Genetic diversity and structure of the threatened species Sinopodophyllum hexandrum (Royle) Ying

Abstract

ABSTRACT. Sinopodophyllum hexandrum is an important medicinal plant that has been listed as an endangered species, making the conservation of its genetic diversity a priority. Therefore, the genetic diversity and population structure of S. hexandrum was investigated through inter-simple sequence repeat analysis of eight natural populations. Eleven selected primers generated 141 discernible fragments. The percentage of polymorphic bands was 37.59% at the species level, and 7.66-24.32% at the population level. Genetic diversity of S. hexandrum was low within populations (average H E = 0.0366), but higher at the species level (H E = 0.0963). Clear structure and high genetic differentiation were detected between populations using unweighted pair groups mean arithmetic and principle coordinate analysis. Clustering approaches clustered the eight sampled populations into three major groups, and AMOVA confirmed there to be significant variation between populations (63.27%). Genetic differentiation may have arisen through limited gene flow (N m = 0.3317) in this species. Isolation by distance among populations was determined by comparing genetic distance versus geographical distance using the Mantel test. The results revealed no correlation between spatial pattern and geographic location. Given the low within-population genetic diversity, high differentiation among populations, and the increasing anthropogenic pressure on this species, in situ conservation measures, in addition to sampling and ex situ preservation, are recommended to preserve S. hexandrum populations and to retain their genetic diversity.

Mahonia vs. Berberis Unloaded: Generic Delimitation and Infrafamilial Classification of Berberidaceae Based on Plastid Phylogenomics

“…would likely further exacerbate their critical conservation status given the stereotypical impression of Berberis as agricultural weeds. Additionally, because both P. dubium ( Lee et al, 2018 ) and S. hexandrum ( Liu W. et al, 2016 ) are also rare and exploited for traditional medicines, recognizing and elevating these two distinct species to the generic rank also confers an effective conservation strategy.…”

“…Additionally, because both P. dubium (Lee et al, 2018) and S. hexandrum (Liu W. et al, 2016) are also rare and exploited for traditional medicines, recognizing and elevating these two distinct species to the generic rank also confers an effective conservation strategy.…”

Conservation implications of the genetic diversity of Gymnospermium microrrhynchum in Korea

“…Genetic variation provides the resources on which populations rely to survive against drastic environmental changes and evolutionary adaptations (Liu et al, 2016a). Species adapted to local environments, or isolated for long periods, have low levels of genetic variation and are easily affected by small environmental changes (Hamrick et al, 1992).…”

Caracterização e seleção de marcadores moleculares em Croton linearifolius Mull. Arg. como subsídio para estudos genéticos

“…A estratégia de caracterização e seleção de iniciadores como etapa preliminar em estudos genéticos, tem sido adotada em estudos com diferentes espécies, a exemplo da caracterização da diversidade genética em populações naturais de C. heliotropiifolius (Rocha et al, 2016), de Sinopodophyllum hexandrum (Liu et al, 2016) e de Maytenus truncata (Simplicio et al, 2015). Para Croton linearifolius são escassos os estudos genético moleculares e apenas caracterizações iniciais foram disponibilizadas por Rocha (2015) envolvendo marcadores RAPD e ISSR.…”