Genetic diversity of Anaplasma marginale strains from an outbreak of bovine anaplasmosis in an endemic area

Alamzán, Consuelo; Medrano, Citlaly; Ortíz, Martín; Fuente, José de la

doi:10.1016/j.vetpar.2008.08.015

Cited by 35 publications

(22 citation statements)

References 21 publications

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“…In addition, for the isolates described from South America, the most common tandem repeats in the first repetition are 4, 16, 72, α and τ in Brazil (VIDOTTO et al, 2006;POHL et al, 2013;SILVA et al, 2015a, b) and α, τ and B in Argentina (RUYBAL et al, 2009). Previous studies have associated the presence of the strain α-β 3 -Γ with the occurrence of clinical anaplasmosis in Mexico (ALMAZÁN et al, 2008) and Brazil (SILVA et al, 2015b); however, the present study is the first that associates the occurrence of the τ-10-15 strain with animal mortality. Thus, we believe that the A. marginale strains τ-10-15 and α-β 3 -Γ may be involved in the occurrence of bovine anaplasmosis outbreaks in endemic areas.…”

Section: Discussionmentioning

confidence: 97%

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Outbreak of anaplasmosis associated with the presence of different Anaplasma marginale strains in dairy cattle in the states of São Paulo and Goiás, Brazil

Machado

Silva

André

et al. 2015

Rev. Bras. Parasitol. Vet.

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The present study reports the genetic diversity of Anaplasma marginale during anaplasmosis outbreaks in rural properties of the states of Goiás and São Paulo, Brazil. Mortality rates of 3.5% (37/1,050) in calves, 4.7% (45/954) in heifers and 1.1% (25/2,200) in lactating cows were observed in a cattle herd of the municipality of Mambaí, state of Goiás, central-western Brazil. In a cattle herd from the municipality of Lins, state of São Paulo, in southeastern Brazil, none of the animals died, despite presenting clinical signs suggestive of bovine anaplasmosis and exhibiting a drastic decrease in milk production. Thus, blood samples were collected from 100 animals with clinical signs suggestive of bovine anaplasmosis in the municipalities of Mambaí and Lins. Based on the microsatellite structure of the MSP1a of A. marginale, the genotypes E and H were observed in Lins, and the C, D and E genotypes were found in Mambaí. The analysis of the tandem repeat structures of the MSP1a showed nine different strains (τ-10 -15, α-β 2 , α-β 3 -13, α-β 2 192, τ-β-100, α-β 2 -Γ, 193-β-100, 191-13-Γ and 191-13-18) in Lins and two (α-β 3 -Γ and E-F-φ 2 -F 2 ) in Mambaí. Three new tandem repeats of MSP1a (191, 192 and 193) were described. The τ-10-15 and α-β 3 -Γ strains were predominantly associated with the occurrence of clinical anaplasmosis and mortality in calves, heifers and lactating cows.

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Section: Discussionmentioning

confidence: 97%

“…The α-β 3 -Γ and τ-10-15 strains have been previously described in outbreaks of bovine anaplasmosis in Mexico (ALMAZÁN et al, 2008) and Argentina (RUYBAL et al, 2009). In addition, the 72-62-61 strain has been reported as the most common in a cattle herd from the state of Minas Gerais, Brazil (POHL et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Outbreak of anaplasmosis associated with the presence of different Anaplasma marginale strains in dairy cattle in the states of São Paulo and Goiás, Brazil

Machado

Silva

André

et al. 2015

Rev. Bras. Parasitol. Vet.

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“…The authors also described three new repeats of MSP1a (191, 192, and 193) and associated the τ -10-15 and α - β 3 -Γ strains with the occurrence of clinical anaplasmosis and mortality in calves, heifers, and lactating cows. As reported before, out of the different strains of A. marginale identified worldwide some have been associated with the occurrence of anaplasmosis outbreaks; specifically, the α , β , Γ and τ , 10, 15 strains have been previously described in outbreaks of bovine anaplasmosis in Mexico and Argentina [40, 41]. …”

Section: Diversity Of Anaplasma Marginalementioning

confidence: 99%

“…Through a reconstruction of ancestral amino acid sequence, they found that tandem 4 is the ancestral state of all new repeats reported in South Africa. It should be interesting to test whether the tandem repeat 4 from MSP1a reported in Mexican A. marginale strains evolved from the South African repeat [41]. The authors suggest that the repeated sequences identified in South Africa may constitute a group of recently evolved tandem repeats, which have not been reported elsewhere [27].…”

Section: Diversity Of Anaplasma Marginalementioning

confidence: 99%

Anaplasma marginale: Diversity, Virulence, and Vaccine Landscape through a Genomics Approach

Quiroz-Castañeda

Amaro-Estrada

Rodríguez-Camarillo

2016

BioMed Research International

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In order to understand the genetic diversity of A. marginale, several efforts have been made around the world. This rickettsia affects a significant number of ruminants, causing bovine anaplasmosis, so the interest in its virulence and how it is transmitted have drawn interest not only from a molecular point of view but also, recently, some genomics research have been performed to elucidate genes and proteins with potential as antigens. Unfortunately, so far, we still do not have a recombinant anaplasmosis vaccine. In this review, we present a landscape of the multiple approaches carried out from the genomic perspective to generate valuable information that could be used in a holistic way to finally develop an anaplasmosis vaccine. These approaches include the analysis of the genetic diversity of A. marginale and how this affects control measures for the disease. Anaplasmosis vaccine development is also reviewed from the conventional vaccinomics to genome-base vaccinology approach based on proteomics, metabolomics, and transcriptomics analyses reported. The use of these new omics approaches will undoubtedly reveal new targets of interest in the near future, comprising information of potential antigens and the immunogenic effect of A. marginale proteins.

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“…Conversely, highly specialized susceptible cattle from low endemicity areas are subject to great risk when introduced to high endemicity or places where tick populations fluctuate during the year. This phenomenon has been clearly verified in Mexico, where up to 30% of susceptible cattle have died in outbreaks of anaplasmosis (ALMAzÁN et al, 2008).…”

Section: Seroprevalence and Risk Factorsmentioning

confidence: 75%

Longitudinal study of risk factors for anaplasmosis and transplacental transmission in herd cattle

2014

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Anaplasma marginale, bacteria that occurs in tropical and subtropical areas throughout the world, is responsible for causing a disease that is one of the most constraint to cattle production in many countries. The present work aimed to investigate potential risk factors for anaplasmosis and the occurrence of transplacental transmission by molecular and serological assays in cattle. A total of 22 calves were randomly sampled and monitored during four years; the presence of infection/exposure to A. marginale was assessed by a semi-nested PCR assay targeting msp-5 gene of A. marginale, indirect ELISA for detection of IgG antibodies to A. marginale and blood smears. The A. marginale infection prevalence data were analyzed in relation to risk factors, using chi-squared, odds ratio and multiple logistic regressions. DNA amplification results revealed a frequency of infection with A. marginale of 41% (9/22) in the newborn calves before colostrum suckling. However, only 14% (3/22) of newborn calves were seropositive for A. marginale. All calves were positive to A. marginale by PCR and microscopic examination of blood smears before the two and fourth month of age, respectively. The main risk factors associated with seroprevalence were breed (OR=36.2), tick infestation (OR=3.44) and stocking density (OR=3.28). The results indicated that exposure of cattle to A. marginale was common in dairy herds and endemic instability situation probably is due to inadequate antibody production in cows or genetic variability of pathogen. This study still demonstrated that in addition to transmission of A. marginale by ticks and flies, the transplacental transmission is very important and should be the target of disease control programs in Brazil. Key words: Anaplasma marginale, blood smear, ELISA, PCR ResumoAnaplasma marginale, bactéria que ocorre em regiões tropicais e subtropicais do mundo, é responsável por causar uma das doenças que mais restringem a criação de bovinos em diversos países. O presente trabalho teve como objetivo investigar potenciais fatores de risco para a anaplasmose e a ocorrência de transmissão transplacentária por ensaios moleculares e sorológicos em bovinos. (OR = 3,28). Os resultados indicaram que a exposição de gado para A. marginale era comum nos rebanhos leiteiros e a situação de instabilidade endêmica provavelmente se faz devido à produção inadequada de anticorpos em vacas ou a variabilidade genética do patógeno. Este estudo demonstra ainda que, além da transmissão de A. marginale por carrapatos e moscas, a transmissão transplacentária é muito importante e deve ser alvo de programas de controle da doença no Brasil.

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Genetic diversity of Anaplasma marginale strains from an outbreak of bovine anaplasmosis in an endemic area

Cited by 35 publications

References 21 publications

Outbreak of anaplasmosis associated with the presence of different Anaplasma marginale strains in dairy cattle in the states of São Paulo and Goiás, Brazil

Outbreak of anaplasmosis associated with the presence of different Anaplasma marginale strains in dairy cattle in the states of São Paulo and Goiás, Brazil

Anaplasma marginale: Diversity, Virulence, and Vaccine Landscape through a Genomics Approach

Longitudinal study of risk factors for anaplasmosis and transplacental transmission in herd cattle

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