Genetic Diversity in the Camel Tick &amp;lt;i&amp;gt;Hyalomma dromedarii&amp;lt;/i&amp;gt; (Acari: Ixodidae) Based on Mitochondrial Cytochrome c Oxidase Subunit I (COI) and Randomly Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR)

Al-Deeb, Mohammad Ali; Enan, Mohamed R.

doi:10.4236/ae.2018.64021

Cited by 4 publications

(4 citation statements)

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“…The 16S rRNA and cox1 are useful markers for tick taxonomy at the species level [22,24]. So far, very few studies in the UAE have provided molecular records of ticks [25,26]. For example, H. dromedarii was identified based on morphology, and its identification was confirmed based on the use of the cox1 gene [25].…”

Section: Introductionmentioning

confidence: 99%

“…For example, H. dromedarii was identified based on morphology, and its identification was confirmed based on the use of the cox1 gene [25]. Furthermore, the genetic diversity of several populations of H. dromedarii in the UAE was determined using the cox1 gene and Randomly Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR) [26]. Thus, molecular tools provide an opportunity to correctly identify tick fauna in the UAE.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence, Distribution, and Molecular Record of Four Hard Ticks from Livestock in the United Arab Emirates

Perveen

Muzaffar

Al-Deeb

2021

Insects

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Ticks are important arthropod vectors that serve as reservoirs of pathogens. Rapid urbanization and changes in animal breeding practices could be causing a rise in tick burden on animals. Studies on tick distribution on livestock and tick molecular diversity from the United Arab Emirates (UAE) are limited. The aim of this study was to (i) provide molecular and morphological identification of tick species, (ii) compare tick infestation between different hosts, (iii) compare tick infestation in relation to the sex of the host, and (iv) assess the prevalence of tick species on hosts. A total of 5950 ticks were collected from camels (4803 ticks), cows (651 ticks), goats (219 ticks), and sheep (277 ticks). Ticks were identified based on morphological characters at the species level using taxonomic keys. In addition, Polymerase Chain Reaction (PCR) amplification of the cytochrome oxidase subunit 1 (cox1) and 16S rRNA mitochondrial genes was used to identify ticks. Four species were confirmed based on molecular and morphological characterization, namely, Hyalomma dromedarii, Hyalomma anatolicum, Rhipicephalus sanguineus, and Amblyomma lepidum. Hyalomma dromedarii (94.3%) was the most abundant species, followed by H. anatolicum (32.8%). Camels were heavily infested (94%) with ticks as compared to cows (38%), sheep (37%), and goats (14%). Widespread occurrence of these four tick species in the UAE poses a risk of spreading tick-borne pathogens wherever the conditions of infection prevail.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Prevalence, Distribution, and Molecular Record of Four Hard Ticks from Livestock in the United Arab Emirates

Perveen

Muzaffar

Al-Deeb

2021

Insects

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“…In recent years, several DNA markers such as the internal transcribed spacer (ITS) region, the cytochrome c oxidase subunit I (COI) gene, and the 16S rRNA gene have been used for tick species identification ( 15 , 52 , 56 , 57 ). However, despite their wide use, these markers have been found to have limitations in terms of their discriminatory power and sensitivity ( 15 , 27 , 58 , 59 ). For example, the ITS region has been shown to have limited resolution in differentiating between closely related species ( 27 ), while the COI gene has been found to have low sensitivity in detecting intraspecific genetic variations ( 27 ).…”

Section: Discussionmentioning

confidence: 99%

Development and field evaluation of PCR assays based on minimum length Bm86 cDNA fragments required for Rhipicephalus and Hyalomma tick species delineation

et al. 2023

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IntroductionHyalomma and Rhipicephalus ticks are important genera that can transmit diseases to both animals and humans, including Crimean-Congo hemorrhagic fever, tick-borne encephalitis, and several types of spotted fever. The accurate identification of tick species is essential for the effective control and prevention of tick-borne diseases. However, traditional identification methods based on morphology can be challenging and subjective, leading to errors. The development of DNA markers has provided more precise and efficient methods for tick species identification, but the currently available markers have limitations in their discriminatory power and sensitivity. To address this need for more sensitive and specific markers, this study aimed to identify two minimum sequence fragments required for tick Hyalomma and Rhipicephalus species identification using the Bm86 cDNA marker, which has previously been shown to be in perfect agreement with the current taxonomy of hard ticks based on its complete sequence.MethodsBased on our in silico determination that a minimum sequence of 398 bp for Rhipicephalus spp. (from 1487 to 1884) and 559 bp for Hyalomma species (from 539 to 1097) was necessary for species delineation, two distinct PCR assays were developed to apply these sequences in practice.Results and discussionDiscrimination between species within each genus was achieved through sequence homology and phylogenetic analysis following the sequencing of the two PCR products. Subsequently, their performance was evaluated by testing them on the field-collected ticks of the Hyalomma and Rhipicephalus genera obtained from various host animals in different geographic regions of Tunisia. The use of shorter partial sequences specific to the tick genera Rhipicephalus and Hyalomma, which target the tick's RNA banks, could represent a significant advance in the field of tick species identification, providing a sensitive and discriminatory tool for interspecific and intraspecific diversity analysis.

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“…RAPD-PCR also allows analysis of the genetic diversity of tick species. Recently, this method was used by Al-Deeb and Enan who studied Hyalomma dromedarii, which does not appear in Europe,utilizing 13 decamer primers [55]. The RAPD-PCR technique is closely correlated with the operation of molecular markers MAAP (Multiple Arbitrary Amplicon Profiling) termed a genomic fingerprint.…”

Section: Pcr Reaction-based Methodsmentioning

confidence: 99%

Current Methods Used to Identify and Genotype Spirochaetes Borreliella Burgdorferi

Teodorowicz¹,

Weiner²

2020

hpc

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Authors' contribution Wkład autorów: A. Study design/planning zaplanowanie badań B. Data collection/entry zebranie danych C. Data analysis/statistics dane-analiza i statystyki D. Data interpretation interpretacja danych E. Preparation of manuscript przygotowanie artykułu F. Literature analysis/search wyszukiwanie i analiza literatury G. Funds collection zebranie funduszy Summary Lyme disease, as one of tick-borne diseases, has been a current epidemiological problem in Poland and in the world for several years. The proportion of borreliosis infections caused by B. burgdorferi spirochaetes is increasing. Difficulties diagnosing this disease with conventional methods have led to growing molecular biology research aimed at developing improved diagnostic tools. Molecular biology methods include all techniques that allow the detection and analysis of nucleic acids. Among them there are methods based on PCR reaction, molecular typing methods (MLST), new generation sequencing (NGS). The current development of this field gives great research opportunities. With regard to B. burgdorferi spirochaete, molecular biology is used to: confirm Lyme borreliosis, identify and type Borreliella spirochaetes, detect them in tick vectors or intermediate hosts, as well as to identify co-infections between different Borreliella species and other tick-borne pathogens. They are meant to significantly improve diagnostic analyzes. This paper reviews the current methods used for the detection and identification of B. burgdorferi. Molecular mechanisms for the survival of spirochaetes in the host, infection vectors and clinical picture of Lyme disease were also discussed.

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Genetic Diversity in the Camel Tick <i>Hyalomma dromedarii</i> (Acari: Ixodidae) Based on Mitochondrial Cytochrome c Oxidase Subunit I (COI) and Randomly Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR)

Cited by 4 publications

References 19 publications

Prevalence, Distribution, and Molecular Record of Four Hard Ticks from Livestock in the United Arab Emirates

Prevalence, Distribution, and Molecular Record of Four Hard Ticks from Livestock in the United Arab Emirates

Development and field evaluation of PCR assays based on minimum length Bm86 cDNA fragments required for Rhipicephalus and Hyalomma tick species delineation

Current Methods Used to Identify and Genotype Spirochaetes Borreliella Burgdorferi

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