Genetic diversity and virulence characteristics of biofilm-producing uropathogenic Escherichia coli

Qasemi, Ali; Rahimi, Forough; Katouli, Mohammad

doi:10.1007/s10123-021-00221-w

Cited by 7 publications

(6 citation statements)

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“…Although Congo red uptake was evaluated as an indicator of curli generation and the strains showed fixation at 37°C (data not shown), biofilm formation could not be visualized under the conditions indicated in this study. As previously described, other relevant factors in adhesion and colonization were partially absent in the strains UTI-1_774U, UTI-2_245U, and UTI-3_455U; we suggest that the absence of one or more fitness factors causes the inhibition of biofilm formation (Qasemi et al, 2021).…”

Section: Discussionsupporting

confidence: 81%

Comparative genomic analysis of uropathogenic Escherichia coli strains from women with recurrent urinary tract infection

Flores-Oropeza,

Ochoa,

Cruz-Córdova

et al. 2024

Front. Microbiol.

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IntroductionRecurrent urinary tract infections (RUTIs) caused by uropathogenic Escherichia coli are costly public health problems impacting patients’ quality of life.AimIn this work, a comparative genomics analysis of three clinical RUTI strains isolated from bladder biopsy specimens was performed.Materials and methodsOne hundred seventy-two whole genomes of urinary tract E. coli strains were selected from the NCBI database. The search for virulence factors, fitness genes, regions of interest, and genetic elements associated with resistance was manually carried out. The phenotypic characterization of antibiotic resistance, haemolysis, motility, and biofilm formation was performed. Moreover, adherence and invasion assays with human bladder HTB-5 cells, and transmission electron microscopy (TEM) were performed.ResultsThe UTI-1_774U and UTI-3_455U/ST1193 strains were associated with the extraintestinal pathotypes, and the UTI-2_245U/ST295 strain was associated with the intestinal pathotype, according to a phylogenetic analysis of 172 E. coli urinary strains. The three RUTI strains were of clinical, epidemiological, and zoonotic relevance. Several resistance genes were found within the plasmids of these strains, and a multidrug resistance phenotype was revealed. Other virulence genes associated with CFT073 were not identified in the three RUTI strains (genes for type 1 and P fimbriae, haemolysin hlyA, and sat toxin). Quantitative adherence analysis showed that UTI-1_774U was significantly (p < 0.0001) more adherent to human bladder HTB-5 cells. Quantitative invasion analysis showed that UTI-2_245U was significantly more invasive than the control strains. No haemolysis or biofilm activity was detected in the three RUTI strains. The TEM micrographs showed the presence of short and thin fimbriae only in the UTI-2_245U strain.ConclusionThe high variability and genetic diversity of the RUTI strains indicate that are a mosaic of virulence, resistance, and fitness genes that could promote recurrence in susceptible patients.

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Section: Discussionsupporting

confidence: 81%

Comparative genomic analysis of uropathogenic Escherichia coli strains from women with recurrent urinary tract infection

Flores-Oropeza,

Ochoa,

Cruz-Córdova

et al. 2024

Front. Microbiol.

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“…This could be in part related to mortality risk, as patients with higher risk are admitted to the ICU. Like all microcins, Microcin C exhibits antibacterial activity and results in increased survival of isolates producing Microcin C. Other studies found that cvaC was associated with biofilm-producing UPEC and is frequently more present in isolates from patients with prostatitis or pyelonephritis than in isolates from cystitis [ 16 , 17 , 18 ]. Indeed, cvaC was not found to be significantly different between blood isolates and control isolates here.…”

Section: Resultsmentioning

confidence: 99%

Virulence Factor Genes in Invasive Escherichia coli Are Associated with Clinical Outcomes and Disease Severity in Patients with Sepsis: A Prospective Observational Cohort Study

et al. 2023

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Background: Escherichia coli harbours virulence factors that facilitate the development of bloodstream infections. Studies determining virulence factors in clinical isolates often have limited access to clinical data and lack associations with patient outcome. The goal of this study was to correlate sepsis outcome and virulence factors of clinical E. coli isolates in a large cohort. Methods: Patients presenting at the emergency department whose blood cultures were positive for E. coli were prospectively included. Clinical and laboratory parameters were collected at admission. SOFA-score was calculated to determine disease severity. Patient outcomes were in-hospital mortality and ICU admission. Whole genome sequencing was performed for E. coli isolates and virulence genes were detected using the VirulenceFinder database. Results: In total, 103 E. coli blood isolates were sequenced. Isolates had six to 41 virulence genes present. One virulence gene, kpsMII_K23, a K1 capsule group 2 of E. coli type K23, was significantly more present in isolates of patients who died. kpsMII_K23 and cvaC (Microcin C) were significantly more frequent in isolates of patients who were admitted to the ICU. Fourteen virulence genes (mchB, mchC, papA_fsiA_F16, sat, senB, iucC, iutA, iha, sfaD, cnf1, focG, vat, cldB, and mcmA) significantly differed between patients with and without sepsis. Conclusions: Microcins, toxins, and fimbriae were associated with disease severity. Adhesins and iron uptake proteins seemed to be protective. Two genes were associated with worse clinical outcome. These findings contribute to a better understanding of host-pathogen interactions and could help identifying patients most at risk for a worse outcome.

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“…The ability to form biofilms in epithelial cells is related to their persistence, leading to the severity and recurrence of the disease. Curly pili is one of the most important components of biofilm extracellular matrix (Qasemi et al., 2021 ). Our results indicate that curli is a virulence gene widely present in pathogenic E. coli from cattle and sheep in Xinjiang.…”

Section: Discussionmentioning

confidence: 99%

Isolation, identification, molecular typing, and drug resistance of Escherichia coli from infected cattle and sheep in Xinjiang, China

Xue

et al. 2023

Veterinary Medicine & Sci

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Background: Escherichia coli infections are common in Xinjiang, a major region of cattle and sheep breeding in China. Therefore, strategies are required to control E. coli.The aim of this study was to investigate the phylogenetic groups, virulence genes, and antibiotic resistance characteristics of E. coli isolates. Methods:In this study, 116 tissue samples were collected from the organs of cattle and sheep that were suspected of having E. coli infections between 2015 and 2019. Bacteria in the samples were identified using a biochemical identification system and amplification of 16S rRNA, and the phylogenetic groupings of E. coli isolates were determined by multiplex polymerase chain reactions. In addition, PCR detection and analysis of virulence factors, antibiotic resistance genes, and drug-resistant phenotypes of E. coli isolates were performed.Results: A total of 116 pathogenic E. coli strains belonging to seven phylogenetic groups were isolated, with the majority of isolates in groups A and B1. Among the virulence genes, curli-encoding crl had the highest detection rate of 97.4%, followed by hemolysin-encoding hlyE with the detection rate of 94.82%. Antimicrobial susceptibility test results indicated that the isolates had the highest rates of resistance against streptomycin (81.9%). Conclusion:These characteristics complicate the prevention and treatment of E. colirelated diseases in Xinjiang.

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Genetic diversity and virulence characteristics of biofilm-producing uropathogenic Escherichia coli

Cited by 7 publications

References 50 publications

Comparative genomic analysis of uropathogenic Escherichia coli strains from women with recurrent urinary tract infection

Comparative genomic analysis of uropathogenic Escherichia coli strains from women with recurrent urinary tract infection

Virulence Factor Genes in Invasive Escherichia coli Are Associated with Clinical Outcomes and Disease Severity in Patients with Sepsis: A Prospective Observational Cohort Study

Isolation, identification, molecular typing, and drug resistance of Escherichia coli from infected cattle and sheep in Xinjiang, China

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