Genetic diversity of exotic and local eggplants (Solanum spp.) cultivated in Côte d’Ivoire based on ISSR markers

Abstract

Abstract. Konan NO, Akaffou MA, Kouadio L, Akaffou DS, Mergeai G. 2020. Genetic diversity of exotic and local eggplants (Solanum spp.) cultivated in Côte d’Ivoire based on ISSR markers. Biodiversitas 21: 3651-3658. Eggplants are important vegetables in Côte d'Ivoire. Despite an increase in the cultivation of exotic genotypes and their progressive dominance over local cultivars, which could represent a threat of genetic erosion, there are not enough molecular data available on the genetic diversity of the eggplants currently cultivated in this country. This study is a contribution to the knowledge of the genetic diversity of the current eggplant cultivars grown in Côte d’Ivoire. It aimed to assess the genetic variation of nine exotic and local eggplants commonly cultivated in Côte d'Ivoire, using five inter-simple sequence repeat (ISSR) primers. The results revealed for the exotic cultivars 42 ISSR loci of which 31 were polymorphic (73.81 %), and for local cultivars 51 loci of which 49 (96.08%) were polymorphic. The Nei’s genetic diversity and Shannon’s information index of local cultivars (He = 0.3333 and I = 0.4863) were higher than those of exotic cultivars (He = 0.2000 and I = 0.2971). The Gst value and the AMOVA analysis found more than 70% of the total genetic diversity within the groups. The Jaccard’s dissimilarity coefficients between the cultivars, ranged from 0.324 to 0.966 with an average of 0.741. The clustering of genotypes with the neighbor-joining unrooted tree and PCoA analysis differentiated three main clusters with exotic cultivars grouped in cluster I and local cultivars in cluster II and III. In view of these results, the local eggplants had higher genetic diversity and constitute an interesting germplasm which needs to be conserved for potential breeding programs.

Exploring the genetic makeup and population structure among Capsicum accessions for crop improvement and breeding curriculum insights

“…In the present study, an average GC-content of the 7 selected ISSR markers was 54.28% which is in consistent with previous reports [31,71]. A total of 80 bands were generated from 7 ISSR primers selected in the present study, and the average frequency of banding pattern was 11.43 bands per primer, while in another study in Capsicum, 2 ISSR primers amplified a total 38 bands with an average of 19 bands per primer [61].…”

“…The plasticity in the population structures was evaluated by AMOVA and Jaccard's similarity coefficient, and the result of AMOVA represented a significant genetic variation within the population of Capsicum accessions with 89% of total variability and 11% among the population of chillies. Such analysis have been reported for Glycyrrhiza uralensis [96], Parkia timoriana [97], Trachyspermum ammi [98], Melocanna baccifera [69], and Solanum species [71]. The result of the ANOVA is in consistent with Gst and Nm values wherein increased genetic differentiation was observed within population and reduced gene flow among the population.…”

Improving Earliness-Related Traits of Egyptian Local Okra Cultivar (Abelmoschus esculentus L. Moench)

“…So, selection of lines based on individual attributes may not be beneficial as the one based on a number of important traits taken together. These has been agreement with many researchers such as Binalfew and Alemu (2016), Amiteye et al (2019) and Konan et al (2020) who used cluster analysis to classify some okra genotypes based on their morphological characteristics.…”