Genetic Diversity Evaluation and Population Structure Analysis of Red Swamp Crayfish (Procambarus clarkii) from Lakes and Rice Fields by SSR Markers

Abstract

The red swamp crayfish (Procambarus clarkii) is an important aquatic animal and has developed as a popular aquaculture species in China. In this study, a total of 72,839 SSR motifs were identified from transcriptional data, and 20 microsatellite markers of them were finally developed to assess the genetic diversities of seven wild populations from natural lakes and nine cultured populations from rice fields. Genetic diversity was slightly higher in the cultured populations than in the wild populations. The degree of genetic differentiation between cultured populations is slight, while a moderate to a large degree of genetic differentiation between wild populations and most of the variations occurred within individuals (79%). The analysis of cluster, principal coordinate analysis and STRUCTURE were similar, and they showed that isolation-by-distance pattern was not significant. The microsatellite markers developed in this study can not only be used for genetic monitoring of population but also provide important information for the management of breeding and cultured population in red swamp crayfish.

Cardiac Transcriptome and Histology of the Heart of the Male Chinese Mitten Crab (Eriocheir sinensis) under High-Temperature Stress

“…SSR markers are codominant, multi-allelic, and useful tools for genetic, evolutionary, and polymorphism studies [31]. In this study, 28,916 SSRs were detected within E. sinensis unigenes.…”

Genetic Diversity and Population Structure of Chinese Longsnout Catfish (Leiocassis longirostris) Using Microsatellite DNA Markers

“…Microsatellites, or simple sequence repeats (SSRs), are short regions of 2-6 base tandem repeats observed in all prokaryotic and eukaryotic genomes [8,9]. Due to the characteristics of microsatellites and the convenience of analysis using PCR, they have been applied to evaluate the genetic diversity and population structure of numerous aquatic animals [10,11], such as walleye pollock (Theragra Chalcogramma) [12], large yellow croaker (Larimichthys crocea) [13], gibel carp (Carassius auratus gibelio Bloch) [14], hard clam (Meretrix meretrix) [15], and red swamp crayfish (Procambarus clarkii) [16,17].…”