Genetic diversity of five Chinese goat breeds assessed by microsatellite markers

Li, JianMei; Chen, H.; Lan, Xianyong; Kong, Xiaochao; Liu, Min

doi:10.17221/347-cjas

Cited by 23 publications

(19 citation statements)

References 11 publications

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“…In the present study, about 81% of the loci showed positive deviation from Hardy-Weingberg equilibrium. The mean observed and expected heterozygosities obtained in this study is higher than that reported in other goat breeds [5,12,16]. The fairly high value of inbreeding estimate (F IS ) indicated that some of the loci in this breed were homozygous presumably resulting from the mating between relatives and consequent genetic drift.…”

Section: Resultscontrasting

confidence: 67%

Genetic architecture and bottleneck analyses of Salem Black goat breed based on microsatellite markers

Thiruvenkadan¹,

Jayakumar²,

Kathiravan³

et al. 2014

Vet World

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Aim: The present study was undertaken in Salem Black goat population for genetic analysis at molecular level to exploit the breed for planning sustainable improvement, conservation and utilization, which subsequently can improve the livelihood of its stakeholders. Materials and Methods:Genomic DNA was isolated from blood samples of 50 unrelated Salem Black goats with typical phenotypic features in several villages in the breeding tract and the genetic characterization and bottleneck analysis in Salem Black goat was done using 25 microsatellite markers as recommended by the Food and Agricultural Organization, Rome, Italy. The basic measures of genetic variation were computed using bioinformatic software. To evaluate the Salem Black goats for mutation drift equilibrium, three tests were performed under three different mutation models, viz., infinite allele model (IAM), stepwise mutation model (SMM) and two-phase model (TPM) and the observed gene diversity (He) and expected equilibrium gene diversity (Heq) were estimated under different models of microsatellite evolution. Results:The study revealed that the observed number of alleles ranged from 4 (ETH10, ILSTS008) to 17 (BM64444) with a total of 213 alleles and mean of 10.14±0.83 alleles across loci. The overall observed heterozygosity, expected heterozygosity, inbreeding estimate and polymorphism information content values were 0.631±0.041, 0.820±0.024, 0.233±0.044 and 0.786±0.023 respectively indicating high genetic diversity. The average observed gene diversities (He) pooled over different markers was 0.829±0.024 and the average expected gene diversities under IAM, TPM and SMM models were 0.769±0.026, 0.808±0.024 and 0.837±0.020 respectively. The number of loci found to exhibit gene diversity excess under IAM, TPM and SMM models were 18, 17 and 12 respectively. Conclusion:All the three statistical tests, viz., sign test, standardized differences test and Wilcoxon sign rank test, revealed significant deviation of Salem Black goats from mutation-drift equilibrium under IAM and TPM models, however, nonsignificant deviation under SMM model. The qualitative test of mode shift analysis supported the results under SMM indicating the absence of the genetic bottleneck in the recent past in Salem Black goats.

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Section: Resultscontrasting

confidence: 67%

Genetic architecture and bottleneck analyses of Salem Black goat breed based on microsatellite markers

Thiruvenkadan¹,

Jayakumar²,

Kathiravan³

et al. 2014

Vet World

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“…Comprehensive knowledge of genetic diversity is the first step for conservation of a given population (LI et al 2008). The results of the present work indicate a similarity of the subpopulations.…”

Section: Microsatellite Markerssupporting

confidence: 56%

Inter and Intra Subpopulation Genetic Variability of Roe Deer (Capreolus capreolus L.) Assessed by I and II Class Genetic Markers

Kamieniarz¹,

Wolc²,

Lisowski³

et al. 2011

folia biol (krakow)

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The material was collected in three regions of Poland and consisted of 105 randomly chosen individuals killed during hunts (49 males, 56 females), out of which 51 were from Wielkopolska, 22 from Podkarpacie and 32 from Warmia. From each animal a blood sample was taken from the chest, stored in a probe with K EDTA and frozen. The serum was used to establish the genotype for transferin and albumin whereas the samples with erythrocytes provided information on hemoglobin genotype. DNA was isolated from samples from each individual. Characteristics of eight (from among twelve studied) microsatellite loci and genetic distances were estimated by the use of standard computer package programs.Generally, monomorphism in blood proteins was registered. For the microsatellite loci the number of alleles ranged from 3 in the RT27-6-Fa locus (effectively two as the third allele was present only in two subpopulations with a very low frequency) to 10 in RT1-VI. Five loci showed heterozygosity of 0.5 or above which suggests their usefulness in parentage control.Considerable genetic distances (corresponding to geographical mileages) between the subpopulations were observed based on microsatellite markers.

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“…Moradii et al (2014) showed that the phylogenetic dendrogram of color types using ISSR markers was in agreement with their phenotypic similarities, in which saddle brown and sandy brown color types were grouped in one cluster and black types in another one. However, the current study is the first report using ISSR marker analysis to investigate the possibility of different clustering for the twining trait in several goat populations (Agha et al, 2008;Dixit et al, 2012;Javanrouh Aliabad et al, 2004;Li et al, 2008;Mirhoseini and Badbarin, 2013).…”

Section: Discussionmentioning

confidence: 96%

Genetic diversity and distance of Iranian goat breeds (Markhoz, Mahabadi and Lori) compared to the Beetal breed using inter-simple sequence repeat (ISSR) markers

et al. 2016

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Abstract. The aim of this study was to investigate the genetic diversity and structure in three Iranian native goat breeds (Markhoz, Mahabadi and Lori) and the Beetal imported breed using inter-simple sequence repeat (ISSR) markers and also to investigate ISSR markers' potential in order to genetically separate single (S) and twin-birth (T) subpopulations. Blood samples were collected from 210 animals for this purpose. In total, 16 primers were used, and finally 5 primers were selected based on the number of clear bands and the level of polymorphisms. The result of this study showed that 76 of 86 observed fragments were polymorphic. Genetic diversity for each breed ranged from 0.23 in the Beetal breed to 0.26 in the Markhoz breed; this represents a relatively similar genetic diversity in these breeds. An unweighted pair group method with arithmetic mean (UPGMA) dendrogram based on the Nei's standard genetic distance between the breeds studied showed that three Iranian goat breeds (Mahabadi, Lori and Markhoz) were clustered closer together, while the Beetal breed formed a separate cluster. In the constructed dendrogram of the subpopulations, the S and T subpopulations of each breed were clustered together. The constructed dendrogram of the Beetal breed and the S and T subpopulations of all breeds studied showed a separate cluster for the Beetal breed as an imported breed and another cluster for the S and T subpopulations as Iranian native breeds. The current study showed that the ISSR markers studied had no potential to genetically separate S and T subpopulations. On the other hand, these ISSR markers can be used for the clustering of distinct populations.

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Genetic diversity of five Chinese goat breeds assessed by microsatellite markers

Cited by 23 publications

References 11 publications

Genetic architecture and bottleneck analyses of Salem Black goat breed based on microsatellite markers

Genetic architecture and bottleneck analyses of Salem Black goat breed based on microsatellite markers

Inter and Intra Subpopulation Genetic Variability of Roe Deer (Capreolus capreolus L.) Assessed by I and II Class Genetic Markers

Genetic diversity and distance of Iranian goat breeds (Markhoz, Mahabadi and Lori) compared to the Beetal breed using inter-simple sequence repeat (ISSR) markers

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