Genetic diversity assessment of a winter squash and pumpkin ( Cucurbita maxima Duchesne) germplasm collection based on genomic Cucurbita -conserved SSR markers

Kaźmińska, Karolina; Sobieszek, Krzysztof; Targońska-Karasek, Małgorzata; Korzeniewska, Aleksandra; Niemirowicz-Szczytt, K.; Bartoszewski, Grzegorz

doi:10.1016/j.scienta.2017.02.035

Cited by 33 publications

(33 citation statements)

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“…In terms of nuclear genetic variation, our results showed intermediate levels of allelic richness ( A = 2.96) compared with other domesticated Cucurbita species, based on different sets of nuclear microsatellite loci ( C. maxima : A = 4.3 [Kaźmińska et al., ]; C. pepo ssp. pepo : A = 3.43–3.8 [Gong et al., , ; Formisano et al., ; Enríquez et al., ; Castellanos‐Morales et al., ]; C. argyrosperma ssp.…”

Section: Discussionmentioning

confidence: 54%

“…sororia ( H E = 0.41 and H E = 0.43, respectively; Sánchez‐de la Vega et al., ), but slightly lower than values reported for other annual plants in microsatellite studies (i.e., H E = 0.46; Nybom, ) and for other squash crops, for instance C. pepo ssp. pepo ( H E = 0.500–0.572; Enríquez et al., ; Castellanos‐Morales et al., ), C. maxima ( H E = 0.56; Kaźmińska et al., ), and hybrid Cucurbita rootstocks ( H E = 0.46; Kong et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have assessed the levels of genetic diversity in germplasm collections of domesticated Cucurbita species, mainly in C. pepo (Baranek et al, 2000;Ferriol et al, 2003aFerriol et al, , b, 2004aParis et al, 2003;Cerón et al, 2010;Wu et al, 2011;Formisano et al, 2012;Gong et al, 2012Gong et al, , 2013Inan et al, 2012;Kong et al, 2014;Balvino-Olvera et al, 2017;Kaźmińska et al, 2017;Enríquez et al, 2018;Sánchez-de la Vega et al, 2018;Castellanos-Morales et al, 2019). Few studies have analyzed genetic variation in C. moschata, and these included only a few landraces from Mexico (Montes-Hernández and Eguiarte, 2002;Barboza et al, 2012).…”

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confidence: 99%

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Phylogeographic and population genetic analyses ofCucurbita moschatareveal divergence of two mitochondrial lineages linked to an elevational gradient

Hernández-Rosales

Castellanos‐Morales

Vega

et al. 2020

American J of Botany

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Premise Domestication usually involves local adaptation to environmental conditions. Cucurbita species are a promising model for studying these processes. Cucurbita moschata is the third major crop in the genus because of its economic value and because it displays high landrace diversity, but research about its genetic diversity, population structure, and phylogeography is limited. We aimed at understanding how geography and elevation shape the distribution of genetic diversity in C. moschata landraces in Mexico. Methods We sampled fruits from 24 localities throughout Mexico. We assessed 11 nuclear microsatellite loci, one mtDNA region, and three cpDNA regions but found no variation in cpDNA. We explored genetic structure with cluster analysis, and phylogeographic relationships with haplotype network analysis. Results Mitochondrial genetic diversity was high, and nuclear genetic differentiation among localities was intermediate compared to other domesticated Cucurbita. We found high levels of inbreeding. We recovered two mitochondrial lineages: highland (associated with the Trans‐Mexican Volcanic Belt) and lowland. Nuclear microsatellites show that localities from the Yucatan Peninsula constitute a well‐differentiated group. Conclusions Mexico is an area of high diversity for C. moschata, and these landraces represent important plant genetic resources. In Mexico this species is characterized by divergence processes linked to an elevational gradient, which could be related to adaptation and may be of value for applications in agriculture. The Isthmus of Tehuantepec may be a partial barrier to gene flow. Morphological variation, agricultural management, and cultural differences may be related to this pattern of genetic structure, but further studies are needed.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Phylogeographic and population genetic analyses ofCucurbita moschatareveal divergence of two mitochondrial lineages linked to an elevational gradient

Hernández-Rosales

Castellanos‐Morales

Vega

et al. 2020

American J of Botany

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“…"Candy Roaster" heirloom squash fruits differ in size (10-250+ lbs), shape (round, cylindrical, teardrop, blocky), and color (pink, tan, green, blue, gray, or orange), yet most have fine-textured orange flesh [81], whereas variants of "Cappello da prete," another squash heirloom of the 19th century, still grown in the Po Valley of northern Italy, differ significantly in fruit weight, pulp thickness, rind thickness, peduncle diameter, and seed weight [82]. Using highly polymorphic simple sequence repeats (SSRs) on a collection of 85 winter squash and pumpkin (Cucurbita maxima Duchesne) accessions, Kaźmińka et al [83] noted two distinct clusters, with cluster 1 possessing modern breeding lines and cultivars characterized by small fruits (<5 kg) and bushy or vine growth habit, while cluster 2 contained old cultivars from central and eastern Europe, together with breeding lines and cultivars characterized by large fruits and vine growth habit. The Australian and New Zealand cultivars had longer fruit shelf-life.…”

Section: Assessing Diversity Among Heirloom Germplasmmentioning

confidence: 99%

Pursuing the Potential of Heirloom Cultivars to Improve Adaptation, Nutritional, and Culinary Features of Food Crops

Dwivedi¹,

Goldman

Ortíz

2019

Agronomy

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The burdens of malnutrition, protein and micronutrient deficiency, and obesity cause enormous costs to society. Crop nutritional quality has been compromised by the emphasis on edible yield and through the loss of biodiversity due to the introduction of high-yielding, uniform cultivars. Heirloom crop cultivars are traditional cultivars that have been grown for a long time (>50 years), and that have a heritage that has been preserved by regional, ethnic, or family groups. Heirlooms are recognized for their unique appearance, names, uses, and historical significance. They are gaining in popularity because of their unique flavors and cultural significance to local cuisine, and their role in sustainable food production for small-scale farmers. As a contrast to modern cultivars, heirlooms may offer a welcome alternative in certain markets. Recently, market channels have emerged for heirloom cultivars in the form of farmer-breeder-chef collaborations and seed-saver organizations. There is therefore an urgent need to know more about the traits available in heirloom cultivars, particularly for productivity, stress tolerance, proximate composition, sensory quality, and flavor. This information is scattered, and the intention of this review is to document some of the unique characteristics of heirloom cultivars that may be channeled into breeding programs for developing locally adapted, high-value cultivars.

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“…Among these, SSR markers are preferred because of their abundance in the genome, reproducibility, high level of polymorphism and codominance (Hodel et al, 2016;Powell et al, 1996). Moreover, a high level of synteny among Cucurbita species allows transferability of SSR markers across the genus for various applications (Gong et al, 2008a(Gong et al, , 2013Ka zmi nska et al, 2017;Sim et al, 2015;Stift et al, 2004).…”

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confidence: 99%

Genetic Diversity Among Accessions of Cucurbita pepo Resistant to Phytophthora Crown Rot

Michael¹,

Moon²,

Fu³

et al. 2019

horts

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Phytophthora capsici Leonian, the causal agent of Phytophthora crown rot in squash (Cucurbita pepo L.), is an economically important pathogen worldwide. Currently, no C. pepo cultivars immune to the pathogen are commercially available, but sources of resistance to Phytophthora crown rot have been identified in a set of 16 C. pepo plant introductions (PIs). Knowledge of the genetic relationships among these accessions and their relatedness to economically important morphotypes of C. pepo would inform breeders’ best strategies for introgressing resistance; however, this information is currently lacking. The goal of the current study was to determine genetic diversity among the resistant accessions and their genetic relatedness to susceptible morphotypes of subspecies pepo (Zucchini and Pumpkin) and texana (Acorn, Straightneck, and Crookneck) using 39 SSR markers. The markers revealed 132 alleles averaging 4.40 alleles per locus and had a mean polymorphic information content (PIC) and gene diversity of 0.44 and 0.49, respectively. CMTp235 had the highest PIC and gene diversity of 0.80 and 0.82, respectively. Hierarchical clustering by UPGMA and principal coordinate analysis (PCOA) revealed grouping into two major C. pepo subspecies, texana and pepo, with all the resistant accessions grouping in the latter. In order of increasing genetic distance (GD), the resistant accessions were least distant to Zucchini (GD = 0.34), followed by Pumpkin (GD = 0.40), Crookneck (GD = 0.56), Acorn (GD = 0.60), and Straightneck (GD = 0.61) morphotypes. Mean GD among the resistant accessions was 0.31 and was highest between PIs 615142 and 615132 (0.61). Based on genetic similarity, PIs 174185 and 181761 (disease severity ≤1.4) would be the best sources of resistance for transfer into subspecies texana and pepo, respectively. Overall, the results presented here support a closer genetic relationship between the resistant accessions and morphotypes of subspecies pepo than those of subspecies texana.

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Genetic diversity assessment of a winter squash and pumpkin ( Cucurbita maxima Duchesne) germplasm collection based on genomic Cucurbita -conserved SSR markers

Cited by 33 publications

References 31 publications

Phylogeographic and population genetic analyses ofCucurbita moschatareveal divergence of two mitochondrial lineages linked to an elevational gradient

Phylogeographic and population genetic analyses ofCucurbita moschatareveal divergence of two mitochondrial lineages linked to an elevational gradient

Pursuing the Potential of Heirloom Cultivars to Improve Adaptation, Nutritional, and Culinary Features of Food Crops

Genetic Diversity Among Accessions of Cucurbita pepo Resistant to Phytophthora Crown Rot

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