Genetic diversity of almonds (Prunus dulcis) using RAPD technique

Amplified fragment length polymorphism as a tool for molecular characterization of almond germplasm: genetic diversity among cultivated genotypes and related wild species of almond, and its relationships with agronomic traits

“…Similar studies have been performed on non-Iranian cultivars and wild species from Asian and Mediterranean countries, and Australian and North America states, using several types of markers including, isozymes (Arulsekar et al 1986;Hauagge et al 1987;Cerezo et al 1989;Viruel et al 1995); RFLPs (Viruel et al 1995), RAPDs (Resta et al 1998; Bartolozzi et al 1998;Mir Ali and Nabulsi 2003), SSRs (Cipriani et al 1999;Martı´nez-Go´mez et al 2003a;2003b;Mnejja et al 2004;Xu et al 2004;Xie et al 2006); ISSRs (Martins et al 2003) and AFLPs (Martins et al 2001). These studies find high levels of variation and heterozygosity.…”

Genetic diversity and phylogenetic relationships among Tunisian almond cultivars revealed by RAPD markers

“…Similar studies have been performed on non-Tunisian cultivars and wild species of almond from Asia, other Mediterranean countries, Australia, and North America using several types of markers, including isozymes (Arulsekar et al, 1986;Hauagge et al, 1987;Cerezo et al, 1989;Viruel et al, 1995), RFLPs (Viruel et al, 1995), ISSRs (Martins et al, 2003), and AFLPs (Martins et al, 2001). RAPDs have been used for almond genotyping, DNA fingerprinting, and molecular characterisation (Bartolozzi et al, 1998;Resta et al, 1998;Joobeur et al, 2000;Mir Ali and Nabulsi, 2003), and have also been used in many other species such as apricot [P. armenica L. (Gogorcena and Parfitt, 1994)], olive [Olea europea L. (Belaj et al, 2002)], banana [Musa spp. L. (Onguso et al, 2004)], fig [Ficus carica L. (De Masi et al, 2005)], plum [Prunus L. ssp.…”

“…Bartolozzi et al (1998) used 20 primers to study genetic diversity among 17 Californian almond cultivars, but was unable to distinguish between them, and therefore proposed the use of more primers. Mir Ali and Nabulsi (2003) used 19 almond cultivars and indicated that 20 primers may be sufficient in the case of limited resources, although the use of 30 primers gave better resolution of the genetic relationships between cultivars. Our work demonstrated that the 12 selected primers permitted discrimination between all 58 genotypes.…”

Assessment of Genetic Diversity of Moroccan Cultivated Almond (<i>Prunus dulcis</i> Mill. DA Webb) in Its Area of Extreme Diffusion, Using Nuclear Microsatellites

“…This slight decrease in diversity towards the West of the Mediterranean is consistent with studies previously established on the species. Using the RAPD method, Mir Ali and Nabulsi [63], found that genetic diversity of Syrian almonds exceeds that found by Bartolozzi et al [31] who reported a low variability in RAPD markers among California almond cultivars. In the same direction, Martins et al [32,33] found considerable polymorphism in the Portuguese almond collection.…”