Genetic diversity and virulence of Colletotrichum lupini isolates collected in Chile

Riegel, Ricardo; Véliz, Débora; Baer, Ingrid Von; Quitral, Yerko; Muñoz, Manuel

doi:10.1590/s1982-56762010000300002

Cited by 11 publications

(10 citation statements)

References 29 publications

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“…No início do século 21 a antracnose generalizouse em termos geográficos, tornando-se relevante em praticamente todas as regiões do mundo onde se cultivam plantas do género Lupinus (Nirenberg et al, 2002;Thomas e Sweetingham, 2004;Lotter e Berger, 2005;Thomas et al, 2008;Semaškienė et al, 2008;Riegel et al, 2010;Falconí et al, 2013;Talhinhas et al, 2016). Em Portugal, a doença é extremamente comum nos Açores em L. albus e L. luteus (tremocilha), apesar de ter sido possível recolher testemunhos que demonstram uma origem recente para um tão elevado nível de incidência e severidade (Talhinhas, 2002).…”

Section: Introductionunclassified

Avaliação da resistência à antracnose em germoplasma de Lupinus spp.

Talhinhas¹,

Neves-Martins²,

Oliveira³

2016

Rev. Ciênc. Agr.

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R E S U M OUma das principais limitações à cultura de tremoceiros (pertencentes a várias espécies de Lupinus) em diversas partes do mundo resulta da sua suscetibilidade à antracnose, uma doença causada pelo fungo Colletotrichum lupini. Ao afetar o caule principal, esta doença pode causar a morte da planta, levando a perdas de produção frequentemente elevadas. A doença foi reportada apenas recentemente e a reduzida variabilidade genética do seu agente causal sugere uma origem recente do fungo. Pelo contrário, a grande riqueza genética encontrada no germoplasma de diversas espécies de Lupinus, algumas das quais tendo o território português como parte significativa da sua área de distribuição natural, impele estudos com vista à caracterização do grau de resistência a esta doença em acessos desse germoplasma. Após análise duma coleção de germoplasma contendo 51 acessos de Lupinus albus, 46 de L. angustifolius, 19 de L. luteus e 10 de L. mutabilis, através de inoculações em condições artificiais, foi identificado germoplasma de L. albus e de L. angustifolius com níveis promissores de resistência, respetivamente em variedades tradicionais e em germoplasma espontâneo português. O estudo do efeito da doença nos componentes da produção, em condições de campo, revelou que o número de vagens por infrutescência foi o componente da produção mais afetado, ilustrando um elevado aborto floral atribuído à doença. Estes resultados permitem uma utilização mais informada do germoplasma das espécies de Lupinus estudadas nos respetivos programas de melhoramento de plantas. Palavras-chave: tremoceiro; Lupinus; Colletotrichum lupini; Colletotrichum acutatum; antracnose. A B S T R A C TAnthracnose, caused by the fungus Colletotrichum lupini, is one of the main limitations to the cultivation of several lupin species in different parts of the world. Affecting the main stem, this disease may cause plant death, leading to frequently high yield losses. This disease was reported only recently and the narrow genetic diversity of its pathogen suggests a recent origin for this fungus. On the contrary, the vast genetic richness found in the germplasm of diverse Lupinus species, some of them having the Portuguese territory as important component of their natural distribution areas, call for the study targeting the characterisation of disease resistance in such germplasm accessions. The analysis of a germplasm collection comprising 51 Lupinus albus, 46 L. angustifolius, 19 L. luteus and 10 L. mutabilis accessions using artificial inoculation conditions pointed out L. albus and L. angustifolius accessions with promising levels of resistance, on Portuguese landraces and wild germplasm respectively. Under field conditions, the effect of the disease on yield components affected mostly the number of pods per pod set, suggesting a high flower abortion due to the disease. These results enable a more informed use of the germplasm of these Lupinus species on the respective breeding programmes.

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Section: Introductionunclassified

Avaliação da resistência à antracnose em germoplasma de Lupinus spp.

Talhinhas¹,

Neves-Martins²,

Oliveira³

2016

Rev. Ciênc. Agr.

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“…C. lupini is a major threat for lupin crops and control solutions need to be found in order to use this leguminous crop in rotations as a promising alternative for protein sources. A few studies have been performed to identify its taxonomy through phylogenetic analysis [ 74 , 75 , 76 , 77 , 78 ], describe its morphology [ 75 , 77 , 78 ] and symptoms in the field [ 4 ] or evaluate the effect of temperature on its in vitro and in planta growth [ 78 , 79 , 80 ]. In this study, Illumina RNA sequencing and nLC mass spectrometry were used to identify candidate genes and proteins associated with C. lupini pathogenicity over the course of infection in lupin plants.…”

Section: Discussionmentioning

confidence: 99%

Deciphering the Infectious Process of Colletotrichum lupini in Lupin through Transcriptomic and Proteomic Analysis

et al. 2020

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The fungal phytopathogen Colletotrichum lupini is responsible for lupin anthracnose, resulting in significant yield losses worldwide. The molecular mechanisms underlying this infectious process are yet to be elucidated. This study proposes to evaluate C. lupini gene expression and protein synthesis during lupin infection, using, respectively, an RNAseq-based transcriptomic approach and a mass spectrometry-based proteomic approach. Patterns of differentially-expressed genes in planta were evaluated from 24 to 84 hours post-inoculation, and compared to in vitro cultures. A total of 897 differentially-expressed genes were identified from C. lupini during interaction with white lupin, of which 520 genes were predicted to have a putative function, including carbohydrate active enzyme, effector, protease or transporter-encoding genes, commonly described as pathogenicity factors for other Colletotrichum species during plant infection, and 377 hypothetical proteins. Simultaneously, a total of 304 proteins produced during the interaction were identified and quantified by mass spectrometry. Taken together, the results highlight that the dynamics of symptoms, gene expression and protein synthesis shared similarities to those of hemibiotrophic pathogens. In addition, a few genes with unknown or poorly-described functions were found to be specifically associated with the early or late stages of infection, suggesting that they may be of importance for pathogenicity. This study, conducted for the first time on a species belonging to the Colletotrichum acutatum species complex, presents an opportunity to deepen functional analyses of the genes involved in the pathogenicity of Colletotrichum spp. during the onset of plant infection.

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“…This natural adaptation for nutrient acquisition, not present in most major crops, is highly relevant for a sustainable agriculture, helping to face the global challenge of food security, with lower fertilizer and water footprints. However, elite germplasm of L. luteus is susceptible to anthracnose disease in high rainfall areas, meaning its yield can be dramatically reduced 19,54,55 . Even more, fluctuating temperatures, frost, day length, and other climate changes, means flowering time is another important trait.…”

Section: Discussionmentioning

confidence: 99%

Genetic and comparative mapping of Lupinus luteus L. highlight syntenic regions with major orthologous genes controlling anthracnose resistance and flowering time

Lichtin

Salvo-Garrido

Till

et al. 2020

Sci Rep

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Anthracnose susceptibility and ill-adapted flowering time severely affect Lupinus luteus yield, which has high seed protein content, is excellent for sustainable agriculture, but requires genetic improvement to fulfil its potential. This study aimed to (1) develop a genetic map; (2) define collinearity and regions of synteny with Lupinus angustifolius; and (3) map QTLs/candidate genes for anthracnose resistant and flowering time. A few linkage groups/genomic regions tended to be associated with segregation distortion, but did not affect the map. The developed map showed collinearity, and syntenic regions with L. angustifolius. Major QTLs were mapped in syntenic regions. Alleles from the wild parent and cultivar, explained 75% of the phenotypic variance for anthracnose resistance and 83% for early flowering, respectively. Marker sequences flanking the QTLs showed high homology with the Lanr1 gene and Flowering-locus-T of L. angustifolius. This suggests orthologous genes for both traits in the L. luteus genome. The findings are remarkable, revealing the potential to combine early flowering/anthracnose resistant in fulfilling yield capacity in L. luteus, and can be a major strategy in the genetic improvement and usage of this species for sustainable protein production. Allele sequences and PCR-marker tagging of these genes are being applied in marker assisted selection.

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Genetic diversity and virulence of Colletotrichum lupini isolates collected in Chile

Cited by 11 publications

References 29 publications

Avaliação da resistência à antracnose em germoplasma de Lupinus spp.

Avaliação da resistência à antracnose em germoplasma de Lupinus spp.

Deciphering the Infectious Process of Colletotrichum lupini in Lupin through Transcriptomic and Proteomic Analysis

Genetic and comparative mapping of Lupinus luteus L. highlight syntenic regions with major orthologous genes controlling anthracnose resistance and flowering time

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