Genetic diversity in Passiflora species determined by morphological and molecular characteristics

Abstract

Morphological and molecular characteristics were studied in six wild species of Passiflora. There were statistically significant differences among these six species for all characteristics studied. Intra-specific variability was observed for number of flowers, number of fruits, number of seeds, fruit length, fruit width and leaf area. Cluster analysis using morphological data showed three groups: 1) P. palmeri var. sublanceolata, P. morifolia and P. foetida var. foetida, 2) P. coriacea and P. micropetala, and 3) P. suberosa. The dendrogram constructed using randomly amplified polymorphic DNA (RAPD) data showed six different groups for each species. The genetic distances among the 24 accessions ranged from 0.05 (between P. morifolia accessions P1 and P3) to 0.95 (P. coriacea accession 31 and P. palmeri var. sublanceolata accession 49). The species showed high morphological and molecular inter-and intraspecific variability.

Morphoagronomic and molecular characterization of ornamental passion fruit cultivars

“…From the total number of markers, 82% were polymorphic in RAPD, and 91.2% were polymorphic in ISSR, showing the highgenetic variability among the six cultivars. A high polymorphism indicating the high-genetic variability of the Passiflora genus by RAPD markers has already been reported by Bellon et al (2009Bellon et al ( , 2014, Viana et al (2010), Castro et al (2011), andCerqueira-Silva et al (2012). This fact have been verified also by Santos et al (2011b), Costa et al (2012), and Sousa et al (2015) by using ISSR markers.…”

“…2017 DOI: 10.1590/S0100-204X2017001000004 descriptors ( Figures 1 A and 2 A), and also proved the importance of using morphoagronomic descriptors to differentiate cultivars through genetic variability. The worth of using descriptors for the analysis of differentiation was also observed by Viana et al (2010), Santos et al (2011aSantos et al ( , 2014 and Paiva et al (2014) when studying the Passiflora genus. The validation rate, that is, the percentage of coincidence between obtained descriptors and the cultivar descriptors, used in the protection requests, was high in different ornamental passion fruit cultivars (100% for 'BRS Rosea Púrpura', 97% for 'BRS Pérola do Cerrado', 96% for 'BRS Céu do Cerrado', 90% for 'BRS Estrela do Cerrado', 81% for 'BRS Rubiflora', and 71% for 'BRS Roseflora').…”

“…Studies on the characterization of genetic diversity using the morphological and agronomic characteristics of Passiflora have been reported in the literature (Araújo et al, 2008;Santos et al, 2011a;Machado et al, 2015). Other studies have also been carried out by associating molecular markers (Viana et al, 2010).…”

Genetic variability assessment in the genus Passiflora by SSR marker

“…Crochemore et al (2003) used RAPD markers and found a clear separation and a significant diversity among the 11 species. Viana et al (2010) used both RAPD and morphological markers to quantify the variability among six species of this genus. However, no relationship was observed among the species.…”

“…Thus, highly informative content can be generated (Schlötterer, 2004). Comparative studies on Passiflora have been very successful when using Randomly Amplified Polymorphic (RAPD) molecular markers (Viana et al, 2003;Pérez et al, 2007;Viana et al, 2010), inter-simple sequence repeat (ISSR) (Santos et al, 2011), and chloroplast genes (Yockteng and Nadot, 2004). However, there are only a few studies on the use of microsatellite markers for the characterization of Passiflora involving several species.…”