Genetic Diversity Assessment and Identification of New Sour Cherry Genotypes Using Intersimple Sequence Repeat Markers

Abstract

Iran is one of the chief origins of subgenus Cerasus germplasm. In this study, the genetic variation of new Iranian sour cherries (which had such superior growth characteristics and fruit quality as to be considered for the introduction of new cultivars) was investigated and identified using 23 intersimple sequence repeat (ISSR) markers. Results indicated a high level of polymorphism of the genotypes based on these markers. According to these results, primers tested in this study specially ISSR-4, ISSR-6, ISSR-13, ISSR-14, ISSR-16, and ISSR-19 produced good and various levels of amplifications which can be effectively used in genetic studies of the sour cherry. The genetic similarity among genotypes showed a high diversity among the genotypes. Cluster analysis separated improved cultivars from promising Iranian genotypes, and the PCoA supported the cluster analysis results. Since the Iranian genotypes were superior to the improved cultivars and were separated from them in most groups, these genotypes can be considered as distinct genotypes for further evaluations in the framework of breeding programs and new cultivar identification in cherries. Results also confirmed that ISSR is a reliable DNA marker that can be used for exact genetic studies and in sour cherry breeding programs.

Evaluation of Genetic Diversity by Morphological, Biochemical and Molecular Markers in Sour Cherry Genotypes Collected from the Kelkit Basin of Turkey

“…Similarity index in cherry showed a change from 0.36 to 0.91 between genotypes in the RAPD analysis [33]. In the ISSR marker system, a similarity index varying between 0.61 and 0.79 was formed [5]. The results of the molecular marker analysis of the current study are…”

“…Sour cherry is generally consumed fresh and used in the jam and marmalade industry as fruit juice and liquor production [5,6]. In addition, sour cherry is among the products bene cial to human health due to phytochemicals such as phenolic substances, antioxidants, avonoids, and anthocyanins [7].…”

“…Molecular marker studies such as RAPD [12], SSR [13], ISSR [5], morphological marker studies [14], studies in which biochemical content is determined has been and continues to be done to determine the genetic diversity in sour cherry [15]. As in most plants, factors such as yield, owering, disease, and drought resistance are controlled by many genes, and it is not reliable to determine them with a single method.…”

An overview of remote monitoring methods in biodiversity conservation

“…Prior sequence knowledge is not required for primer designing as repeat sequence is used to amplify these inter-microsatellite regions (Ng and Tan 2015 ). It overcomes all the limitations possessed by other markers such as RAPD and AFLP which are associated with low reproducibility (Najafzadeh et al 2014 ). Genetic diversity and population structure analysis have been performed among 11 populations of Bergenia ciliata using 15 ISSR markers.…”