Genetic diversity in natural populations of Theobroma subincanum Mart. in the Brazilian Amazon

Rivas, Luiz Henrique; Giustina, L.D.; Luz, Lucas Nunes da; Karsburg, Isane Vera; Pereira, Telma Nair Santana; Rossi, Ana Aparecida Bandini

doi:10.4238/2013.october.24.12

Cited by 15 publications

(18 citation statements)

References 16 publications

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“…In the level of species, the genetic diversity was H=0,27 and I=0.41 (Table 2). Similar results were found by many works that studied native species from Amazon, such as Mauritia flexuosa (ROSSI et al, 2014), Theobroma subincanum (RIVAS et al, 2013) and Theobroma speciosum Willd. Ex Spreng (GIUSTINA et al, 2014).…”

Section: Resultssupporting

confidence: 78%

“…Santana et al (2011), observed an average of 10 bands per primer when studied 17 accessions of umbu-cajazeiras belonging to BAG tropical fruit of Embrapa Mandioca e Fruticultura, based on ISSR markers. However, Rivas et al (2013), studied native populations of Theobroma subincanum Mart. and obtained results lower than those found in the present study, with the average number of bands per primer of 6,69.…”

Section: Resultsmentioning

confidence: 99%

“…Among the methods that use molecular markers, the ISSR (Inter Simple Sequence Repeat) has been widely used because it demonstrates a fast and efficient technique with high reproducibility and high indices of polymorphism, providing a large number of low cost data for the researcher and the ISSR markers may be transferred to any kind of plant (RIVAS et al, 2013;GIUSTINA et al, 2014;ROSSI et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Diversidade Genética E Estrutura Populacional De Jatobá: Uma Espécie Com Potencial Econômico Para a Amazônia

Tiago¹,

Rossi²,

Carpejani³

et al. 2018

Ciênc. Florest.

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Jatobá (Hymenaea courbaril L.) is a species that shows ecological importance and occurs in different geographic regions in Brazil. Knowing and evaluating the existence of places with higher genetic variability can help in conservation programs and actions. In this context, the present study aimed to evaluate the genetic diversity and structure in natural populations of Jatobá with occurrence in Mato Grosso Amazon through ISSR markers. Fifty-four individuals were sampled in three populations, being one in the municipality of Marcelândia (MA=24) and two in the municipality of Alta Floresta (Comunidade Central AF=17 and Pista do Cabeça PC=13 individuals). Total genomic DNA was extracted from the leaf tissue by the CTAB method. The 54 individuals were genotyped with 10 ISSR primers. A total of 110 fragments were amplified, being 78.2% polymorphic. The greatest diversity indices were found in the population from MA (H=0.25; I=0.37 and %P=65.45). The genetic distance was higher among the populations of the two municipalities (0.17 between MA and AF; 0.16 between MA and PC). The analysis of molecular variance (AMOVA) indicated that 31.79 % of the total variance is among populations and 68.21 % within populations. There is genetic diversity in native populations of Jatobá in Mato Grosso Amazon. We recommend that individuals from both populations be preserved, in order to ensure the maintenance of genetic variability and the effective conservation of species in Mato Grosso Amazon.

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Section: Resultssupporting

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Diversidade Genética E Estrutura Populacional De Jatobá: Uma Espécie Com Potencial Econômico Para a Amazônia

Tiago¹,

Rossi²,

Carpejani³

et al. 2018

Ciênc. Florest.

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“…However, sapucaia is known to be a long-life tree species, and is preferably allogamous, its pollen is spread by bees and its seeds are spread by animals and humans (Mori and Prance, 1990). The results of some studies have shown that genetic distance does not always correlate with geographic distance (Rivas et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Investigating genetic diversity in sapucaia using inter simple sequence repeat markers

Borges¹,

Santos²,

Maia³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Sapucaia is a tree species originating from the Brazilian Amazon and is widely distributed in Brazil, especially in the mid-north region (Piauí and Maranhão states). Its seeds are rich in calories and proteins, and possess great potential for commercialization. Little is known about the genetic variability in the germplasm of most Lecythis species. Here, 11 inter-simple sequence repeat primers were used to estimate the genetic variability among 17 accessions, and to determine the levels of genetic variation and the standards of population structure in sapucaia. The accessions were obtained from the active germplasm bank (AGB) of Embrapa Meio-Norte, Teresina, PI, Brazil, and corresponded to four occurrence areas. Ninety-six loci were analyzed 2 R.C. Borges et al. Genetics and Molecular Research 15 (3): gmr.15038565 among the studied individuals. High variation was found at the species level, where the percentage of polymorphic bands was 94.79%, Nei's genetic diversity (h) was 0.3110, and Shannon's index (I) was 0.4732. In the analyzed populations, the percentage polymorphism ranged from 20.83 to 94.79%, Nei's genetic diversity ranged from 0.0863 to 0.2969, and Shannon's index ranged from 0.1260 to 0.4457. Significant genetic differentiation was detected among the populations (Φ ST = 10.66%); however, the greatest genetic differentiation was found within the populations (89.34%), between which there was an intermediate level of gene flow (N m = 1.10). Accessions BGS 2 and BGS 4 were the most divergent, whereas accessions BGS 14 and BGS 15 were the most similar. Therefore, sapucaia analyzed from the AGB present an elevated level of genetic diversity and may have potential use in genetic breeding programs.

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“…Inter-simple sequence repeats (ISSRs) are dominant and represent genetic variation within microsatellite regions in the genome. They have been used to estimate genetic diversity and genetic parameters of populations of multiple plant species (Rivas et al, 2013;Lorenzoni et al, 2014;Rossi et al, 2014;Silva et al, 2014;Ghariani et al, 2015;ArrigoniBlank et al, 2016;Shen et al, 2016). The advantage of ISSRs is the possibility to obtain a high number of polymorphic loci without the need of genome sequence knowledge (Faleiro, 2007).…”

Section: Introductionmentioning

confidence: 99%

Genetic variability analysis of Byrsonima crassifolia germplasm collected in Pará State using ISSR markers

Rodrigues¹,

Moura²,

Ramos³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Native of the Amazon, the nanche (Byrsonima crassifolia) is a fruit cultivated by family farmers and used in cooking; as such, it represents an opportunity for regional agribusiness. The Embrapa Eastern Amazon set up an active germplasm bank (BAG) consisting of 22 accessions sampled in 11 municipalities of Pará State. Due to its economic potential, there is an interest to advance the genetic breeding program of this species. The aim of this study was to characterize the BAG nanche collection using inter-simple sequence repeat (ISSR) markers. Accessions were genotyped using 23 preselected ISSR primers resulting in 109 amplified polymorphic and 51 monomorphic bands. With eight polymorphic bands each, the most polymorphic primers were UBC 809 and UBC 848. An unweighted pair-group method with arithmetic average cluster analysis based on Jaccard's coefficient indicated that the individuals clustered into two distinct groups. Accessions Igarapé Açu-2 and Augusto Corrêa-Pl 1 were most similar. The genetic dissimilarity values ranged from 0.10 to 0.59. We conclude that the ISSR markers were efficient in detecting polymorphisms in the nanche accessions, and that it is possible to infer the genetic variability among accessions of the collection. This demonstrate the importance of using molecular markers in poorly studied species and the advantages that this information can bring to the genetic improvement of such species.

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Genetic diversity in natural populations of Theobroma subincanum Mart. in the Brazilian Amazon

Cited by 15 publications

References 16 publications

Diversidade Genética E Estrutura Populacional De Jatobá: Uma Espécie Com Potencial Econômico Para a Amazônia

Diversidade Genética E Estrutura Populacional De Jatobá: Uma Espécie Com Potencial Econômico Para a Amazônia

Investigating genetic diversity in sapucaia using inter simple sequence repeat markers

Genetic variability analysis of Byrsonima crassifolia germplasm collected in Pará State using ISSR markers

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