Genetic diversity of Aquilegia (Ranunculaceae) species and cultivars assessed by AFLPs

Zhu, Ruirui; Gao, Yin; Xu, Liang; Zhang, Q.X.

doi:10.4238/vol10-2gmr1112

Cited by 9 publications

(10 citation statements)

References 20 publications

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“…The cluster analysis showed that cultivars from the same geographic regions tended to group together (Zhu et al, 2011). Cultivars from North America mainly grouped in Clusters A and B, while Chinese cultivars grouped in Cluster C. There was an interesting exception in that Nos.…”

Section: From 50mentioning

confidence: 92%

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Genetic diversity of Lagerstroemia (Lythraceae) species assessed by simple sequence repeat markers

Liu²,

Cai³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Lagerstroemia (crape myrtle) are famous ornamental plants with large pyramidal racemes, long flower duration, and diverse colors. However, little is known about the genetic structure and diversity of germplasm in Lagerstroemia. We genotyped 81 L. indica cultivars, five other species of Lagerstroemia, and 10 interspecific hybrids using 30 simple sequence repeat markers; 275 alleles were generated with a mean of nine alleles per locus. The mean polymorphism information content value, a measure of gene diversity, was 0.63, with a range from 0.25 to 0.86. The mean observed heterozygosity (0.51) tended to be lower than the mean expected heterozygosity (0.67). The mean F-statistics (F ST , F IS , and F IT ) were 0.05, 0.20, and 0.24, respectively, indicating a high level of genetic variation among cultivars. Clustering analysis based on genetic distance divided the 96 genotypes into three distinct groups, which corresponded with their genetic backgrounds and geographic regions. L. indica cultivars and the other five L. species were grouped into different sub-clusters. Chinese and North American cultivars were divided into different clusters. These data about the genetic relationship among cultivars demonstrated the potential value of L. indica cultivars and other Lagerstroemia species for widening the genetic basis of breeding programs for this ornamental flower.

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Section: From 50mentioning

confidence: 92%

“…DNA concentrations were estimated with Smart Ladder (TianGen) on a 1% (w/v) agarose gel containing Gel Red at 0.1 μg/mL 1X TAE buffer, with bands visualized under UV light (Zhu et al, 2011 …”

Section: Dna Extractionmentioning

confidence: 99%

Genetic diversity of Lagerstroemia (Lythraceae) species assessed by simple sequence repeat markers

Liu²,

Cai³

et al. 2012

Genet. Mol. Res.

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“…DNA concentrations were estimated with Smart Ladder (TianGen) on a 1% (w/v) agarose gel containing Gel Red at 0.1 μg/ml in 1× TAE buffer, with bands visualized under UV light (Zhu et al. ).…”

Section: Methodsmentioning

confidence: 99%

The first genetic linkage map of crape myrtle (Lagerstroemia) based on amplification fragment length polymorphisms and simple sequence repeats markers

Liu

Cai

et al. 2013

Plant Breeding

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Lagerstroemia (crape myrtle) are famous ornamental plants with large pyramidal racemes, long flower duration and diverse colours. Genetic maps provide an important genomic resource of basic and applied significance. A genetic linkage map was developed by genotyping 192 F1 progeny from a cross between L. caudata (female) and L. indica (‘Xiang Xue Yun’) (male) with a combination of amplification fragment length polymorphisms (AFLP) and simple sequence repeats (SSR) markers in a double pseudo‐testcross mapping strategy. A total of 330 polymorphic loci consisting of 284 AFLPs and 46 SSRs showing Mendelian segregation were generated from 383 AFLP primer combinations and 150 SSR primers. The data were analysed using JoinMap 4.0 (evaluation version) to construct the linkage map. The map consisted of 20 linkage groups of 173 loci (160 AFLPs and 13 SSRs) covering 1162.1 cM with a mean distance of 10.69 cM between adjacent markers. The 20 linkage groups contained 2–49 loci and ranged in length from 7.38 to 163.57 cM. This map will serve as a framework for mapping QTLs and provide reference information for future molecular breeding work.

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“…DNA quality and concentration were measured by 1% agarose gel electrophoresis containing Gel Red at 0.1 mg/mL 1X TAE buffer, with bands visualized under UV light (Zhu et al, 2011).…”

Section: Dna Extractionmentioning

confidence: 99%

Screening of molecular markers linked to dwarf trait in crape myrtle by bulked segregant analysis

Ye¹,

Liu²,

Cai³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Plant height is one of the most important traits of plant architecture as it modulates both economic and ornamental values. Crape myrtle (Lagerstroemia indica L.) is a popular ornamental woody plant because of its long-lasting mid-summer bloom, rich colors, and diversified plant architecture. These traits also make it an ideal model of woody species for genetic analysis of many ornamental traits. To understand the inheritance of plant height and screen for genes modulating plant height in Lagerstroemia, segregation of the plant height trait was analyzed using the F 1 population of L. fauriei (standard) x L. indica 'Pocomoke' (dwarf) with 96 seedlings, while dwarf genes were screened using the bulked segregant analysis method, combined with 28 amplified fragment length polymorphism primers and 41 simple sequence repeat primers. The results showed that the dwarf trait of crape myrtle was controlled by a major gene and modified by minor genes. An amplified fragment length polymorphism marker, M53E39-92, which was 23.33 cM from the loci controlling the dwarf trait, was screened. These results provide basic information for marker-assisted selection in Lagerstromia and cloning of dwarf genes in future studies.

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Genetic diversity of Aquilegia (Ranunculaceae) species and cultivars assessed by AFLPs

Cited by 9 publications

References 20 publications

Genetic diversity of Lagerstroemia (Lythraceae) species assessed by simple sequence repeat markers

Genetic diversity of Lagerstroemia (Lythraceae) species assessed by simple sequence repeat markers

The first genetic linkage map of crape myrtle (Lagerstroemia) based on amplification fragment length polymorphisms and simple sequence repeats markers

Screening of molecular markers linked to dwarf trait in crape myrtle by bulked segregant analysis

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