1998), 以及物种的分类系统学比较 (Huang & Sun, 2000;Joshi et al, 2000)等方面, 同时也成为构建遗传图谱的有力工具 (Kojima et al, 1998;Sankar & Moore, 2001 图1 8个思茅木姜子居群的取样分布图(居群代号同表1) Fig. 1 Map showing locations of the eight sampled Litsea szemaois populations.…”

mentioning

confidence: 99%

Genetic diversity of Litsea szemaois, an endangered species endemic to China, detected by inter-simple sequence repeat (ISSR)

Chen¹,

Ci²,

Li³

et al. 2006

Biodiversity Science

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“…Genomic DNA was extracted from frozen and dried leaves of plants grown in the field. The leaf tissue was ground to a fine powder and DNA was extracted using the improved CTAB method (Huang & Sun 2000). The AFLP analysis was performed as described by Vos et al (1995).…”

Section: Methodsmentioning

confidence: 99%

Ploidy level and molecular phylogenic relationship among novel Ipomoea interspecific hybrids

Cao¹,

Tang²,

Li³

et al. 2014

CZECH J GENET PLANT

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Cao Q.-H., Tang J., Li A., Gruneberg W., Huamani K., Ma D. (2014): Ploidy level and molecular phylogenic relationship among novel Ipomoea interspecific hybrids. Czech J. Genet. Plant Breed., 50: 32-38.Interspecific hybridization can be used to broaden the genetic base, generate novel species, postulate genetic relationships, and to introgress elite alien genes. However, interspecific hybridizations using wild parents outside the Ipomoea section Batatas are very difficult and have not been much studied. We used an improved hybridization technology to generate three novel interspecific hybrids by crossing Ipomoea batatas (L.) Lam. × I. hederacea Jacq., I. batatas (L.) Lam. × I. muricata (L.) Jacq., and I. batatas (L.) Lam. × I. lonchophylla J.M. Black. The ploidy level of the interspecific hybrids was determined by flow cytometry. The cross, I. batatas × I. hederacea, yielded the first artificial pentaploid Ipomoea hybrid ever. The other two hybrids, I. batatas × I. hederacea and I. batatas × I. muricata were tetraploid. The first two hybrids showed normal storage roots, a significant improvement in the storage roots of currently existing interspecific Ipomoea hybrids. AFLP (Amplified Fragment Length Polymorphism) molecular markers were used to explore the genetic relationship of these three novel interspecific hybrids with three other natural diploid, tetraploid, and hexaploid species of the Ipomoea section Batatas. Cluster analysis of AFLP bands showed that these three new interspecific hybrids were closely related to cultivated sweet potato (I. batatas/L./Lam.), which indicated that these novel hybrids can be used as an interspecific bridge to transfer alien genes from wild to cultivated species.

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Genetic diversity and relationships of sweetpotato and its wild relatives in Ipomoea series Batatas (Convolvulaceae) as revealed by inter-simple sequence repeat (ISSR) and restriction analysis of chloroplast DNA

Cited by 186 publications

References 26 publications

Genetic Variability in Labeo rohita, Catla catla and their Hybrid (Labeo rohita ♂ ? Catla catla ♀) Populations Employing Randomly Amplified Polymorphic (RAPD)-Inter Simple Sequence Repeat (ISSR) Assays

Genetic Variability in Labeo rohita, Catla catla and their Hybrid (Labeo rohita ♂ ? Catla catla ♀) Populations Employing Randomly Amplified Polymorphic (RAPD)-Inter Simple Sequence Repeat (ISSR) Assays

Genetic diversity of Litsea szemaois, an endangered species endemic to China, detected by inter-simple sequence repeat (ISSR)

Ploidy level and molecular phylogenic relationship among novel Ipomoea interspecific hybrids

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