Genetic Diversity and Structure of Pea (Pisum sativum L.) Germplasm Based on Morphological and SSR Markers

Rana, Jai Chand; Rana, Maneet; Sharma, Vikas; Nag, Akshay; Chahota, Rakesh Kumar; Sharma, T. R.

doi:10.1007/s11105-016-1006-y

Cited by 27 publications

(32 citation statements)

References 41 publications

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“…The average observed heterozygosity value was 0.37 in our collection, and this was higher than Italian grass pea germplasm [46] and comparable to reported values of 0.39 and 0.40 in Ethiopian grass pea germplasms studied by Ponnaiah and coauthors [47] and Shiferaw and coauthors [21], respectively. The expected heterozygosity was greater than the observed heterozygosity which is similar to other results in grass pea [29], pear [48], apple [49] and pea [50]. The highest number of alleles and expected heterozygosity were observed with the EST-SSR marker in locus S6, which was characterized as the most informative fragment because high values for these parameters exhibit the power of an SSR marker for differentiating accessions [51].…”

Section: Discussionsupporting

confidence: 85%

Genotyping of Low β-ODAP Grass Pea (Lathyrus sativus L.) Germplasm with EST-SSR Markers

Arslan

Basak

Aksu

et al. 2020

Braz. arch. biol. technol.

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Grass pea (Lathyrus sativus L.) is an important protein source in arid regions as both human and animal food. Despite its significance, the use of grass pea is limited by the presence of β-N-oxalyl-L-a,bdiaminopropionic acid (β-ODAP) which can cause neurological disorders. Breeding studies in grass pea have therefore focused on developing high-yielding varieties with low β-ODAP content. However, the narrow range of genetic diversity and the restricted genomic tools in grass pea have slowed progress in such breeding. The present investigation was conducted to explore the genetic diversity of low β-ODAP germplasm consisting of 22 accessions with 31 EST-SSR markers. The molecular analyses revealed a total of 133 alleles ranging from 142 to 330 bp with a mean number of alleles per locus of 4.29. The mean polymorphic information content (PIC) value was calculated as 0.49, and the EST-SSRs in loci S5, S6 and S116 were of the most informative PICs. A dendrogram based on Nei's genetic distance matrix revealed that breeding lines were grouped in two main clusters. Genetic distances were higher between GP6/GP11, GP4/GP11 and GP5/GP8 accessions which could be further used in crop improvement studies for developing wider genetic diversity.

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Section: Discussionsupporting

confidence: 85%

Genotyping of Low β-ODAP Grass Pea (Lathyrus sativus L.) Germplasm with EST-SSR Markers

Arslan

Basak

Aksu

et al. 2020

Braz. arch. biol. technol.

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“…Jing et al (2010) studied the genetic diversity of 3020 Pisum accessions using RBIP markers, which separated the landraces, cultivars and wild Pisum accessions into distinct groups, and provided a framework for designing core collections. Genetic variation of pea accessions based on SSR markers has also been reported in other studies and the test accessions were clustered into distinct gene pools (Kumari et al, 2013; Jain et al, 2014; Rana et al, 2017; Wu et al, 2017).…”

Section: Introductionsupporting

confidence: 71%

“…Pea growth habit can be indeterminate or determinate, and cotyledon color can be yellow, green or red. Pea accessions also differ substantially in yield potential, ease of harvest, vine length, maturity, seed shape, seed size, and disease resistance (Ouafi et al, 2016; Rana et al, 2017). Thus, knowledge of the genetic diversity of pea accessions is of importance to select genetically diverse parents and to broaden the genetic basis of the cultivated peas.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Mapping for Agronomic and Seed Quality Traits of Field Pea (Pisum sativum L.)

et al. 2019

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Genome-wide association study (GWAS) was conducted to identify loci associated with agronomic (days to flowering, days to maturity, plant height, seed yield and seed weight), seed morphology (shape and dimpling), and seed quality (protein, starch, and fiber concentrations) traits of field pea (Pisum sativum L.). A collection of 135 pea accessions from 23 different breeding programs in Africa (Ethiopia), Asia (India), Australia, Europe (Belarus, Czech Republic, Denmark, France, Lithuania, Netherlands, Russia, Sweden, Ukraine and United Kingdom), and North America (Canada and USA), was used for the GWAS. The accessions were genotyped using genotyping-by-sequencing (GBS). After filtering for a minimum read depth of five, and minor allele frequency of 0.05, 16,877 high quality SNPs were selected to determine marker-trait associations (MTA). The LD decay (LD1/2max,90) across the chromosomes varied from 20 to 80 kb. Population structure analysis grouped the accessions into nine subpopulations. The accessions were evaluated in multi-year, multi-location trials in Olomouc (Czech Republic), Fargo, North Dakota (USA), and Rosthern and Sutherland, Saskatchewan (Canada) from 2013 to 2017. Each trait was phenotyped in at least five location-years. MTAs that were consistent across multiple trials were identified. Chr5LG3_566189651 and Chr5LG3_572899434 for plant height, Chr2LG1_409403647 for lodging resistance, Chr1LG6_57305683 and Chr1LG6_366513463 for grain yield, Chr1LG6_176606388, Chr2LG1_457185, Chr3LG5_234519042 and Chr7LG7_8229439 for seed starch concentration, and Chr3LG5_194530376 for seed protein concentration were identified from different locations and years. This research identified SNP markers associated with important traits in pea that have potential for marker-assisted selection towards rapid cultivar improvement.

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“…It is suggested that use of morphological traits is unavoidable for distinctness, uniformity, and stability (Roldán-Ruiz et al, 2001;Cupic et al, 2009). Researchers have utilized morphological variability in combination with molecular markers to precisely estimate characteristic diversity for drawing inferences in many crops, including pea (Smýkal et al, 2008a;Sharma et al, 2010;Rana et al, 2017). In this study, the T8 landrace was determined as a promising landrace in terms of hay weight, plant height, hay crude protein, and relative feed value for yield and quality compared to commercial cultivars.…”

Section: Discussionmentioning

confidence: 99%

Forage pea (Pisumsativum var. arvense L.) landraces reveal morphological andgenetic diversities

Demirkol¹,

Yılmaz²

2019

Turk J Bot

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The objective of this study was to assess morphological and genetic diversities of 48 forage pea landraces collected from different locations at different altitudes in Turkey. Morphological, quality, and yield features were determined for the landraces and three control cultivars in three subsequent years. Genetic diversities of the landraces and cultivars were also monitored using microsatellite (SSR) markers. Our results revealed that the features of landraces are significantly different. The hay weights and the relative feed values were found to be significantly affected by altitude, with the landraces generally showing significantly higher hay weight and relative feed values at lower altitudes (P < 0.05). At the genetic level, 32 SSR primers led to distinct placement of one of the samples into a different clade of the dendrogram, showing that it is genetically different from the other 47 samples. This genetically different landrace had the highest forage value, suggesting that it shows higher prime forage features than the cultivars and the other landraces. Moreover, altitude and generally flower color were found to be important factors affecting the genetics of the landraces, as the landraces having white flowers or collected at similar altitudes were clustered well in the dendrogram. The results of this study reveal that the morphological and genetic diversities of forage pea landraces collected from different locations at different altitudes show variations. Such information could be used to develop forage pea landraces with improved characters that can be used in hay management.

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Genetic Diversity and Structure of Pea (Pisum sativum L.) Germplasm Based on Morphological and SSR Markers

Cited by 27 publications

References 41 publications

Genotyping of Low β-ODAP Grass Pea (Lathyrus sativus L.) Germplasm with EST-SSR Markers

Genotyping of Low β-ODAP Grass Pea (Lathyrus sativus L.) Germplasm with EST-SSR Markers

Genome-Wide Association Mapping for Agronomic and Seed Quality Traits of Field Pea (Pisum sativum L.)

Forage pea (Pisumsativum var. arvense L.) landraces reveal morphological andgenetic diversities

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