Genetic diversity in local and commercial dry bean (Phaseolus vulgaris) accessions based on microsatellite markers

Abstract

ABSTRACT. Dry beans are considered to be a crop of great socioeconomic importance, because they are an inexpensive source of nutrients and because their cultivation requires considerable manual labor. Studies of genetic diversity have been very important for genetic improvement programs, because they give parameters for the identification of genitors that can provide large heterosis effects and improved segregation in recombinants, increasing the probability of obtaining superior genotypes in the progeny. We evaluated the genetic diversity of 57 dry bean accessions, including 31 local accessions, propagated by small-scale farmers, 20 accessions supplied by the Brazilian Agricultural Research Agency, and six commercial accessions, using 16 microsatellite primers. Among these primers, 13 were found to be polymorphic, giving 29 polymorphic alleles. The largest number of alleles per locus was observed for primer BM141, which had four alleles. The polymorphic information content varied from 0.11 to 0.51, observed for loci BM212 and BM141, respectively. The lowest degree Genetic diversity in common bean of dissimilarity (0.0) was found between the accession Iapar 81 and the accessions E03, E04, E09, and E13 and between the accession pairs E08 with E16 and Iapar 31 with E06. The highest degree of dissimilarity was found between the accessions Carioca and E22 (1.0). Grouping analysis revealed four groups, according to the place of origin. This tendency was also found in the principal coordinate analysis. The local genotypes were found to have relatively high genetic diversity, while the EMBRAPA and commercial cultivars had a relatively narrow genetic basis.

Microsatellite markers for assessing genetic diversity of the medicinal plant Paris polyphylla var. chinensis (Trilliaceae)

“…Ever since their development, they have constantly been modified to enhance their utilizations and to be more and more important for the analyses of the genome and genetic diversity (Vieira et al, 2007;Gonçalves et al, 2009;Oliveira et al, 2010). Molecular markers permit us to make estimates of genetic diversity directly at the DNA level, reduc ing the interference of environmental factors (Cabral et al, 2011). Also, many markers are available for each genome, there are high levels of polymorphism, and they are free of pleiotropic effects (Leal et al, 2010).…”

“…Also, many markers are available for each genome, there are high levels of polymorphism, and they are free of pleiotropic effects (Leal et al, 2010). During recent decades, SSRs (simple sequence repeats), also known as microsatellites, have become the most popular source of genetic markers owing to their high reproducibility, multi-allelic nature, codominant inheritance, high abundance, and wide genome coverage (Sharma et al, 2007;Demir et al, 2010;Cabral et al, 2011). The high infor mation content in SSR loci, together with their codominant expression, make SSRs ideal for genomic mapping, population genetics, conservation biology, marker-assisted selection, and other studies (Roa et al, 2000;Ellegren, 2004;Schlötterer, 2004;Leal et al, 2010).…”

Diversity and genetic analysis through DArTseq in common bean(Phaseolus vulgaris L.) germplasm from Turkey

“…Similarly, 112 polymorphic amplified fragment length polymorphism (AFLP) bands (Maras et al, 2008) were produced to detect genetic diversity within a set of 29 common bean accessions. Cabral et al (2011) used simple sequence repeat (SSR) markers to identify genetic diversity in dry bean accessions to detect 29 polymorphic alleles. However, the use of these markers has generated a limited number of polymorphic bands from which to determine the genetic diversity (Gaitán-Solís et al, 2002;Svetleva et al, 2006;Gill-Langarica et al, 2011;De La Fuente et al, 2013) and, clearly, a larger number of markers are needed to cover the genome and assess genetic diversity for common bean.…”

Molecular characterization of Anthurium genotypes by using DNA fingerprinting and SPAR markers

“…Molecular markers are valuable tools for estimating genetic diversity (Vieira et al, 2007;Gonçalves et al, 2009;Oliveira et al, 2010;Cabral et al, 2011). These markers can be used to directly measure genetic diversity at the DNA level, eliminating the influence of environmental variations.…”