Genetic diversity of freshwater pearl mussel (Hyriopsis cumingii) in populations from the five largest lakes in China revealed by inter-simple sequence repeat (ISSR)

Abstract

Triangle sail mussel (Hyriopsis cumingii) is the most important mussel species commercially exploited for freshwater pearl culture in China. Its genetic diversity was studied among populations from the five largest freshwater lakes of China, Poyang Lake (PY), Dongting Lake (DT), Taihu Lake (TH), Hongze Lake (HZ), and Chaohu Lake (CH), by the polymorphism of the inter-simple sequence repeats (ISSRs). Polymerase chain reaction (PCR) detections showed that a total of 62 loci were amplified from eight primers; of those, 31.88%, 31.15%, 31.03%, 27.27% and 26.92%, respectively, were polymorphic in each of the five populations across all genotypes tested. The average heterozygosities were 0.4747, 0.3274, 0.2366, 0.2099 and 0.2018 in each of these populations from PY, TH, DT, CH and HZ, respectively. Phylogenic analyses showed that PY and TH populations clustered with CH and HZ and formed a group, while the DT population on its own formed a separated branch. The smallest distance (0.0404) was scored between PY and TH populations, indicative of their closest relationship; the biggest distance (0.2438) was found between PY and DT populations, suggesting their greatest divergence. The present study provided genetic basis for managing mussel stocks from these lakes separately to best preserve the genetic diversity of H. cumingii. On the other hand, since the population in PY displayed the highest genetic diversity, it may be used preferably in future selective breeding to improve pearl yield and quality.

Molecular Cloning and Copy Number Variation of a Ferritin Subunit (Fth1) and Its Association with Growth in Freshwater Pearl Mussel Hyriopsis cumingii

“…To our knowledge, these are the first genotype data obtained for CNVs in freshwater bivalves. Our finding that there were more copy number variations in the wild population than in the cultured population is in agreement with other results obtained by simple sequence repeats and other markers in our laboratory [18], [48]. In the wild population, seven, eight, nine, and 10 copy number variations were the principal genotypes, which suggested the mussels with higher copy number in ferritin would be easier to survive in lake, and implied that ferritin dosage may convey a growth or resistance advantage.…”

Genetic Variability in Labeo rohita, Catla catla and their Hybrid (Labeo rohita ♂ ? Catla catla ♀) Populations Employing Randomly Amplified Polymorphic (RAPD)-Inter Simple Sequence Repeat (ISSR) Assays

“…As compared with RAPD markers, a larger number of loci/ fragments can be produced more rapidly with a limited number of primers to estimate the genetic diversity in a population. In a recent, study of Trachidermus fasciatus, it is reported that ISSRs are good DNA markers for diversity studies and furthermore the genetic differences in the hatchery populations are low as compared to wild populations (Li et al, 2009). These results were although for endangered fish species from China, but they verified the present finding about the genetic diversity of L. rohita, C. catla and their hybrids from hatchery stocks.…”

“…The size of the amplified loci ranged from 250bp to 1500bp which are in good agreement of previous ISSR studies. Li et al (2009) (Table 1). This low genetic variation may be due to inbreeding practices usually carried out at fish seed hatcheries.…”

Genetic diversity and geographic differentiation analysis of duckweed using inter-simple sequence repeat markers

“…Systematics of duckweed was classified according to the method of E. Landolt et al [3] and the number of populations and accessions was as following [23,[26][27][28][29][30]: 16 populations were from five species of genus Lemna and 11 populations from two species of genus Spirodela. Five Lemna species, Lemna minuta, Lemna minor, Lemna aequinoctialis, L. gibba, Lemna perpusilla, had 3, 5, 3, 3, 2 populations, respectively.…”