Genetic diversity of cucumber (Cucumis sativus) accessions differing in quantitative traits and microsatellite markers

Kumar, Rahul; Munshi, A. D.; Behera, Tusar Kanti; Jat, Gograj Singh; Choudhary, Harshawardhan; Singh, Mamta; Talukdar, Akshay

doi:10.56093/ijas.v90i11.108583

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…However, the PIC value ranged from 0.06 to 0.81 (mean 0.38); slightly higher than the PIC value (3.69) reported by [31] and 0.17 reported by [37], and lower than the PIC value (4.29) reported by [19]. The presence of a slightly high level of PIC in 96 bitter gourd genotypes of the present study might be due to high cross-pollination behaviour and similar findings were reported in other cross-pollinated crops such as cucumber [38,39].…”

Section: Discussionsupporting

confidence: 64%

Genetic Diversity and Population Structure Analyses in Bitter Gourd (Momordica charantia L.) Based on Agro-Morphological and Microsatellite Markers

Mallikarjuna,

Tomar,

Mangal

et al. 2023

Plants

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Bitter gourd (Momordica charantia L.) is an important vine crop of the Cucurbitaceae family and is well known for its high nutritional and medicinal values. However, the genetic variation remains largely unknown. Herein, 96 diverse bitter gourd genotypes were undertaken for diversity analysis using 10 quantitative traits, and 82 simple sequence repeat (SSR) markers. Out of 82 SSRs, 33 were polymorphic and the mean polymorphism information content (PIC) value was 0.38. Marker, JY-003 revealed a maximum (0.81) PIC value and, the number of alleles per locus ranged from 2 to 7 (average 3.46). The value of gene diversity showed the presence of a significant level of polymorphism among these genotypes. The unweighted pair group method (UPGMA) cluster analysis grouped the genotypes into two major clusters of which Cluster I comprised mostly small and medium-fruited genotypes of both M. charantia var. charantia and M. charantia var. muricata, whereas Cluster II included mostly long and extra-long fruited genotypes. Furthermore, these genotypes were divided into six distinct groups based on population structure analysis. The diversity analysis based on 10 quantitative traits revealed that earliness and high-yielding ability were exhibited by the predominantly gynoecious line DBGS-21-06 followed by DBGS-48-00. The principal component analysis (PCA) revealed that the first two components exhibited more than 50% of the total genetic variation. The present study deciphered a higher magnitude of agro-morphological and genetic diversity in 96 bitter gourd genotypes. Therefore, trait-specific genotypes identified in this study could be utilized in breeding programmes directed towards the development of improved cultivars and hybrids of bitter gourd.

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Section: Discussionsupporting

confidence: 64%

Genetic Diversity and Population Structure Analyses in Bitter Gourd (Momordica charantia L.) Based on Agro-Morphological and Microsatellite Markers

Mallikarjuna,

Tomar,

Mangal

et al. 2023

Plants

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“…reported similar results for fruit diameter (4.16 to 6.82 cm). Signi cant variations were observed for individual fruit weight among genotypes and it ranged from 130.33 g − 334 g Kumar et al (2020). have reported similar results in cucumber accessions (124.63 g − 417.55 g).…”

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confidence: 52%

Fruit flesh thickness analysis in local cucumber (Cucumis sativus L.) genotypes through morphological and marker-based assay

Murthy,

Subbareddy,

et al. 2024

Preprint

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Cucumber (Cucumis sativus L.) is one of the commercially important and widely cultivated cucurbitaceous vegetable grown throughout the world. The immature fruits are consumed as salad or pickled. The present study involved 22 local cucumber genotypes collected from different places of Karnataka, India. Cucumber flesh thickness is an important trait for cucumber fruit quality and a central determinant of yield. Among the evaluated genotypes, the fruit flesh thickness (1.92 cm), fruit diameter (6.87 cm) and yield per plant (3.05 kg) were higher in genotype (CSSL) collected from Shiralakoppa (Shivamogga) region. The higher fruit length (31.48 cm) and lower fruit flesh thickness (0.84 cm) was recorded in genotype (VLD) collected from Dharwad. The analysis of variance revealed significant differences for all the traits. Higher GCV, PCV and high heritability coupled with high GAM were recorded for fruit weight, fruit diameter, fruit flesh thickness, seed cavity width and yield per plant. The fruit flesh thickness in 22 local cucumber genotypes was analysed using candidate gene (Csa2M058670.1) markers. Among 13 SSR primers, SSR23420 and SSR10849 have shown higher PIC (0.37) and higher gene diversity (0.50). The cucumber genotypes such as CSSL, CSWL, CSHLC-1 and CSHL were found to be promising for fruit quality traits such as fruit length, diameter, flesh thickness and yield.

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Association mapping, trait variation, interaction and population structure analysis in cucumber (Cucumis sativus L.)

Kumar

Munshi

Behera

et al. 2022

Genet Resour Crop Evol

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In several regions of the world, low productivity in this crop is attributed to several factors including poor understanding of the genomic complexity of important traits associated with fruit quality and yield. Therefore, genome wide association analysis was performed for important traits using simple sequence repeats (SSR) markers. Signi cant variation was recorded for all the studied traits in 78 cucumber genotypes under two environments (open eld and net house) which indicated that the constituted association panel was suitable for association mapping. Genotyping was done using 60 highly polymorphic SSRs. By performing genome scanning out of 60 SSR markers, using mixed linear model (MLM) approach 4 and 6 markers explained an average of 23.93% and 17.37 % of the trait variation under net house and open eld condition, respectively. Based on MLM approach two markers on 3 rd chromosome (UW084942) and 4 th chromosome (UW062953) found to be associated with the average fruit weight (g) under both net house and open eld condition. Population structure analysis revealed four distinct sub-populations that corroborated with the geographical origin as well as fruit quality and quantitative traits. The four sub-populations (A-D) had xation index percentage (Fst) equal to 24.35 29.48, 37.17 and 8.97 respectively, supporting the existence of moderate population structures. Therefore, the extensive phenotypic and genotypic characterization, population structure, and markers associated with critical traits provided in this study will facilitate marker assisted improvement programs in cucumber.

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Genetic diversity of cucumber (Cucumis sativus) accessions differing in quantitative traits and microsatellite markers

Cited by 4 publications

References 18 publications

Genetic Diversity and Population Structure Analyses in Bitter Gourd (Momordica charantia L.) Based on Agro-Morphological and Microsatellite Markers

Genetic Diversity and Population Structure Analyses in Bitter Gourd (Momordica charantia L.) Based on Agro-Morphological and Microsatellite Markers

Fruit flesh thickness analysis in local cucumber (Cucumis sativus L.) genotypes through morphological and marker-based assay

Association mapping, trait variation, interaction and population structure analysis in cucumber (Cucumis sativus L.)

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