Genetic diversity assessment of Hemibagrus nemurus from rivers in Java Island, Indonesia using COI gene

Abstract

Abstract. Nuryanto A, Komalawati N, Sugiharto. 2019. Genetic diversity assessment of Hemibagrus nemurus from rivers in Java Island, Indonesia using COI gene. Biodiversitas 20: 2707-2717. Green catfish (Hemibagrus nemurus) is a popular freshwater fish that highly exploited in almost all the rivers in Java Island. The exploited population tends to have low genetic diversity. Meanwhile, separated populations might lead to a genetic difference among the river populations. This study aims to investigate the genetic diversity and population variation of H. nemurus collected at eleven rivers across Java Island. The analysis based on 465 bp fragment of the cytochrome c oxidase 1 gene from 140 individuals. Analysis of overall populations proved that H. nemurus had a high gene diversity (h= 0.935±0.016) and nucleotide diversity (π = 0.073±0.036). Within population analysis also showed that H. nemurus populations showed high levels of gene diversity (h= 0.338±0.128 to 1.000±0.022) and nucleotide diversity (π =0.001±0.001 to 0.071±0.038). Those diversity values indicated that H. nemurus had high level of genetic diversity, except for the Citanduy population. Population comparison showed that significant genetic differences observed among populations (p= 0.000 for both variance component and Fst-value). However, pairwise comparison analysis indicated complex pattern of population differentiation. The high genetic diversity and complex pattern of population differentiation have important implication for H. nemurus conservation in Java Island.

Short Communication: Presence of the vulnerable freshwater goby Sicyopus auxilimentus (Gobiidae, Sicydiinae) on Sangihe Island, Indonesia

“…Partial COI allows the genetic identification of species in numerous taxa. Divergence, which appeared by mutation over time, between different individual's sequences is low for individuals of the same species and high between individuals of a different species (Imtiaz et al 2017;Md-Zain et al 2018a;Md-Zain et al 2018b;Roesma et al 2019;Nuryanto et al 2019). This paper also provides a description based on morphometric, meristic, and molecular data (COI) of S. auxilimentus from Sangihe Island, North Sulawesi, Indonesia.…”

POLIMORFISME GEN MITOKONDRIA 16S IKAN BAUNG (Hemibagrus nemurus Valenciennes, 1840) DARI SUNGAI PROGO, MAGELANG, JAWA-TENGAH

“…Penelitian keragaman genetik ikan baung di Indonesia menggunakan penanda molekuler gen mitokondria masih terbatas. Penggunaan penanda molekuler gen mitokondria 12S dan COIII telah dilakukan oleh Widayanti et al (2019Widayanti et al ( , 2021 dan COI (Nuryanto et al, 2019). Namun demikian belum pernah dilakukan penelitian keragaman genetik ikan baung dari Sungai Progo menggunakan gen mitokondria 16S.…”

Genetic Similarity of Commerson’s Anchovy across Segara Anakan Cilacap Assessed Using Randomly Amplified Polymorphic DNA (RAPD) Markers

“…The value indicates that Commerson's anchovy has high genetic diversity within subpopulations because of the value close to 1. According to Nuryanto et al (2019b), gene diversity value of 1 indicates high gene diversity of a population. High gene diversity of the population based on RAPD markers was also reported in giant gourami (Sari et al, 2014) and Polymesoda erosa from Segara Anakan (Nuryanto & Susanto, 2010).…”

“…The values indicate that all S. commersonnii subpopulations in the Segara Anakan have a high locus diversity of RAPD markers because of the values higher than 0.1. According to Nuryanto et al (2019b), locus diversity of 0.1 indicated high locus diversity. Loci diversity, as obtained in the present study was higher than Indian anchovy (S. indicus), and European anchovy (Viñas et al, 2014;Sukumaran et al, 2019).…”