Genetic diversity of Astragalus nitidiflorus, a critically endangered endemic of SE Spain, and implications for its conservation

Vicente, María José; Segura, Francisco; Aguado, M.T.; Migliaro, Daniele; Franco, J.A.; Martínez-Sánchez, J.J.

doi:10.1016/j.bse.2011.03.005

Cited by 40 publications

(27 citation statements)

References 24 publications

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“…On the other hand, the mean number of bands per primer attained in the present study was higher than those generated with V. unguiculata (8.6 bands/ primer; Ghalmi et al, 2010) and V. amoena (8.1 bands/primer; Liu et al, 2013), while the amplicons derived from C. argentea were larger than those obtained from Astragalus nitidiflorus (300-1500 bp; Vicente et al, 2011) and Derris trifoliata (250-2000 bp; Wu et al, 2012).…”

Section: Discussioncontrasting

confidence: 43%

Molecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers

Luz¹,

Gomes²,

Neto³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Cratylia argentea (Desv.) Kuntze (Fabaceae) is a droughttolerant, perennial legume found primarily in Brazil, Bolivia, and Peru. The shrub is well adapted to acid soils and exhibits high productivity and nutritional value, characteristics that would favor its use as a dry season animal forage supplement in semiarid regions. In plant improvement programs, the production of elite hybrids with superior traits is generally achieved by crossing parents that exhibit the highest level of genetic divergence. Therefore, the aim of the present study was to assess genetic diversity among 13 accessions of C. argentea from the same population maintained in the active germplasm bank of Embrapa Meio-Norte using inter-simple sequence repeat (ISSR) markers. Genetic similarities between C. argentea accessions were estimated from Jaccard coefficients, and a dendrogram was constructed using the unweighted pair group method with arithmetic average (UPGMA). The set of 15 primers selected for ISSR analysis generated a total of 313 loci of which 79.23% were polymorphic. The mean number of bands per primer was 20.87, and the amplicons 15243 Genetic diversity among accessions of Cratylia argentea ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 15242-15248 (2015) ranged from 280 to 3000 bp in size. Primers UBC834 and UBC827 generated the largest number of polymorphic loci and exhibited 90.91 and 100% polymorphism, respectively. The coefficients of genetic similarity among accessions varied between 0.49 and 0.73. UPGMA cluster analysis allowed the identification of four genotypic groups and demonstrated the existence of considerable variability within the collection. Potential progenitors were selected that would offer good possibilities of obtaining unusual and favorable combinations of genes in a plant breeding program.

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Section: Discussioncontrasting

confidence: 43%

Molecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers

Luz¹,

Gomes²,

Neto³

et al. 2015

Genet. Mol. Res.

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“…Consequently, ISSR markers represent a powerful tool for investigating genetic variation within a species (Culley and Wolfe, 2001;Vicente et al, 2011;Rubio-Moraga et al, 2012). ISSR markers have been widely used for investigating population genetics in various plant species (Gajera et al, 2010;Wang et al, 2012;Zhang et al, 2013;Chen et al, 2013;Lin and Hu, 2013).…”

Section: Introductionmentioning

confidence: 99%

Analysis of the genetic diversity of Chinese native Cannabis sativa cultivars by using ISSR and chromosome markers

Zhang¹,

Chang²,

Zhang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Hemp (Cannabis sativa) is an important fiber crop, and native cultivars exist widely throughout China. In the present study, we analyzed the genetic diversity of 27 important Chinese native hemp cultivars, by using inter-simple sequence repeats (ISSR) and chromosome markers. We determined the following chromosome formulas: 2n = 20 = 14m + 6sm; 2n = 20 = 20m; 2n = 20 = 18m + 2sm; 2n = 20 = 16m + 4sm; and 2n = 20 = 12m + 8sm. The results of our ISSR analysis revealed the genetic relationships among the 27 cultivars; these relationships were analyzed by using the unweighted pair-group method based on DNA polymorphism. Our results revealed that all of the native cultivars showed considerable genetic diversity. At a genetic distance of 0.324, the 27 varieties could be classified into five categories; this grouping corresponded well with the chromosome formulas. All of the investigated hemp cultivars represent relatively primitive types; moreover, the genetic distances show a geographical distribution, with a small amount of regional hybridity.

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“…Так, для редкого вида Astragalus crassicarpus var. trichocalus генетическая дистанция со-ставила 0.429-0.715 (Rogenski et al, 2009), для редкого эндемичного вида Astragalus nitidiflorus диффиренциация между популяциями равна 0.279 (Vicente et al, 2011), у редких видов Astragalus olchonensis Gontsch. и Astraga lus sericeocanus Gontsch.…”

Section: результаты и обсуждениеunclassified

Variability and interpopulation differentiation of the rare species Gueldenstaedtia monophylla Fisch. (Fabaceae)

Selyutina¹,

Konichenko²,

Дорогина³

2017

Vestn. VOGiS

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central Siberian Botanical garden SB raS, novosibirsk, russia genetic variability of the rare species Guelden staedtia monophylla from 7 natural populations in the central part of its range (ongudai district, altai republic) was studied. to characterize the genetic diversity of this rare relict species at the population level, SDS-electrophoresis of seed storage proteins was used. Polypeptide spectra of seeds contained from 17 to 32 protein components, of which 28 were polymorphic. the populations of G. mono phylla were revealed to have a sufficiently high level of genetic polymorphism, the genetic similarity index within the populations studied ranged from 0.673 to 0.813. the highest variability of seed storage proteins was found in the populations inegen (0.673) and Malaya inya (0.734). the lowest level of variability of the polypeptide spectra was in the population Bol'shoy Yaloman (0.813). the nei genetic distance between the populations studied was 0.018-0.215, the greatest distance in the protein spectra was found between inegen and Malaya inya (0.215). inegen was the most remote from the other populations, the nei distance between this population and all other populations varying from 0.113 to 0.215. With aMoVa, it was found that the share of intra-population variability is 53 % and inter-population, 47 %. Perhaps this high genetic diversity in populations of G. monophylla is provided and maintained by such biological characteristics of the species as cross-pollination, high life expectancy and a long reproductive period. the results of our study suggest that some rare species are able to maintain high levels of genetic diversity, even in a small-size population. the relatively high level of genetic variability indicates that the current genetic drift and inbreeding do not pose a threat to the survival of the species.Key words: genetic diversity; rare species; Gueldenstaedtia monophylla; SDS-electrophoresis; seed storage proteins polymorphism. в работе изучена генетическая изменчивость редкого вида гюльден-штедтии однолистной (Gueldenstaedtia monophylla) из семи природных популяций в центральной части ее ареала (Онгудайский район респуб-лики алтай). для характеристики генетического разнообразия этого реликтового вида как на видовом, так и на популяционном уровне был использован SDS-электрофорез запасных белков семян. Полипеп-тидные спектры семян содержали от 17 до 32 белковых компонентов, из которых 28 были полиморфными. в популяциях G. monophylla вы-явлен достаточно высокий уровень генетического полиморфизма, ве-личина индекса генетического сходства внутри изученных популя ций варьировала от 0.673 до 0.813. наибольшая изменчивость запасных белков семян установлена в популяциях инегень (0.673) и Малая иня (0.734). самый низкий уровень изменчивости полипептидных спект-ров выявлен в популяции Большой Яломан (0.813). генетическая дис-танция nei между изученными популяциями составила 0.018-0.215, наибольшая дистанция по белковым спектрам (0.215) найдена между популяциями инегень и Малая иня. Популяция инегень...

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Genetic diversity of Astragalus nitidiflorus, a critically endangered endemic of SE Spain, and implications for its conservation

Cited by 40 publications

References 24 publications

Molecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers

Molecular characterization of accessions of Cratylia argentea (Camaratuba) using ISSR markers

Analysis of the genetic diversity of Chinese native Cannabis sativa cultivars by using ISSR and chromosome markers

Variability and interpopulation differentiation of the rare species Gueldenstaedtia monophylla Fisch. (Fabaceae)

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