Genetic diversity of common Gasterophilus spp. from distinct habitats in China

Zhang, Boru; Huang, Heqing; Wang, Haoyu; Zhao, Dong; Chu, Hongjun; Ma, Xinping; Yan, Guiqin; Ente, Make; Li, Kai

doi:10.1186/s13071-018-3042-y

Cited by 9 publications

(4 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The larvae absorb nutrients from the host and secrete toxins, and may lead to host death when the level of infection is severe [ 6 , 7 ]. The genus Gasterophilus comprises 9 species globally [ 8 , 9 ], of which 6 have been recorded in China [ 10 ]. The infection rate of bot flies in the equid population is 100% in Kalamaili Nature Reserve (KNR), Xinjiang, which is located in desert grassland [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

The impact of temperature on the life cycle of Gasterophilus pecorum in northwest China

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background The departure of the mature larvae of the horse stomach bot fly from the host indicates the beginning of a new infection period. Gasterophilus pecorum is the dominant bot fly species in the desert steppe of the Kalamaili Nature Reserve (KNR) of northwest China as a result of its particular biological characteristics. The population dynamics of G. pecorum were studied to elucidate the population development of this species in the arid desert steppe. Methods Larvae in the freshly excreted feces of tracked Przewalski’s horses (Equus przewalskii) were collected and recorded. The larval pupation experiments were carried out under natural conditions. Results There was a positive correlation between the survival rate and the number of larvae expelled (r = 0.630, p < 0.01); the correlation indicated that the species had characteristic peaks of occurrence. The main periods during which mature larvae were expelled in the feces were from early April to early May (peak I) and from mid-August to early September (peak II); the larval population curve showed a sudden increase and gradual decrease at both peaks. Under the higher temperatures of peak II, the adults developing from the larvae had a higher survival rate, higher pupation rate, higher emergence rate and shorter eclosion period than those developing from peak I larvae. Although G. pecorum has only one generation per year, its occurrence peaked twice annually, i.e. the studied population has a bimodal distribution, which doubles parasitic pressure on the local host. This phenomenon is very rarely recorded in studies on insect life history, and especially in those on parasite epidemiology. Conclusion The period during which G. pecorum larvae are naturally expelled from the host exceeds 7 months in KNR, which indicates that there is potentially a long period during which hosts can become infected with this parasite. The phenomenon of two annual peaks of larvae expelled in feces is important as it provides one explanation for the high rate of equine myiasis in KNR.

show abstract

Section: Introductionmentioning

confidence: 99%

The impact of temperature on the life cycle of Gasterophilus pecorum in northwest China

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Among the second-generation high-throughput sequencing technologies, Illumina de novo transcriptome sequencing has been applied to the study of various Diptera insects, including Calliphora stygia (Olivia et al, 2015), Episyrphus balteatus (Bin et al 2017), andBactrocera minax (Wu et al, 2019). However, in case of G. nasalis, most studies have focused on morphology, behavior, epidemiology, and population genetics (Leite et al 1999;Li et al 2018;Liu et al 2018;Wang et al 2016;Zhang et al 2018). Research on functional characterization of genes remain scarce.…”

Section: Introductionmentioning

confidence: 99%

De novo assembly and SSR loci analysis in Gasterophilus nasalis (Diptera: Oestridae)

Zhang

Zhou

et al. 2021

Entomological Research

Self Cite

View full text Add to dashboard Cite

Gasterophilus nasalis, an obligate parasite of equids, is distributed worldwide and causes severe damage when abundant in the host's digestive tract. However, detailed genomic information is lacking for this pathogen. In this study, we generated 27.47 Gb of clean data on G. nasalis using the Illumina Hiseq ™ 4000 sequencing platform. De novo analysis of sequencing reads revealed 37,289 unigenes obtained from G. nasalis, with an average length of 1,335 bp and N50 length of 1,808 bp. All unigenes were searched against the non-redundant (NR) databases using BlastX and were primarily annotated to the NCBI nr database (13 396, 35.92%), exhibiting highest homology with sequences from Lucilia cuprina. Further, a total of 9255 unigenes were annotated to three functional categories and 54 subclasses under the Gene Ontology (GO) database. Meanwhile, 6817 unigenes were assigned to 43 biochemical pathways under KEGG database, of which the signal transduction pathway was the most enriched. Moreover, 28 250 simple sequence repeats were found to be located in 16 538 unigenes, and A/T was the dominant repeat in 275 repeated motif types. The results of this study will lay a theoretical foundation for the development of biological control strategies for G. nasalis.

show abstract

“…The number of A. pegreffii and hybrids obtained in this study were scarce to perform a robust genetic population study. Thus, mtDNA cox2 gene for all the identified A. simplex was also sequenced since it is a common marker used for studies of population structure of the genus Anisakis [34,[37][38][39] and of numerous species of parasites [40][41][42][43], due to their high substitution rate and maternal inheritance.…”

Section: Genetic Identification Of Anisakis Species Of European Hakementioning

confidence: 99%

Population Genetic Structure of Anisakis simplex Infecting the European Hake from North East Atlantic Fishing Grounds

Ramilo

Rodríguez

Pascual

et al. 2023

Animals

View full text Add to dashboard Cite

The European hake, one of the most commercially valuable species in ICES fishing areas, is considered an important neglected source of zoonotic risk by nematode parasites belonging to the genus Anisakis. Merluccius merluccius is, by far, the most important host of Anisakis spp. at the European fishing grounds, in terms of demographic infection values, and carries the highest parasite burden. These high parasite population densities within an individual fish host offer a chance to explore new sources of variations for the genetic structure of Anisakis spp. populations. A total of 873 Anisakis spp. third-stage larvae, originally sampled from viscera and muscular sections of hake collected at ten fishing grounds, were primarily identified using ITS rDNA region as molecular marker. After that, we used mtDNA cox2 gene to reveal the high haplotype diversity and the lack of genetic structure for A. simplex. Dominant haplotypes were shared among the different fishing areas and fish sections analyzed. Results indicate a clear connection of A. simplex from European hake along the Northern North Sea to the Portuguese coast, constituting a single genetic population but revealing a certain level of genetic sub-structuring on the Northwest coast of Scotland. This study also provides useful information to advance the understanding of parasite speciation to different fish host tissues or microenvironments.

show abstract

Genetic diversity of common Gasterophilus spp. from distinct habitats in China

Cited by 9 publications

References 27 publications

The impact of temperature on the life cycle of Gasterophilus pecorum in northwest China

The impact of temperature on the life cycle of Gasterophilus pecorum in northwest China

De novo assembly and SSR loci analysis in Gasterophilus nasalis (Diptera: Oestridae)

Population Genetic Structure of Anisakis simplex Infecting the European Hake from North East Atlantic Fishing Grounds

Contact Info

Product

Resources

About