Genetic Diversity of Barley Accessions from Ethiopia for Powdery Mildew Resistance

Абдуллаев, Р. А.; Lebedeva, T. V.; Yakovleva, O. V.; Ковалева, Ольга Николаевна; Radchenko, E. E.; Anisimova, I. N.; Batasheva, B. A.; Karabitsina, Yu. I.; Кузнецова, Е. Б.

doi:10.3103/s1068367419030029

Cited by 10 publications

(10 citation statements)

References 8 publications

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“…With the use of DNA markers, the 15 accessions were revealed which carried the mlo11 allele, ensuring the durable resistance of most modern barley varieties. The mildew resistance of the other 59 forms has been shown to be controlled by effective genes which are different from mlo11 (Abdullaev et al, 2019).…”

Section: Geneticsmentioning

confidence: 99%

“…Molecular marker techniques facilitated a more efficient work with collections of plant genetic resources. Molecular screening of accession collections of cereal (wheat, barley, sorghum) and oil (sunflower) crops is currently an integral part of plant genetic variability studies (Tyryshkin et al, 2006;Anisimova et al, 2011;Abdullaev et al, 2017Abdullaev et al, , 2019.…”

Section: Geneticsmentioning

confidence: 99%

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The ideas of N.I. Vavilov and current problems of crop genetics

Anisimova

Radchenko

2020

BioComm

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Some results of the studies of plant genetic resources held in the VIR Department of Genetics are discussed in light of N. I. Vavilov’s theories of natural plant immunity, homologous series in hereditary variability, and interspecific hybridization. The long-term investigations resulted in identifying bread wheat, cultivated barley, oat, rye and sorghum accessions with new effective genes for resistance to diseases and pests. The large-scale immunological screening has revealed a narrow genetic diversity in wheat and barley for resistance to biotic and abiotic stressors. On the example of sunflowers, the significance of seed storage proteins for solving problems of domestication and introgressive hybridizations is demonstrated.

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Section: Geneticsmentioning

confidence: 99%

Section: Geneticsmentioning

confidence: 99%

The ideas of N.I. Vavilov and current problems of crop genetics

Anisimova

Radchenko

2020

BioComm

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“…В наших исследованиях было показано, что 925 образцов ячменя из Эфиопии, сохраняемые в коллекции генетических ресурсов ВИР, различались по уровню устойчивости к B. graminis. (Abdullaev et al, 2019), а 200 образцов из Японии в полевых условиях также оказались неоднородными по устойчивости к патогену (неопубликованные данные).…”

Section: Introductionunclassified

Identification of barley accessions from the VIR collection carrying the <i>mlo11(cnv2)</i> powdery mildew resistance allele

Alpatieva

Lebedeva

Kovaleva

et al. 2021

Biotehnologiâ i selekciâ rastenij

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Background. The search for barley (Hordeum vulgare L.) genotypes that carry effective genes for resistance to powdery mildew agent Blumeria graminis f. sp. hordei is a present-day issue for Russian plant breeding. The mlo11 allele that confers long-term protection of barley against the pathogen is rarely found among the varieties, approved for cultivation in the territory of Russia. There is no information on the occurrence among Russian varieties of another effective allele, mlo11 (cnv2), therefore, the search for its source is a current necessity. Materials and methods. Seven barley accessions from Ethiopia and 7 accessions from Japan have been tested for resistance to the northwestern population of the powdery mildew agent in the field and in laboratory conditions. To identify of the Mlo gene alleles, nucleotide sequences of the Stowaway-MITE (Miniature Inverted-repeat Transposable Elements) and the adjacent promoter fragments were determined. Results. Phytopathological tests in the field and greenhouse conditions, as well as molecular markers were used to study 14 barley accessions from Ethiopia and Japan. According to the preliminary tests, plants were resistant to powdery mildew. The highly effective allele of powdery mildew resistance mlo11 (cnv2) was for the first time identified in four barley accessions from Ethiopia, k-20087, k-20523, k-20524 and k-28126. Under field conditions, adult plants were resistant, and in the greenhouse they were moderately damaged by powdery mildew (1-2 points). The disease symptoms were similar to those described for the sample Eth295, a carrier of the mlo11(cnv2) allele variant: single pustules and the absence of necrotic spots on the leaves. The fragments of Stowaway-MITE and adjacent Mlo 5' promoter sequences were amplified in all 14 accessions. The amplicons were cloned and sequenced. The unique marker SNPs within the MITE and Mlo 5’ promoter sequences, i.e. the substitutions of cytosine by thymine in positions 262 and 452, were found only in k-20087, k-20523, k-20524 and k-28126. These accessions belong to different botanical varieties and differ from each other in a number of morphological features, i.e. they are not duplicates. Conclusions. The genotypes selected as a result of the study can serve as a source of the mlo11(cnv2) allele in breeding powdery mildew-resistant barley varieties.

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“…выделили 27 устойчивых к B. graminis образцов, 47 форм были гетерогенны по изученному признаку. С помощью молекулярных маркеров выявили 15 образцов, несущих аллель mlo11, который обеспечивает длительную устойчивость к мучнистой росе большинства современных сортов ячменя [5].…”

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“…Все изученные формы устойчивы к мучнистой росе. Образцы к-23450 и к-29720 защищены геном устойчивости к грибу mlo11 [5], образец к-27670 имеет другой эффективный ген (гены) устойчивости к B. graminis. Выявленные нами формы представляют большой интерес для селекции в различных зонах России.…”

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Комплексная Устойчивость Образцов Ячменя Из Эфиопии К Биотическим И Абиотическим Стрессорам

2020

Генофонд И Селекция Растений: Доклады И Сообщения v Международной Конференции «Генофонд И Селекция Растений» (Новосибирск, 11–1

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Genetic Diversity of Barley Accessions from Ethiopia for Powdery Mildew Resistance

Cited by 10 publications

References 8 publications

The ideas of N.I. Vavilov and current problems of crop genetics

The ideas of N.I. Vavilov and current problems of crop genetics

Identification of barley accessions from the VIR collection carrying the <i>mlo11(cnv2)</i> powdery mildew resistance allele

Комплексная Устойчивость Образцов Ячменя Из Эфиопии К Биотическим И Абиотическим Стрессорам

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