Genetic Diversity and Structure of Dalmatian Pyrethrum (Tanacetum cinerariifolium Trevir. /Sch./ Bip., Asteraceae) within the Balkan Refugium

Abstract

Dalmatian pyrethrum (Tanacetum cinerariifolium Trevir. /Sch./ Bip.) is an outcrossing, perennial insecticidal plant, restricted to the eastern Adriatic coast (Mediterranean). Amplified fragment-length polymorphisms (AFLP) were used to investigate the genetic diversity and structure within and among 20 natural plant populations. The highest level of gene diversity, the number of private alleles and the frequency down-weighted marker values (DW) were found in northern Adriatic populations and gradually decreased towards the southern boundary of the species range. Genetic impoverishment of these southern populations is most likely the result of human-related activities. An analysis of molecular variance (AMOVA) indicated that most of the genetic diversity was attributed to differences among individuals within populations (85.78%), which are expected due to the outcrossing nature of the species. A Bayesian analysis of the population structure identified two dominant genetic clusters. A spatial analysis of the genetic diversity indicated that 5.6% of the genetic differentiation resulted from isolation by distance (IBD), while 12.3% of the genetic differentiation among populations followed the pattern of isolation by environmental distance (IBED). Knowledge of the genetic diversity patterns of the natural populations and the mechanism behind these patterns is required for the exploitation and possible conservation management of this endemic and economically important species.

Influence of Soil Traits on Polyphenols Level in Moltkia petraea (Tratt.) Griseb. (Boraginaceae)

“…1A). The population Ml showed a higher (Grdiša et al 2014), Campanula pyramidalis L. (Lakušić et al 2013), Edraianthus tenuifolius (Waldst. et Kit.)…”

Germination of Tanacetum Cinerariifolium Seeds on Different Soil Types

“…This genus comprises more than 150 species, including those previously regarded as belonging to the separate genus Pyrethrum Zinn [5,6,7]. T. cinerariifolium is indigenous to the eastern Adriatic coast, and is also found in Croatia, Bosna and Herzegovina, and Montenegro [5,8,9].…”

Production potential and economic viability of Croatian pyrethrum ecotypes

“…Several wild populations still can be found in Croatia distributed along the Dalmatian coast, and in South Istria (Premantura) (Grdiša et al, 2009). Indigenous Dalmatian populations, have been screened so far, for its chemical diversity (Grdiša et al, 2009), pyrethrins content (Ban et al, 2010), and genetic diversity (Grdiša et al, 2014).…”