Genetic diversity in Egyptian and Italian goat breeds measured with microsatellite polymorphism

Agha, Saif; Pilla, Fabio; Galal, Salah; Shaat, I.; D’Andrea, Mariasilvia; Reale, Stefano; Abdelsalam, Ahmed M.; Li, Menghua

doi:10.1111/j.1439-0388.2008.00730.x

Cited by 69 publications

(49 citation statements)

References 26 publications

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“…Therefore the loci examined in this study were highly informative. The mean number of alleles per loci (13.33) were higher than those reported by Saitbekova et al 38 , Agha et al 39 Genetic Diversity of Three ... et al 40 , Fatima et al 41 , Oliveira et al 42 , Qi et al 43 , lower than those reported by Serrano et al 33 , Caňón et al 44 and similar to those reported by Missohou et al 37 , Barker at al. 45 , Chenyambuga et al…”

Section: Discussioncontrasting

confidence: 53%

“…Observed and expected heterozygosities estimated in this study were higher than those found in most studies 12,38,39,52,53,[57][58][59] . The values were also higher than those reported for Abaza (0.772), Angora (0.750), Gurcu (0.751) and Hair goat breeds (0.722) raised in Turkey 43 .…”

contrasting

confidence: 51%

“…The mean number of alleles/locus/breed found in this study (between 10.33 and 11.33) were similar to those found by Bozkaya et al 49 , Korkmaz-Ağaoğlu and Ertuğrul 50 and higher than those reported by Behl et al 35 , Saitbekova et al 38 , Agha et al 39 , Barker et al 45 , Li et al 48 , Gour et al 51 , Jandurova et al…”

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confidence: 33%

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Genetic Diversity of Three Native Goat Populations Raised in the South-Eastern Region of Turkey

Gürler¹,

Bozkaya²

2013

Kafkas Univ Vet Fak Derg

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The aim of this study was to investigate the genetic variability of three goat populations raised in the Southeastern region of Turkey by using microsatellite loci defined in gazelle (Gazella granti). DNA was isolated from 120 blood samples collected from goats raised in Sanliurfa (n = 40), Kilis (n = 40) and Siirt (n = 40) provinces. Eight microsatellite loci (33HDZ8, 33HDZ290, 33HDZ433, 33HDZ496, 33HDZ593, 33HDZ692, 33HDZ749, 33HDZ974) were used for amplification with polymerase chain reaction (PCR). Fragment lengths of the amplifed loci were analysed using a capillary electrophoresis system. Six of the eight loci (75%) were successfully amplified and four (66.7%) of them were polymorphic. Three of the polymorphic loci (33HDZ496, 33HDZ749 ve 33HDZ974) were used for estimating genetic parameters. A total of 40 alleles were detected. Number of alleles and polymorphism information contents (PIC) for the loci 33HDZ496, 33HDZ749 and 33HDZ974 Keywords: Goat, Microsatellite, Genetic diversity Güney Doğu Anadolu Bölgesi'nde Yetiştirilen Üç Keçi Populasyonunda Genetik Çeşitlilik ÖzetBu çalışma bir ceylan türünde (Gazella granti) tanımlanmış sekiz mikrosatelit lokusunu kullanarak Güneydoğu Anadolu Bölgesinde yetiştirilen üç keçi populasyonunu genetik çeşitlilik yönünden değerlendirmek amacıyla yapılmıştır. Bu amaçla, Şanlıurfa (n = 40), Kilis (n = 40) ve Siirt (n = 40) illerinde yetiştirilen üç keçi populasyonundan toplam 120 adet kan örneği alınmış ve bu örneklerden DNA izole edilmiştir. İzole edilen DNA'lar sekiz mikrosatelit lokusuna (33HDZ8, 33HDZ290, 33HDZ433, 33HDZ496, 33HDZ593, 33HDZ692, 33HDZ749, 33HDZ974) ait primerler kullanılarak PCR ile çoğaltılmış ve PCR ürünleri kapillar elektroforeze tabi tutularak fragman uzunluğu analizi gerçekleştirilmiştir. Sekiz lokustan altısı (%75) PCR ile amplifiye olmuş ve bunlardan dördü (%66.7) polimorfik bulunmuştur. Polimorfik lokusların üçü (33HDZ496, 33HDZ749 ve 33HDZ974) genetik parametrelerin hesaplanmasında kullanılmıştır. Çalışmada toplam 40 allel tespit edilmiştir. 33HDZ496, 33HDZ749 ve 33HDZ974 lokusları için sırasıyla 13, 15 ve 12 allel gözlenmiş olup, herbir lokus için hesaplanan polimorfizm bilgi içeriği (PIC) aynı sırayla 0.836, 0.821 ve 0.817 olarak belirlenmiştir. Şanlıurfa, Kilis ve Siirt populasyonları için ortalama allel sayıları sırasıyla 10.33±1.15, 10.67±2.08 ve 11.33±0.58, etkili allel sayıları ise yine aynı sıra ile 6.131±1.42, 5.823±0.23 ve 5.870±0.75 olarak bulunmuştur. Şanlıurfa, Kilis ve Siirt populasyonları için ortalama gözlenen heterozigotluk oranları sırasıyla 0.827±0.083, 0.808±0.044 ve 0.895±0.069, beklenen heterozigotluk oranları ise yine aynı sıra ile 0.843±0.043, 0.841±0.007 ve 0.838±0.023 olarak belirlenmiştir. 33HDZ496, 33HDZ749 ve 33HDZ974 lokusları için hesaplanan FIS değerleri sırasıyla 0.0164, 0.0245 ve 0.0240 olarak bulunmuştur. Tüm populasyonlar için hesaplanan FST değeri (0.009) incelenen populasyonda gözlenen genetik çeşitliliğin %99'unun bireyler arasındaki farklılıktan kaynaklandığını göstermiştir. Çalışma sonuçları incelenen ke...

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Section: Discussioncontrasting

confidence: 53%

contrasting

confidence: 51%

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Genetic Diversity of Three Native Goat Populations Raised in the South-Eastern Region of Turkey

Gürler¹,

Bozkaya²

2013

Kafkas Univ Vet Fak Derg

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“…On the other hand, the most known goat breed in Syria is the Damascus goat, known sometimes as Shami. In other nearby countries, the common native goat breed is the Black Bedouin in Palestine (Silanikove, 1986) and Egyptian Baladi or Bedouin goat in Egypt (Agha et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Genetic relatedness between Ardi, Black Bedouin and Damascus goat breeds

Al-Atiyat¹,

Aljumaah²

2014

Genet. Mol. Res.

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ABSTRACT.The present study aimed to analyze genetic relatedness and differentiation of common native goat populations in some countries of the Middle East. The populations were Ardi, Black Bedouin, and Damascus goats in the Kingdom of Saudi Arabia, Jordan, and Syria, respectively. Domesticated goats of the Middle East are mostly related to common ancestors, but there is limited molecular genetic evidence. Four microsatellite DNA markers were genotyped in 89 individuals of the three populations using an automated genetic analyzer. Ardi, Black Bedouin, and Damascus goats exhibited high average allele number and expected heterozygosity of 8.25, 9, and 7.25, and 0.750, 0.804, and 0.779, respectively. F-statistics for population differentiation showed 6.0% of total genetic variation, whereas 94.0% as differentiation between individuals within all populations. The least varied within populations was Ardi goats, then Damascus goats and finally Black Bedouin goats. Furthermore, the Damascus goat population was more differentiated from Black Bedouin goats than from Ardi goats. On the other hand, there was strong evidence of admixture between the majority of Ardi and Black Bedouin goat individuals but little with those of Damascus Goat genetic relatedness and phylogeny goats. Genetic distance between Ardi and Black Bedouin goats was the shortest, whereas it was the longest between Ardi and Damascus goats. The phylogenetic tree clearly revealed the expected degree of differentiation in the three populations. From a genetic conservation point of view, it is recommended to maintain the biodiversity of these distinct populations in case genetic migration of genetic resources and genetic conservation are absent.

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“…Microsatellites have been commonly utilized for the assessment of genetic diversity, construction of genetic maps, quantitative trait loci mapping and parentage testing etc. (Batendse et al 1997, Buchanan et al 1994, Jin et al 2005, Agha et al 2008, Manatrinon et al 2008, Ślaska et al 2008, Kusza et al 2010. Li et al (2002) analyzed genetic relationships among twelve Chinese indigenous goat populations based on 26 microsatellite markers.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Tibetan goats of Plateau type using microsatellite markers

Wang

et al. 2011

Arch. Anim. Breed.

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The 10 microsatellite markers (XBM7, XBM11, XBM16, XBM19, XBM24, XBM31, XBM84, TGLA53, SRCRSP-10 and ILS005) were selected to investigate the genetic diversity of Tibetan goat of Plateau type (NM, n=108), and the other 5 goat populations, i.e., Tibetan goat of Valley type (TG, n=36), Baiyu black goat (BY, n=36), Jianchang black goat (JC, n=36), Meigu goat (MG, n=36) and Xinjiang goat (XJ, n=32) were served as control. The mean polymorphism information content, heterozygosity and effective allele number of these 6 populations were 0.660/0.777/4.476, 0.716/0.797/4.9416, 0.631/0.673/3.061, 0.649/0.680/3.125, 0.629/0.680/3.125 and 0.561/0.793/4.840 respectively. The allele frequencies of Tibetan goat of plateau type in 10 microsatellite loci were greatly different with other 5 goat populations. The 6 goat populations were grouped into three distinct clusters: the Tibetan goat cluster (NM goat and TG goat), Sichuan goat cluster (JC goat, MG goat and BY goat), and Xinjiang goat cluster (XJ goat). These 3 distinct clusters were finally clustered together. The genetic differences among populations were in accordance with their geographical and historical origins.Keywords: Microsatellite DNA, genetic diversity, Tibetan goat Zusammenfassung Genetische Diversität von Tibetischen Hochplateau-Ziegen unter Verwendung von MikrosatellitenmarkernDie 10 Mikrosatellitenmarker (XBM7, XBM11, XBM16, XBM19, XBM24, XBM31, XBM84, TGLA53, SRCRSP-10 und ILS005) wurden ausgewählt, um die genetische Diversität von Tibetischen Hochplateau-Ziegen (NM, n=108) zu untersuchen, und die anderen 5 Ziegenpopulationen, d. h. Tibetische Tal-Ziege (TG, n=36) schwarze Baiyu-Ziege (BY, n=36), schwarze JianchangZiege (JC, n=36), Meigu-Ziege (MG, n=36) und Xinjiang-Ziege (XJ, n=32) und Xinjian-Ziege (XJ, n=32) dienten als Kontrolle. Der durchschnittliche Polymorphismus-Informationsgehalt, die Heterozygosität und die effektive Allee-Anzahl dieser 6 Populationen betrugen 0,660/0,777/4,476, 0,716/0,797/4,9416, 0,631/0,673/3,061, 0,649/0,680/3,125, 0,629/0,680/3,125 bzw. 0,561/0,793/4,840. Die Allelhäufigkeiten von Tibischen Hochplateau-Ziegen an 10 Mikrosatelliten-Loci unterscheiden sich sehr stark von den anderen 5 Populationen. Die 6 Ziegenpopulationen wurden in 3 distinkte Cluster gruppiert: das Cluster Tibetische Ziege (NM-und TG-Ziege), das Cluster Sichuan-Ziege (JC-, MG-und BY-Ziege) und das Cluster Archiv Tierzucht 54 (2011) 2, 188-197, ISSN 0003-9438

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Genetic diversity in Egyptian and Italian goat breeds measured with microsatellite polymorphism

Cited by 69 publications

References 26 publications

Genetic Diversity of Three Native Goat Populations Raised in the South-Eastern Region of Turkey

Genetic Diversity of Three Native Goat Populations Raised in the South-Eastern Region of Turkey

Genetic relatedness between Ardi, Black Bedouin and Damascus goat breeds

Genetic diversity of Tibetan goats of Plateau type using microsatellite markers

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