Genetic diversity assessment in pea cultivars and lines using the SSR analysis

Гайнуллина, К. П.; Kuluev, B. R.; Давлетов, Ф. А.

doi:10.30901/2227-8834-2020-3-70-80

Cited by 8 publications

(8 citation statements)

References 13 publications

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“…It is one of the crops with a sufficiently high-altitude distribution limit in Russia -up to 2,000-2,480 meters above sea level. The wide area and zones of pea cultivation indicate its great ecological plasticity [13][14][15][16][17][18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED: Study of genetic polymorphism of grain pea cultivars

Gainullina,

Galikeeva,

Galimova

2023

E3S Web Conf.

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Pea (Pisum sativum L., 1753) is an essential pulse crop of global importance, which is a source of complete high-quality protein. Breeding new pea cultivars with improved economic valuable traits and high adaptive abilities is important for increasing the production of valuable high-protein pea grain. The key role in breeding is assigned to the initial material. A decrease in the genetic diversity of the initial material of cultivated plants, including pea, has become the main problem of modern breeding. In this regard, the issue of studying and preserving the genetic polymorphism of pea is relevant. Molecular genetic markers of simple sequence repeat (SSR) are widely used for this purpose. Here we aim to (1) to assess morphobiological and economical valuable traits and (2) analyze the molecular genetic diversity of the original pea material. The object of the research work was 20 grain pea cultivars. The yield structure elements of these cultivars were evaluated, and their molecular genetic polymorphism was analyzed using SSR markers AA5, AA355, AB28, AB71, and AD147. Early-ripening and highly productive cultivars were identified; they can be recommended for use as the initial material for pea breeding. As a result of the SSR analysis, 26 polymorphic alleles were identified, on average 5.2 alleles per locus. All studied cultivars were distinguished by a unique combination of alleles at five microsatellite loci, which allowed them to be identified. Based on the data of molecular genetic analysis, a cluster analysis was carried out, and a dendrogram was constructed. On the dendrogram, the studied cultivars were grouped according to the features of their morphological and economically valuable traits.

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Section: Introductionmentioning

confidence: 99%

RETRACTED: Study of genetic polymorphism of grain pea cultivars

Gainullina,

Galikeeva,

Galimova

2023

E3S Web Conf.

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“…Nasiri et al [33] found an average PIC value of 0.72 for 77 pea accessions from 17 different countries with 10 microsatellites. However, most studies found lower PIC values than our study, between 0.3 and 0.65, most using a larger number of microsatellites [14,[16][17][18][20][21][22][23][24]28,[30][31][32]. Singh et al [20] use 20 microsatellites to assess the genetic diversity of 47 pea accessions from India and obtained an average PIC value of 0.55, ranging from 0.04 to 0.85.…”

Section: Polymorphism Information Contentcontrasting

confidence: 57%

“…The genetic diversity measured as polymorphism information content (PIC) ranges from 0.15 to 0.96 for different types and numbers of microsatellites [15]. The microsatellite technique is also efficient when analyzing smaller dedicated pea collections such as garden peas, forage peas, landraces and historical collections from various countries, e.g., Tunisia, Anatolia, Australia, India, and from gene banks [16][17][18][19][20][21][22][23][24][25]. These studies included between 8 and 34 microsatellites showing PIC values ranging from 0.19 to 0.84.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Genetic Diversity and Protein Content of Scandinavian Peas (Pisum sativum)

Winther,

Rasmussen,

Poulsen

et al. 2023

Agronomy

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We produced homogeneous lines of 227 pea accessions from the Nordic Genetic Resource Center via single seed descent. The genetic diversity among these, mostly Scandinavian accessions, was investigated using three microsatellite markers, A9, AC58 and AA5. The microsatellites were highly informative and separated 153 of 194 accessions on a Neighbor Joining topology. The high polymorphism information content (PIC) values between 0.87 and 0.91 indicated that the gene bank material contains a large number of pea accessions with different breeding histories. The peas were grown in the field for two years and seed protein content showed variation between 9.3% and 34.1% over the years and accessions, respectively. The mean thousand seed weight was 152.05 g. More than 10 accessions had a protein content above 28%, showing that the collection has potential as breeding nursery for high-protein pea cultivars.

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“…Полученные данные могут свидетельствовать о взаимосвязи между мутациями в гене ABI3 и накоплением белка в семенах гороха. 1 , Candidate of Biological Sciences, researcher of the laboratory for plant genomics, rumyantsev-serg@mail.ru, ORCID ID: 0000-0002-5331-448Х; F. A. Davletov 3 , Doctor of Agricultural Sciences, head of the laboratory for primary legume breeding and seed production, davletovfa@mail.ru, ORCID ID: 0000-0002-7421-869Х; B. R. Kuluev 1 , Doctor of Biological Sciences, head of the laboratory for plant genomics, kuluev@bk.ru, ORCID ID: 0000-0002-1564-164Х The main source of vegetable protein in the world are leguminous crops, among which peas are the most common in Russia. Dozens and even hundreds of genes are involved in the regulation of biological processes associated with the maturation of seeds and the accumulation of reserve nutrients in them, including proteins.…”

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confidence: 99%

Analysis of nucleotide sequences of the ABI3 gene coding region in pea varieties with different percentage of seed storage proteins

Гайнуллина

Rumyantsev²,

Давлетов

et al. 2023

Zernovoe hozâjstvo Rossii

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The main source of vegetable protein in the world are leguminous crops, among which peas are the most common in Russia. Dozens and even hundreds of genes are involved in the regulation of biological processes associated with the maturation of seeds and the accumulation of reserve nutrients in them, including proteins. The study conducted on legume species related to peas indicate that one of the genes at the highest hierarchical level in this regulatory chain is the ABI3 transcription factor gene; however, its role in the biosynthesis and accumulation of seed storage proteins remains poorly studied. The purpose of the current work is to identify high-protein pea genotypes and analyze the nucleotide sequences of the coding region of the ABI3 gene to search for possible DNA polymorphisms associated with protein percentage in their seeds. The study was carried out in 2020–2021. The objects of the study were 37 pea varieties from the collection of genetic resources of leguminous crops of VIR. Protein percentage in seeds was determined by the Bradford method. Selection of primers and comparative analysis of nucleotide sequences were performed using the PrimerSelect and MegAlign programs. Sequencing was performed according to the Sanger method. There were identified pea varieties with the highest (‘Aksaisky Usaty 55’, ‘Sakharny’) and low (‘K-8361’ (VIR), ‘Fregat’) seed protein percentage. In these variety samples, the coding region of the ABI3 gene was sequenced. Comparative analysis of their nucleotide sequences with the sequence of the ABI3 gene fragment annotated in the GenBank database revealed significant variability among them: nucleotide substitutions, deletion of 6 nucleotides. In the high protein variety ‘Sakharny’, there has been found a 9-nucleotide insertion, leading to the insertion of three additional amino acids, and in the low-protein variety Fregat, there has been found a single nucleotide insertion, causing a shift in the reading frame. The data obtained may indicate a correlation between mutations in the ABI3 gene and protein accumulation in pea seeds.

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Genetic diversity assessment in pea cultivars and lines using the SSR analysis

Cited by 8 publications

References 13 publications

RETRACTED: Study of genetic polymorphism of grain pea cultivars

RETRACTED: Study of genetic polymorphism of grain pea cultivars

Assessment of Genetic Diversity and Protein Content of Scandinavian Peas (Pisum sativum)

Analysis of nucleotide sequences of the ABI3 gene coding region in pea varieties with different percentage of seed storage proteins

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