Genetic Diversity Characterization of Porcine Reproductive and Respiratory Syndrome Virus Isolates in Romania, Based on Phylogenetic Analysis

Zăuleţ, Mihaela; Gurău, Maria Rodica; Petrovan, Vlad; Buburuzan, Laura

doi:10.3390/ijms130912046

Cited by 9 publications

(7 citation statements)

References 25 publications

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“…The authors also suggested multiple introductions of PRRS in their country 26 . A recent study involving relatively low number of sequences revealed that Romanian PRRSV sequences are clustered in three groups within Subtype 1 27 . Another recent paper describing the analysis of PRRSV strains from Slovakia, Czech Republic and a few from Austria revealed that Slovakian strains were clustered into three phylogenetic branches, whereas the Czech and the Austrian ones were mostly forming single nodes on the Subtype 1 part of the tree 28 .…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of PRRSV 1 in Central Eastern Europe in 1994–2014: origin and evolution of the virus in the region

Balka

Podgórska

Brar

et al. 2018

Sci Rep

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More than 20 years after the first outbreaks, the phylogenetic picture of PRRSV is still incomplete and full of gaps, especially in regards of PRRSV 1. Due to the exceptional diversity observed at the eastern borders of Europe and the low number of available sequences from Central Eastern European countries, the authors collected and analyzed both recent as well as already submitted sequences comparing them to a large backbone set of available ORF5 sequences representing the full spectrum of PRRSV 1 Subtype 1 diversity to conduct a systematic phylogenetic analysis and reclassification elucidating the diversity of the virus in these countries. Moreover, further analyses of the EUROSTAT data regarding the live pig movement trends revealed their influence of virus diversity and evolution. The results indicate that besides the effect of local, isolated divergent evolution and the use of modified live vaccines, the most important factor influencing a given country’s virus diversity is the transboundary movement of live, infected animals.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity of PRRSV 1 in Central Eastern Europe in 1994–2014: origin and evolution of the virus in the region

Balka

Podgórska

Brar

et al. 2018

Sci Rep

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“…At present, fluorescent quantitative PCR is primarily used to differentiate various viruses causing reproductive disorders, such as distinguishing PRRSV from African swine fever virus, classical swine fever virus, porcine parvovirus, and pseudorabies virus [ 25 , 26 , 27 ]. Some detection methods can differentiate classical strain from highly pathogenic PRRSV strains [ 28 ], while other detection methods can distinguish between the two genotypes PRRSV1 and PRRSV2 [ 29 , 30 ]. In this study, we developed a multiple real-time PCR method for the detection and identification of the four subtypes of PRRSV strains that exist in China.…”

Section: Discussionmentioning

confidence: 99%

Development and Implementation of a Quadruple RT-qPCR Method for the Identification of Porcine Reproductive and Respiratory Syndrome Virus Strains

Ruan,

Ren,

et al. 2023

Viruses

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Background: Porcine reproductive and respiratory syndrome virus (PRRSV) causes porcine reproductive and respiratory syndrome (PRRS), leading to abortion in sows and respiratory distress in breeding pigs. In China, PRRSV1 and PRRSV2 are the two circulating genotypes in swine herds, with distinct virulence. PRRSV2 further consists of classical (C-PRRSV2), highly pathogenic (HP-PRRSV2), and NADC30-Like (N-PRRSV2) subtypes. The diversity of PRRSV poses challenges for control and eradication, necessitating reliable detection assays for differentiating PRRSV genotypes. Methods: A new TaqMan-based RT-qPCR assay with four sets of primers and probes targeting conserved regions of the ORF7 and NSP2 genes of PRRSV was developed, optimized, and evaluated by us. Reaction conditions such as annealing temperature, primer concentration, and probe concentration were optimized for the assay. Specificity, sensitivity, repeatability, stability, limit of detection (LOD), concordance with the reference method were evaluated for the assay. Results: The assay could detect and type PRRSV1, C-PRRSV2, HP-PRRSV2, and N-PRRSV2 simultaneously with 97.33% specificity, 96.00% sensitivity, 12 copies/μL LOD, 97.00% concordance with reference assays. We applied the assay to 321 clinical samples from swine farms in China. The assay successfully detected and typed 230 PRRSV-positive samples, with 24.78% (57/230) of them further confirmed by ORF5 gene sequencing. The prevalence of PRRSV subtypes among the positive samples was as follows: C-PRRSV2 (15.22%), HP-PRRSV2 (23.48%), and N-PRRSV2 (61.30%). Two samples showed coinfection with different PRRSV subtypes. Conclusion: The quadruple RT-qPCR assay is a powerful tool for detecting and typing the currently circulating PRRSV strains in Chinese swine populations. It can assist in the surveillance of PRRSV prevalence and the implementation of prevention and control strategies.

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“…Next, we performed PCR analysis using specific primers used for genotyping (Gallardo, 2009). The DNA sequencing reactions were performed as described previously (Zaulet, 2012). Briefly, the reaction products were purified using Wizard® PCR Preps DNA Purification System (Promega, Madison, WI, USA), and the concentration and purity of the products were evaluated by spectrophotometry (Eppendorf BioPhotometer, Hamburg, Germany).…”

Section: Methodsmentioning

confidence: 99%

Genetic characterization of African swine fever virus in Romania during 2018-2019 outbreak

Petrovan

Turcitu

Matei³

et al. 2019

Preprint

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49African swine fever (ASF) is a highly contagious and lethal viral disease of swine with 50 significant socio-economic impact in the developed and developing world. Since its 51 reintroduction in 2007 in the Republic of Georgia, the disease has spread dramatically thorough 52 Europe and Asia. Among the most affected countries in Europe is Romania, which initially 53 reported the disease in 2017 and in 2018-2019 lost about 1 million pigs. There is no molecular 54 characterization of the virus circulating in Romania during that reported period; therefore, the 55 purpose of this study was to provide an initial molecular characterization using samples collected 56 from two farms affected by ASFV. The causative strain belongs to genotype II, and its closest 57 relatives are the strains circulating in Belgium, Russia, and China. 58 59 type of article: short communication 60 61 62 63 64 65 66 67

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Genetic Diversity Characterization of Porcine Reproductive and Respiratory Syndrome Virus Isolates in Romania, Based on Phylogenetic Analysis

Cited by 9 publications

References 25 publications

Genetic diversity of PRRSV 1 in Central Eastern Europe in 1994–2014: origin and evolution of the virus in the region

Genetic diversity of PRRSV 1 in Central Eastern Europe in 1994–2014: origin and evolution of the virus in the region

Development and Implementation of a Quadruple RT-qPCR Method for the Identification of Porcine Reproductive and Respiratory Syndrome Virus Strains

Genetic characterization of African swine fever virus in Romania during 2018-2019 outbreak

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