Genetic diversity in pearl millet restorers based on seed vigour traits and SSR markers

Abstract

Thirty four pearl millet restorer lines were evaluated on the basis of nine seed vigour traits and molecular analysis was done with 55 SSRs to study the genetic divergence among them. Cluster analysis based on seed vigour parameters revealed the considerable amount of variability and all genotypes were divided into six clusters. Furthermore, a set of 39 SSRs, selected after initial screening of 55, amplified 226 alleles with a mean of 5.84 alleles per locus. The highest polymorphic information content (PIC) value obtained was for PSMP 2084 (0.88) with a range of 0 to 0.88 and average PIC of 0.558. Neighbor joining tree based on molecular data differentiated all the genotypes into two major groups. The grouping based on both approaches can help breeders to use the studied material in further breeding for improvement of germination potential and early seedling vigour of pearl millet.

Molecular Analysis of Genetic Variability and Relationship among Pearl Millet (Pennisetum glaucum) Genotypes by Using SSR Markers

“…of amplicons varied from 1 to 6 with an average of 3.5 per primer. The number of polymorphic amplicons ranged from 7 (PSMP 2018) to 19 (PSMP 2076) with an average of 13 bands per primerA similar study was reported by [3]. PSMP 2076 showed the highest PIC (0.69), while it was lowest for PSMP 2018 (0.37) with an average of 0.44 as mentioned in Table 2.…”

“…C2-HHB 197 and C4-Sampada genotypes have the highest similarity coefficient 0.76. Among all the genotypes, C1-HHB 67 and V5-PC 612 was found most diverse as it separated from all other genotypes at a very low similarity coefficient of 0.6 as mentioned in Table 3.A similar study was reported by [3]. A similar type of report of his DNA fingerprinting of pearl millet using molecular markers has also been reported [18] Simple sequence repeats are tandem repeats of approximately 6 nucleotides in both coding and noncoding regions.…”

“…(L.) Gaertn), Foxtail millet (Setariaitalica (L.) Beauv) and Proso millet (Panicum miliaceum L.). Pearl millet assumes a basic part in food security" [3]. It has amazing properties like high photosynthetic proficiency, high dry matter creation limit, early development, dry season resilience, the necessity of insignificant buy inputs, and general liberation from biotic and abiotic stresses [2,4].…”