Genetic Diversity of Double-Stranded RNA from<i>Rhizoctonia solani</i>

Bharathan, N.

doi:10.1094/phyto-80-631

Cited by 45 publications

(31 citation statements)

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“…However, the phenotypic effects were not always consistent because different dsRNAs correlated differently with virulence (Bharathan and Tavantzis, 1990). Jian et al (1997) subcultured sectors from a dsRNA-containing isolate in an effort to obtain isolates with specific combinations of segments.…”

Section: Virus-mediated Increases Inmentioning

confidence: 99%

The Ecology and Evolution of Fungal Viruses

Milgroom

Hillman

2011

Studies in Viral Ecology

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Section: Virus-mediated Increases Inmentioning

confidence: 99%

The Ecology and Evolution of Fungal Viruses

Milgroom

Hillman

2011

Studies in Viral Ecology

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“…have been discovered since double-stranded RNA (dsRNA) was reported to be associated with Rhizoctonia decline in 1978 [2][3][4][5]. Mycoviruses have been found ubiquitously in major groups of filamentous fungi [6], and dsRNA viruses were commonly detected in natural populations of multinucleate R. solani isolates AG-1 to -13 [4,[7][8][9] and binucleate Rhizoctonia isolates AG-D [10], AG-A, AG-F, AG-R, and AG-U [11]. It has been suggested that some dsRNAs might affect the degree of pathogenicity (virulence) in R. solani [9].…”

Section: Introductionmentioning

confidence: 99%

“…Mycoviruses have been found ubiquitously in major groups of filamentous fungi [6], and dsRNA viruses were commonly detected in natural populations of multinucleate R. solani isolates AG-1 to -13 [4,[7][8][9] and binucleate Rhizoctonia isolates AG-D [10], AG-A, AG-F, AG-R, and AG-U [11]. It has been suggested that some dsRNAs might affect the degree of pathogenicity (virulence) in R. solani [9]. A single isolate of R. solani has been shown to harbor two dsRNAs that have opposite effects on the pathogenicity to its plant host, and the degree of pathogenicity of the respective isolate was determined by the relative concentrations of the two dsRNA species [12,13].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of a novel mycovirus from Rhizoctonia fumigata AG-Ba isolate C-314 Baishi

Zhang

et al. 2015

Arch Virol

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The complete genome sequence of a novel dsRNA virus isolated from Rhizoctonia fumigata AG-Ba isolate C-314 Baishi (designated as Rhizoctonia fumigata virus 1, RfV1) was determined. The RfV1 genome was 9,907 bp in length and contained two open reading frames (ORFs). ORF1 potentially coded for a 198.10-kDa protein (P1). P1 shared low but significant amino acid sequence similarity to the putative protein encoded by Lentinula edodes mycovirus (LeV) ORF1. P1 contained a NUDIX domain, which was also present in the putative proteins encoded by the ORF1s of LeV and Phlebiopsis gigantea large virus 1 (PgLV-1). ORF2 potentially coded for a 146.72-kDa protein (P2) that contained the conserved motifs of the RNA-dependent RNA polymerase (RdRp). ORF1 and ORF2 were overlapping, and it was predicted that ORF2 could be translated as a fusion with ORF1 via a ribosomal -1 frameshifting mechanism. Phylogenetic analysis indicated that RfV1 clustered with PgLV-1, LeV and Rosellinia necatrix megabirnavirus 1 (RnMBV1) in a separate clade independent of other virus genera. We propose that RfV1, along with PgLV-1 and LeV, should be grouped into a new viral genus related to the family Megabirnaviridae. This is the first report of the full-length genome sequence of a novel mycovirus isolated from R. fumigata.

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Section: Introductionmentioning

confidence: 99%

“…We have also shown that the conflicting reports on the role of dsRNAs in R. solani could be attributed, at least in part, to the high degree of genetic diversity among dsRNA elements occurring in natural populations of the pathogen (10, 11). More importantly, we have presented several lines of indirect evidence suggesting that specific dsRNA elements might be involved in up-or down-regulation of virulence in R. solani (10)(11)(12)(13)(14).Recently, we described a genetic model wherein new dsRNAs appear or existing dsRNAs disappear from a given genotype (Rhs 1AP) with concomitant changes in virulence (15). Rhs 1AP is a virulent culture, member of anastomosis group 3, which is the major cause of the rhizoctonia disease syndrome of potato in North America (16,17).…”

mentioning

confidence: 99%

A double-stranded RNA element from a hypovirulent strain of Rhizoctonia solani occurs in DNA form and is genetically related to the pentafunctional AROM protein of the shikimate pathway

Lakshman

Jian²,

Tavantzis³

1998

Proc. Natl. Acad. Sci. U.S.A.

116

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M2 is a double-stranded RNA (dsRNA) element occurring in the hypovirulent isolate Rhs 1A1 of the plant pathogenic basidiomycete Rhizoctonia solani. Rhs 1A1 originated as a sector of the virulent field isolate Rhs 1AP, which contains no detectable amount of the M2 dsRNA. The complete sequence (3,570 bp) of the M2 dsRNA has been determined. A 6.9-kbp segment of total DNA from either Rhs 1A1 or Rhs 1AP hybridizes with an M2-specific cDNA probe. The sequences of M2 dsRNA and of PCR products generated from Rhs 1A1 total DNA were found to be identical. Thus this report describes a fungal host containing full-length DNA copies of a dsRNA element. A major portion of the M2 dsRNA is located in the cytoplasm, whereas a smaller amount is found in mitochondria. Based on either the universal or the mitochondrial genetic code of filamentous fungi, one strand of M2 encodes a putative protein of 754 amino acids. The resulting polypeptide has all four motifs of a dsRNA viral RNAdependent RNA polymerase (RDRP) and is phylogenetically related to the RDRP of a mitochondrial dsRNA associated with hypovirulence in strain NB631 of Cryphonectria parasitica, incitant of chestnut blight. This polypeptide also has significant sequence similarity with two domains of a pentafunctional polypeptide, which catalyzes the five central steps of the shikimate pathway in yeast and filamentous fungi.In the last three decades, fungal double-stranded RNA (dsRNA) elements have been the subject of considerable research because of their potential adverse effects on plant pathogenic fungi, and the prospects of utilizing them in biocontrol schemes against the host fungus (1-3). dsRNAs have been associated with cytoplasmic hypovirulence (4) or virulence (5) in Rhizoctonia solani. We have shown that dsRNAs are ubiquitous in natural R. solani populations that include isolates covering a wide range of virulence, including hypovirulence (6). Subsequent surveys conducted in Japan (7), Florida (8), and Louisiana (9) confirmed our findings. We have also shown that the conflicting reports on the role of dsRNAs in R. solani could be attributed, at least in part, to the high degree of genetic diversity among dsRNA elements occurring in natural populations of the pathogen (10, 11). More importantly, we have presented several lines of indirect evidence suggesting that specific dsRNA elements might be involved in up-or down-regulation of virulence in R. solani (10)(11)(12)(13)(14).Recently, we described a genetic model wherein new dsRNAs appear or existing dsRNAs disappear from a given genotype (Rhs 1AP) with concomitant changes in virulence (15). Rhs 1AP is a virulent culture, member of anastomosis group 3, which is the major cause of the rhizoctonia disease syndrome of potato in North America (16,17). Rhs 1A1 originated as a sector of Rhs 1AP and contains, in addition to the two dsRNAs of Rhs 1AP (L2 and M1), three genetically distinct dsRNAs (L1, M2, and S1). Molecular sizes of L1, L2, M1, M2, and S1 dsRNAs were estimated to be 25, 23, 6.4, 3.6, and 1.2 kbp,...

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Genetic Diversity of Double-Stranded RNA fromRhizoctonia solani

Cited by 45 publications

References 0 publications

The Ecology and Evolution of Fungal Viruses

The Ecology and Evolution of Fungal Viruses

Molecular characterization of a novel mycovirus from Rhizoctonia fumigata AG-Ba isolate C-314 Baishi

A double-stranded RNA element from a hypovirulent strain of Rhizoctonia solani occurs in DNA form and is genetically related to the pentafunctional AROM protein of the shikimate pathway

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