Genetic diversity of dengue virus serotypes 1 and 2 in the State of Paraná, Brazil, based on a fragment of the capsid/premembrane junction region

Bona, Ana Caroline Dalla; Twerdochlib, Adriana Lacerda; Navarro-Silva, Mário Antônio

doi:10.1590/s0037-86822012000300003

Cited by 8 publications

(4 citation statements)

References 20 publications

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“…DENV-2 serotypes from Manaus were grouped with the Asian/American genotype that is more virulent than the indigenous American genotype and is also the predominant genotype in Brazil [16], [31]. DENV-3 belonged to genotype III introduced from the Caribbean Islands and present in Brazil since the last 15 years [32], [33].…”

Section: Discussionmentioning

confidence: 99%

Clinical and Virological Descriptive Study in the 2011 Outbreak of Dengue in the Amazonas, Brazil

et al. 2014

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BackgroundDengue is a vector-borne disease in the tropical and subtropical region of the world and is transmitted by the mosquito Aedes aegypti. In the state of Amazonas, Brazil during the 2011 outbreak of dengue all the four Dengue virus (DENV) serotypes circulating simultaneously were observed. The aim of the study was to describe the clinical epidemiology of dengue in Manaus, the capital city of the state of the Amazonas, where all the four DENV serotypes were co-circulating simultaneously.MethodologyPatients with acute febrile illness during the 2011 outbreak of dengue, enrolled at the Fundação de Medicina Tropical Dr. Heitor Viera Dourado (FMT-HVD), a referral centre for tropical and infectious diseases in Manaus, were invited to participate in a clinical and virological descriptive study. Sera from 677 patients were analyzed by RT-nested-PCRs for flaviviruses (DENV 1–4, Saint Louis encephalitis virus-SLEV, Bussuquara virus-BSQV and Ilheus virus-ILHV), alphavirus (Mayaro virus-MAYV) and orthobunyavirus (Oropouche virus-OROV).Principal FindingsOnly dengue viruses were detected in 260 patients (38.4%). Thirteen patients were co-infected with more than one DENV serotype and six (46.1%) of them had a more severe clinical presentation of the disease. Nucleotide sequencing showed that DENV-1 belonged to genotype V, DENV-2 to the Asian/American genotype, DENV-3 to genotype III and DENV-4 to genotype II.ConclusionsCo-infection with more than one DENV serotype was observed. This finding should be warning signs to health authorities in situations of the large dispersal of serotypes that are occurring in the world.

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Section: Discussionmentioning

confidence: 99%

Clinical and Virological Descriptive Study in the 2011 Outbreak of Dengue in the Amazonas, Brazil

et al. 2014

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“…Infection of A. aegypti by different viruses during outbreaks and epidemics leads to high levels of viral replication. This phenomenon could lead to genetic diversity in the dengue virus, which has serious consequences such as increasing virulence and pathogenic properties (Bona et al 2012). DHF and DSS cases are severe in dengue virus infection, most of which are caused by secondary infection by heterologous viral serotypes or primary infection with highly virulent genotypes.…”

Section: Phylogenetics and Genetic Diversitymentioning

confidence: 99%

Molecular detection and analysis of dengue virus genetic diversity in North Sulawesi, Indonesia during 2022

et al. 2023

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Abstract. Datu AM, Natzir R, Yustisia I, Wahid I, Soraya GV, Kadir S. 2023. Molecular detection and analysis of dengue virus genetic diversity in North Sulawesi, Indonesia during 2022. Biodiversitas 24: 3407-3413. Dengue is a highly infectious disease caused by the dengue virus, consisting of four serotypes (DENV1-DENV4). The existence of diverse serotypes and genotypes in an area significantly impacts the degree of virulence in humans, clinical manifestation, and the epidemiology of the disease. Studying virus diversity in an area is pivotal, especially in dengue-endemic areas such as North Sulawesi. This study was an explorative research analyzing dengue virus serotypes and sequencing on the C-PrM protein region to determine the genetic diversity. This study was conducted on children and adults in 3 health centers in North Sulawesi, Indonesia, in 2022. Of 137 sera samples from febrile patients with dengue-like symptoms, ten were confirmed positive for dengue virus. Out of the ten positive samples, DENV1 was the dominant serotype, which was 70%. This was followed by 20% DENV2 and 10% DENV4. The genotype of each serotype, DENV1, DENV2, and DENV4, was respectively classified into genotype I, cosmopolitan genotype, and genotype I as a new genotype of DENV4 reported in North Sulawesi. This study provides the latest data on the serotypes and genotypes of dengue viruses in North Sulawesi. Molecular surveillance of the dengue virus in North Sulawesi must be continuously conducted due to the circulation of multiple serotypes and genotypes in this province.

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“…In addition, such protocols are employed to detect dispersal and virulence patterns related to the outbreaks, besides the geographic origin of a given lineage. The DENV capsid and pre-membrane junction encoding gene (CprM) is used to characterize the genetic variation of the virus in endemic areas of Brazil 21 .…”

Section: Introductionmentioning

confidence: 99%

Epidemiological and genetic aspects of the largest dengue outbreak recorded in 2015 in Southeastern Brazil

Cabral

Oliveira

Molina

et al. 2021

Rev. Inst. Med. trop. S. Paulo

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Dengue virus, the etiological agent of dengue fever (DF) occurs in four genetically distinct serotypes (DENV1-4), being transmitted by female Aedes mosquitoes. DF incidence is increasing in Brazil, following vector dispersal, proliferation and DENV serotypes introduction, co-circulation and substitution. Medium- and small-sized cities in Sao Paulo State, such as Marilia (Midwest region), have been affected by huge epidemics. To understand the evolution of DENV epidemics in medium-sized cities, in this study a historical data on DENV incidence (2000-2015) in Marilia, was evaluated. Previous studies disclosed regional and specific DF outcomes associated with 2007 outbreak in that city, when co-circulating DENV1 and DENV3 presented different hematological profiles. In this study, characteristics of 2007 DENV epidemics were compared to the epidemiological, hematological and demographic outlines of the major outbreak of DENV1 in Marilia in 2015. DENV1 genetic diversity was assessed through capsid and pre-membrane junction encoding gene (CprM) sequencing. The results revealed circulation of DENV1 serotype from 2007 to 2015, with epidemics occurring every three-years until 2013 and then, increasing yearly. There were significant differences in hematological profiles of DENV1 patients between 2015 and 2007. CprM showed DENV1 genetic variability in 2015, contrasting with the unique sequence pattern in 2007. These results reinforce the regional and temporal characteristics of DENV epidemics that need local public health research to improve care for people and to limit the spread of new serotypes/genotypes to uninfected areas.

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Genetic diversity of dengue virus serotypes 1 and 2 in the State of Paraná, Brazil, based on a fragment of the capsid/premembrane junction region

Cited by 8 publications

References 20 publications

Clinical and Virological Descriptive Study in the 2011 Outbreak of Dengue in the Amazonas, Brazil

Clinical and Virological Descriptive Study in the 2011 Outbreak of Dengue in the Amazonas, Brazil

Molecular detection and analysis of dengue virus genetic diversity in North Sulawesi, Indonesia during 2022

Epidemiological and genetic aspects of the largest dengue outbreak recorded in 2015 in Southeastern Brazil

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