Genetic diversity of common bean (Phaseolus vulgaris L.) breeding collection in Serbia

Abstract

The characterization of 41 common bean cultivars and landraces from breeding collection of Institute of Field and Vegetable Crops, Novi Sad, Serbia, was done based on phenotypic traits and microsatellite markers. Phenotypic traits were chosen from Bioversity International descriptor list. In addition, main yield components were investigated. Analysis of phaseolin type revealed affiliation of cultivars and landraces to Mesoamerican or Andean gene pool. Cultivars and landraces demonstrated significant diversity level with regard to studied phenotypic traits. Identified variation showed high potential for developing new cultivars with desirable combination of traits. Principal component analysis based on phenotypic traits separated bean cultivars and landraces in two groups, which corresponded to Mesoamerican and Andean determined according to phaseolin type. Putative hybrids, with combination of traits between gene pools were also identified. Analysis of microsatellite data, using twenty-two SSR primer pairs, showed medium gene diversity in studied material. Microsatellite-based cluster analysis separated genotypes in two discrete clusters and several subclusters. No clear separation according to gene pool was found between the clusters, however grouping according to gene pool and patterns of phenotypic variation, following these gene pools, were observed within subclusters. Knowledge on detailed relationships of cultivars and landraces based on phenotypic and molecular data would facilitate identification of candidates for future breeding.

Morpho-Agronomic Characterisation of Runner Bean (Phaseolus coccineus L.) from South-Eastern Europe

“…Morpho-agronomic information in this study will help to promote runner-bean breeding for the development of new varieties with favourable traits. To date, only limited information is available on runner-bean accessions from South-Eastern Europe [14,37,39], as compared to the common bean [14,[35][36][37][38]40]. As a further step, analysis of this diversity using molecular approaches is recommended, to provide more information on the overall genetic diversity, and potentially on the genes responsible for the specific morpho-agronomic traits in this runner-bean collection from South-Eastern Europe.…”

Phylogeny and diversity of lamiaceae based on rps14 gene in Pakistan

“…As the significance of biodiversity is increasing day by day in the field of agriculture, evolutionary biology, ecology, conservation biology and other fields, therefore correct identification of plant taxa is very necessary, while DNA barcode is an important tool for the modern-day plant identification and delimitation (BAYAT et al, 2018;GALOVIĆ et al, 2018;WANG et al, 2018). Recent advances in the field of genomics and bioinformatics are incomplete without the use of DNA sequences (SEVĠNDĠK and YALÇIN, 2018;SAVIĆ et al, 2019). DNA sequences have become a major source of collecting information.…”

Genetic diversity and relationship of Tunisian castor (Ricinus communis L.) genotypes revealed by SSR markers

“…Random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers on the other hand, require only small amounts of DNA sample without involving radioactive labels and are simpler as well as faster (KAPTEYN and SIMON, 2002;JAN et al, 2011). SSR has proven to be quite efficient in detecting genetic variations and used for diversity assessment and for identifying germplasm in a number of species (MIKIĆ et al, 2018;NIKOLIĆ et al, 2018;SALEHI et al, 2018;RAJA et al, 2018;DARESTANI et al, 2019;ROGIĆ et al, 2019;SAVIĆ et al, 2019). The polymerase chain reaction (PCR), which is the basic technique of SSR markers, has been used by many authors, such as LABAJOVÁ et al, (2011);ŽIAROVSKÁ et al, (2013); ŠTEFÚNOVÁ et al, (2014); ŽIAROVSKÁ et al, (2014); KALLAMADIA et al, (2015); SHARAWY et al, (2015); VYHNÁNEK et.…”