Genetic diversity of BCoV in Brazilian cattle herds

Fernandes, Adeline de Mira; Brandão, Paulo Eduardo; Lima, Michele dos Santos; Martins, Maira de Souza Nunes; Silva, Thaís G. da; Pinto, Vivian da Silva Cardoso; Paula, Larissa T. de; Vicente, Marta Elisabete S.; Okuda, Líria Hiromi; Pituco, Edviges Maristela

doi:10.1002/vms3.102

Cited by 11 publications

(8 citation statements)

References 17 publications

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“…Furthermore, faecal-based serological studies have shown that BCoV is prevalent in cattle in Turkey [16,26,[28][29][30][31][32], while two studies have shown that is associated with nasal discharge in Turkey [29,33]. The prevalence of BCoV in our study is consistent with that reported previously in Turkey and in various other regions worldwide [5,[34][35][36][37][38][39][40].…”

Section: Discussionsupporting

confidence: 90%

Bovine coronavirus infections in Turkey: molecular analysis of the full-length spike gene sequences of viruses from digestive and respiratory infections

Temizkan

Alkan

2021

Arch Virol

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Bovine coronavirus (BCoV) can be spread by animal activity. Although cattle farming is widespread in Turkey, there are few studies of BCoV. The aim of this study was to evaluate the current situation regarding BCoV in Turkey. This is the first study reporting the full-length nucleotide sequences of BCoV spike (S) genes in Turkey. Samples were collected from 119 cattle with clinical signs of respiratory (n = 78) or digestive tract (n = 41) infection on different farms located across widely separated provinces in Turkey. The samples were screened for BCoV using RT-nested PCR targeting the N gene, which identified BCoV in 35 samples (9 faeces and 26 nasal discharge). RT-PCR analysis of the S gene produced partial/full-length S gene sequences from 11 samples (8 faeces and 3 nasal discharge samples). A phylogenetic tree of the S gene sequences was made to analyze the genetic relationships among BCoVs from Turkey and other countries. The results showed that the local strains present in faeces and nasal discharge samples had many different amino acid changes. Some of these changes were shown in previous studies to be critical for tropism. This study provides new data on BCoV in Turkey that will be valuable in designing effective vaccine approaches and control strategies.

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Section: Discussionsupporting

confidence: 90%

Bovine coronavirus infections in Turkey: molecular analysis of the full-length spike gene sequences of viruses from digestive and respiratory infections

Temizkan

Alkan

2021

Arch Virol

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“…The prevalence results of this study were found to be consistent with other studies conducted in Turkey. Also, the prevalence of the BCoV in divergent regions of the world is considerably similar with this study and Turkey [5,[34][35][36][37][38][39][40].…”

Section: Discussionsupporting

confidence: 87%

Bovine Coronavirus Infections in Turkey: Molecular Analysis of the Full-Length Spike Gene Sequences of Viruses From Digestive and Respiratory Infections

Temizkan

Alkan

2021

Preprint

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BCoV can be spread by animal activity. Despite the widespread cattle farming in the region, limited studies exist in Turkey about BCoV. The aim of this study was to reveal the latest aspects in Turkey about BCoV and evaluate the data. This is the first study reporting the full-length sequences of S genes of BCoV in Turkey.In this study samples were collected from 119 cattle, presenting clinical signs of respiratory (n=78) or digestive tract infection (n=41) in distinctive farms located in widely different provinces of Turkey. Samples were screened for bovine coronavirus (BCoV) by a RT-nested PCR targeting the N gene. BCoV was found positive in 35 samples (9 faeces and 26 nasal discharge) and RT-PCR analysis of the S gene was performed. The partial/full-length S gene sequences were able to be obtained from 11 samples (8 faeces and 3 nasal discharge). Phylogenetic tree of the S gene sequences was analyzed for the genetic relationships among BCoVs from Turkey and other countries. Results showed that many different amino acid changes exist in local strains and these changes were persistent in faeces and nasal discharge origin. Likewise, some nucleotide and amino acid changes were compatible with previous studies which have critical importance for tropism. These results could represent a novelty in the current literature adding new data on BCoV from Turkey and these data could have potentially positive effects on the vaccination approach and control strategies due to lack of information.

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“…The cattle industry is one of the main sources of income in Uruguay, and this country is one of the main exporters of meat and dairy products worldwide [7][8][9]. In this work, we demonstrate the circulation of BCoV in calves in Uruguay, with a frequency of 7.8%, which is higher than in Argentina (1.71%) [18] but lower than in Brazil (14.9 -33.3%) [17,27,28].…”

Section: Discussionmentioning

confidence: 61%

Bovine coronavirus in Uruguay: genetic diversity, risk factors and transboundary introductions from neighboring countries

et al. 2019

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Bovine coronavirus (BCoV) is a recognized cause of severe neonatal calf diarrhea, with a negative impact on animal welfare, leading to economic losses to the livestock industry. Cattle production is one of the most important economic sectors in Uruguay. The aim of this study was to determine the frequency of BCoV infections and their genetic diversity in Uruguayan calves and to describe the evolutionary history of the virus in South America. The overall detection rate of BCoV in Uruguay was 7.8% (64/824): 7.7% (60/782) in dairy cattle and 9.5% (4/42) in beef cattle. The detection rate of BCoV in samples from deceased and live calves was 10.0% (6/60) and 7.6% (58/763), respectively. Interestingly, there was a lower frequency of BCoV detection in calves born to vaccinated dams (3.3%, 8/240) than in calves born to unvaccinated dams (12.2%, 32/263) (OR: 4.02, 95%CI: 1.81-8.90; p = 0.00026). The frequency of BCoV detection was higher in colder months (11.8%, 44/373) than in warmer months (1.5%, 3/206) (OR: 9.05, 95%CI: 2.77-29.53, p = 0.000013). Uruguayan strains grouped together in two different lineages: one with Argentinean strains and the other with Brazilian strains. Both BCoV lineages were estimated to have entered Uruguay in 2013: one of them from Brazil (95%HPD interval: 2011 and the other from Argentina (95%HPD interval: 2010-2014). The lineages differed by four amino acid changes, and both were divergent from the Mebus reference strain. Surveillance should be maintained to detect possible emerging strains that can clearly diverge at the antigenic level from vaccine strains.

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Genetic diversity of BCoV in Brazilian cattle herds

Cited by 11 publications

References 17 publications

Bovine coronavirus infections in Turkey: molecular analysis of the full-length spike gene sequences of viruses from digestive and respiratory infections

Bovine coronavirus infections in Turkey: molecular analysis of the full-length spike gene sequences of viruses from digestive and respiratory infections

Bovine Coronavirus Infections in Turkey: Molecular Analysis of the Full-Length Spike Gene Sequences of Viruses From Digestive and Respiratory Infections

Bovine coronavirus in Uruguay: genetic diversity, risk factors and transboundary introductions from neighboring countries

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