Genetic diversity and virulence gene determinants of antibiotic-resistant Salmonella isolated from preharvest turkey production sources

Nayak, Rajesh; Stewart, Tabitha; Wang, R.-F.; Lin, Jau-Hong; Cerniglia, Carl E.; Kenney, P. Brett

doi:10.1016/s0168-1605(03)00330-1

Cited by 92 publications

(66 citation statements)

References 27 publications

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“…showed identical results in different biochemical tests including Methyl-Red, Voges-Proskauer, and Indole test and similar type of biochemical reaction as reported by Menconi et al (2010) and Bryan (I965). In this study, colony characteristics of E. coli (Table 2) observed in NA, EMB and SS agar were similar to the findings of Nayak et al (2004) and Buxton and Fraser (1977). In Gram's staining, the morphology of the isolated bacteria exhibited Gram negative short rod arranged in single or paired and motile which was supported by several authors (Buxton and Fraser, 1977;Merchant and Packer, 1967).…”

Section: Antibiotic Sensitivity Pattern Of Salmonella Spp (N = 5)supporting

confidence: 90%

Isolation and identification of bacterial pathogens from cloacal swabs of turkeys and their antimicrobial sensitivity patterns

Roy

Hossain

Rumi

et al. 2018

Asian J. Med. Biol. Res

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The present study was carried for the isolation, identification of bacterial pathogens from cloacal swabs of turkeys during the period from January-June, 2016. The entire research work was conducted in the Department of Microbiology, Faculty of Veterinary and Animal Science, Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur. The study was performed with 48 cloacal swab samples. The cloacal swab samples were collected carefully from three different Turkey Farms randomly and transferred aseptically to the laboratory. On the basis of morphology, staining, cultural and biochemical characteristics it was found that among the isolates 25(52.08%) samples were positive E. coli, 10(20.83%) samples were positive for Salmonella spp., 9(18.76%) samples were positive for both E. coli and Salmonella spp. and 4(8.33%) samples shown no growth in subculture media. Antibiogram profiles indicate that E. coli isolated were 100% sensitive to Azithromycin, Kanamycin and Ciprofloxacin, 80% sensitive to Cefradine, Vancomycin and Levofloxacin, 60% sensitive to Cefotetan and Nitrofurantoin and 40% sensitive to Erythromycin. The isolates were 100% resistant to Cloxacillin and Cefixime. On the other hand, Salmonella spp. were 100% sensitive to Azithromycin, Kanamycin, Levofloxacin and Ciprofloxacin, 80% sensitive to Nitrofurantoin and Teicoplanin, 60% sensitive to Vancomycin, Erythromycin and Cefixime and 20% sensitive to Cefotetan. The isolates were 100% resistant to cefradine and cloxacillin. So, for E. coli Azithromycin, Kanamycin and Ciprofloxacin were more sensitive and for Salmonella spp. Azithromycin, Kanamycin, Ciprofloxacin and Levofloxacin were highly sensitive. Diversified bacterial species were present in cloacal swabs of Turkeys. However, E. coli, Salmonella spp. infection might make the birds vulnerable for easy access of infection. It could be concluded that E. coli and Salmonella spp. may pass through the feces to the environment. It causes a potential human health hazards and can cause illness.

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Section: Antibiotic Sensitivity Pattern Of Salmonella Spp (N = 5)supporting

confidence: 90%

Isolation and identification of bacterial pathogens from cloacal swabs of turkeys and their antimicrobial sensitivity patterns

Roy

Hossain

Rumi

et al. 2018

Asian J. Med. Biol. Res

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“…Numerous reports have documented the highly discriminatory nature of PFGE in successfully tracking infections of different Salmonella serotypes to certain geographic areas, including particular farms, and have subsequently linked human illness to exposure to animals or contaminated foods (4,16,17,28,36,42,48,49). However, a more discriminating method may be required to understand the evolutionary relatedness of epidemiologically unrelated populations (9,11,24,29,43,46).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Multilocus Sequence Typing, Pulsed-Field Gel Electrophoresis, and Antimicrobial Susceptibility Typing for Characterization of Salmonella enterica Serotype Newport Isolates

et al. 2006

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In the United States, multidrug-resistant phenotypes of Salmonella enterica serotype Newport (commonly referred to as MDR-AmpC) have emerged in animals and humans and have become a major public health problem. Although pulsed-field gel electrophoresis (PFGE) is the current "gold standard" typing method for Salmonella, multilocus sequence typing (MLST) may be more relevant to investigations exploring evolutionary and population biology relationships. In this study, 81 Salmonella enterica serotype Newport isolates from humans, food animals, and retail foods were examined for antimicrobial susceptibility and characterized using PFGE and MLST of seven genes, aroC, dnaN, hemD, hisD, purE, sucA, and thrA. Forty-nine percent of the isolates were resistant to nine or more of the tested antimicrobials. Salmonella isolates displayed resistance most often to sulfamethoxazole (57%), streptomycin (56%), tetracycline (56%), ampicillin (52%), and ceftiofur (49%) and, to a lesser extent, to kanamycin (19%), trimethoprim-sulfamethoxazole (17%), and gentamicin (11%). A total of 43 PFGE patterns were generated using XbaI, indicating a genetically diverse population. The largest PFGE cluster contained isolates from clinically ill swine, cattle, and humans. MLST resulted in 12 sequence types (STs), with one type encompassing 62% of the strains. Ten new sequence types and one novel allele type were identified. Furthermore, MLST typing showed that strains closely related by PFGE clustered in major STs, whereas more distantly related strains were separated into two clusters by PFGE. The results of this study demonstrated that the MLST scheme employed here clustered S. enterica serovar Newport isolates in distinct molecular populations, and strain discrimination was enhanced by combining PFGE, antimicrobial susceptibility, and MLST results.

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“…4 In addition, S. enterica serovar Heidelberg strains are found throughout the turkey production and processing environments, and in retail turkey products. [5][6][7] Because there has been a significant increase in per capita consumption of poultry products over the past 50 years, and because the majority of salmonellosis cases are associated with consumption of contaminated foods, 1,8 the contamination of poultry products with S. enterica serovar Heidelberg has become a major health concern. 9 Adding to this concern is the emergence and spread of antimicrobial resistant strains of S. enterica serovar Heidelberg 9,10 potentially caused by the use of antimicrobial agents in the animal production environment and is threatening the management of salmonellosis in both veterinary and human clinical practice.…”

Section: Susceptibility Testing Methodsmentioning

confidence: 99%

Comparison of Salmonella enterica serovar Heidelberg Susceptibility Testing Results

Nayak

Call²,

Kaldhone³

et al. 2007

Clinical Medicine & Research

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Objective: Disk diffusion and broth dilution assays are conventionally used for antimicrobial susceptibility testing (AST) of bacteria. The goal of this study was to determine the correlation of results from different AST methods for the Salmonella enterica serovar Heidelberg.Design: S. enterica serovar Heidelberg (n=105) strains were tested using 4 different AST methods: agar disk diffusion, broth microdilution using Sensititre with the NARMS (CMV1AGNF) panel, manual broth microdilution and Vitek with GNS-207 cards. Methods: AST was performed using standardized methods and Clinical and Laboratory StandardsInstitute recommended quality control organisms. Eight drugs were common to all testing methods including amikacin, amoxicillin/clavulanic acid, ampicillin, chloramphenicol, ciprofloxacin, gentamicin, tetracycline and trimethoprim/sulfamethoxazole. Results:No resistance to amikacin and ciprofloxacin was detected. Overall, the agreement of the AST results among all four methods for the drugs tested was: amikacin (100%), amoxicillin/clavulanic acid (96.1%), ampicillin (97.1%), chloramphenicol (96.2%), ciprofloxacin (100%), gentamicin (80.0%), tetracycline (80.0%) and trimethoprim/sulfamethoxazole (94.3%). There was 97.1%, 95.5% and 98.0% overall agreement between the reference diffusion method and the manual broth microdilution, Sensititre microdilution and Vitek methods, respectively. Conclusion:The study indicated that AST methods correlated with one another when testing S. enterica serovar Heidelberg isolates, with a few exceptions. In general, discrepancies among the methods were due to isolates being interpreted as intermediately susceptible or due to an increased number of resistances detected with Sensititre and a lower number with Vitek.

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Genetic diversity and virulence gene determinants of antibiotic-resistant Salmonella isolated from preharvest turkey production sources

Cited by 92 publications

References 27 publications

Isolation and identification of bacterial pathogens from cloacal swabs of turkeys and their antimicrobial sensitivity patterns

Isolation and identification of bacterial pathogens from cloacal swabs of turkeys and their antimicrobial sensitivity patterns

Comparison of Multilocus Sequence Typing, Pulsed-Field Gel Electrophoresis, and Antimicrobial Susceptibility Typing for Characterization of Salmonella enterica Serotype Newport Isolates

Comparison of Salmonella enterica serovar Heidelberg Susceptibility Testing Results

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