Genetic Diversity of Ethiopian Tef [(Eragrostis tef (Zucc.) Trotter] Released and Selected Farmers’ Varieties along with Two Wild Relatives as Revealed by Microsatellite Markers

Fikre, Tsion; Tesfaye, Kassahun; Assefa, Kebebew

doi:10.1007/s12892-018-0066-0

Cited by 12 publications

(7 citation statements)

References 23 publications

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“…The PCoA analysis in the two-dimensional plot displayed in ( Figure 1 ) showed that accessions from different collection sites often clustered together, showing possible gene flow. A similar finding was also reported by Fikre et al [ 30 ]. There was no separate group formed by a single population possibly because of different factors that favor for intermixing of the germplasm including gene flow and hybridization.…”

Section: Resultssupporting

confidence: 92%

“…Ayele [23] by using AFLP markers and Bai et al [24] by using RAPD markers reported genetic similarity coefficients of 85-90% and 84-96%, which indicated a high level of genetic similarity of the tested genotypes. Previous research [25][26][27][28][29][30][31] has shown the usefulness of SSR markers in genetic diversity analysis and in establishing a genetic relationship among tef germplasm. SSR markers are particularly useful since they are codominant, high reproducibility, and polymorphic, have low operational cost and locus-specificity, and revealing of high allelic diversity.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity of Tef [Eragrostis tef (Zucc.)Trotter] as Revealed by Microsatellite Markers

Tadesse

Kebede

Girma

2021

International Journal of Genomics

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Genetic variability is the fundamental prerequisite of any crop-breeding program to develop superior cultivars. There are about 350 Eragrostis1 species, of which, tef is the only species cultivated for human consumption. Currently, the Ethiopian Biodiversity Institute (EBI) collected over five thousand tef accessions from different geographical regions, diverse in terms of climate and elevation, which are uncharacterized yet. The objective of this study was to evaluate the genetic diversity among 64 tef accessions using 10 selected polymorphic simple sequence repeats (SSRs) markers. A total of 314 alleles were detected with an average of 14.5 alleles per locus and amplicon size ranged from 90 bp–320 bp. The mean value of polymorphic information content (PIC) was 0.87, appearing polymorphic for all loci. The lowest Fst value (0.05) was recorded among the studied tef populations. The mean value of major allele frequency and the number of effective alleles were 0.33 and 3.32, respectively. The mean value of gene flow (Nm) and Shannon’s information index (I) was 4.74 and 1.65, respectively. The observed (Ho) and expected (He) heterozygosities varied from 0.34 to 0.56 and from 0.58 to 0.76, respectively. The cluster analysis has grouped the 64 tef accessions into three distinct clusters based on their similarity. The PCoA analysis showed that clustering is basing on the geographical origin of accessions. Analysis of molecular variance revealed 56%, 39% and 5% of the total variation due to variation within populations, among individuals and among populations, respectively. Structure bar-plot also inferred three gene pools, but with high level of admixtures. Thus, the present study shows that the identified tef accessions could be of great interest for the initiation of a planned breeding and conservation programs.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Tef [Eragrostis tef (Zucc.)Trotter] as Revealed by Microsatellite Markers

Tadesse

Kebede

Girma

2021

International Journal of Genomics

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“…In the last few decades, research conducted results revealed that N amount, methods and time of application determines not only the fate of applied N, but also optimizes the crop output under rainfed Tef production (Dereje et al, 2018;Gedamu et al, 2023;Habtegebrial et al, 2007;Negassa & Abera, 2013). The findings from these studies showed that the increasing rates of N application influences the yield parameters while the shortage of N supply decreases yield (Fikre et al, 2018;.…”

Section: Introductionmentioning

confidence: 96%

Evaluation of grain protein content in Eragrostis tef for different N fertilizer application under irrigated condition

Gebremeskel

2023

JINAGRI

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The reason for the low productivity of the northern part of Ethiopia. A field experiment was conducted to examine the impact of planting method and nitrogen fertilizer rates on the yield and protein content of irrigated Tef. The experiment consisted of four planting me planting, and transplanting) and six N fertilizer rate (0, 23, 46, 69,92 and 115 kg ha Design (RCBD) with four replications. Seven response variables, heading date, matu above ground biomass yield, grain yield, and grain protein content of Tef have been collected and analyzed. Analysis of variance showed that all parameters, with the exception of grain protein content, significantly affected (P<0.05) by the interacting effects of planting methods and nitrogen levels. The highest values of all parameters were obtained from transplanting and N rate of 92 kg ha plants receiving this treatment were extr Innovative Agriculture

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“…Ethiopian institutes hold the largest tef collection with about more than 5000 diverse tef accessions collected from different agro-ecological zones. This could provide the resources for the derivation of elite tef genotypes (Girma et al 2014 ; Fikre et al 2018 ). The key environmental constraints that affect tef productivity are lodging, drought and salinity and high diversity between tef cultivars such as seed color, plant height, leaf traits and the relatively long life cycle which makes the screening of stress tolerance traits within tef cultivars difficult (Woldeyohannes et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Metabolomics targets tissue-specific responses in alleviating the negative effects of salinity in tef (Eragrostis tef) during germination

Haddadi,

Fang,

Girija

et al. 2023

Planta

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Main conclusion Salinity induced metabolite responses resulted in differential accumulation of flavonoids and antioxidant metabolites in shoots and roots suggesting improved antioxidant capacity in providing salt-adaptive phenotype of tef seedling. Abstract Tef [(Eragrostis tef) (Zucc.) Trotter] is an important ‘cash crop’ of Ethiopia grown mainly for human food, and development of elite tef cultivars with better performance is vital to Ethiopian farmers and breeders. Soil salinity is one of the key constraints that affects tef yield in the Ethiopian lowlands and Rift valley where cultivation of tef is limited. Being a minor crop, the responses of tef towards salinity is unknown. Salinity involves physiological and metabolite reprogramming that can have major impact on germination and seedling establishment. Here we evaluate the in vitro effect of NaCl on tef germination and associate this with metabolomic approaches to suggest salt tolerance mechanisms. In this study, 19 tef varieties were screened for NaCl tolerance and were investigated using untargeted metabolomics. Screened tef varieties showed differential germination rates with NaCl treatment varying from < 20 to 100%. Viable seedlings exposed to NaCl exhibited purple-red pigment accumulation in the roots except for Beten and Tullu nasy varieties. Metabolite comparisons between shoots and roots showed significant differences and, in particular, roots of salt tolerant tef varieties accumulated flavonoid derivatives as well as sugars and cell wall associated metabolites. These metabolic changes were correlated with patterns of antioxidant capacities and total flavonoid content in shoots and roots and suggested a mitigating response by tef to salinity. Our study highlights the role of flavonoid accumulation following salt stress on tef seedlings and further these findings could be used as targets for selective tef breeding.

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Genetic Diversity of Ethiopian Tef [(Eragrostis tef (Zucc.) Trotter] Released and Selected Farmers’ Varieties along with Two Wild Relatives as Revealed by Microsatellite Markers

Cited by 12 publications

References 23 publications

Genetic Diversity of Tef [Eragrostis tef (Zucc.)Trotter] as Revealed by Microsatellite Markers

Genetic Diversity of Tef [Eragrostis tef (Zucc.)Trotter] as Revealed by Microsatellite Markers

Evaluation of grain protein content in Eragrostis tef for different N fertilizer application under irrigated condition

Metabolomics targets tissue-specific responses in alleviating the negative effects of salinity in tef (Eragrostis tef) during germination

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