Genetic Diversity of Glu-1 in European Wheat Genetic Resources and Varieties

Hlozáková, Tímea Kuťka; Gregová, E.; Gálová, Zdenka

doi:10.15414/jmbfs.2015.4.special2.23-25

Cited by 7 publications

(4 citation statements)

References 7 publications

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“…Protein sizing with the chip-based protein analysis system was performed by running the protein sizing standard on each chip from a designated well. Following the analysis of this sizing standard, the software generated a standard curve of the measured migration time versus the known molecular weight of each standard protein which was used to determine the size of each of the proteins detected within the sample (Kuschel et al, 2002). Internal standards, the lower and upper marker, were included in each sample.…”

Section: Resultsmentioning

confidence: 99%

Determination of HMW - GS in wheat using SDS - PAGE and Lab-on-chip methods

Hlozáková

Gregová

Šliková

et al. 2019

Potr. S. J. F. Sci.

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SDS-PAGE is widely used to determine the amounts of the different gluten protein types. However, this method is time-consuming, especially at early stages of wheat breeding, when large number of samples needs to be analyzed. On the other hand, LoC (Lab-on-Chip) technique has the potential for a fast, reliable, and automatable analysis of proteins. Benefits and limitations of Lab-on-Chip method over SDS-PAGE method in gluten proteins evaluation were explored in order to determine in which way LoC method should be improved in order to make its results more compliant with the results of SDS-PAGE. Chip electrophoresis provides a very good reproducibility of HMW-GS patterns. Moreover this approach is much faster than the conventional SDS-PAGE methods requiring several hours for an analysis. Another advantage over traditional gel electrophoresis is lower sample and reagent volume requirements, as well as specialized protein standards for accurate reproducibility and quantification. In the present study, we identified novel complex allele located at the locus Glu-1B.

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Section: Resultsmentioning

confidence: 99%

Determination of HMW - GS in wheat using SDS - PAGE and Lab-on-chip methods

Hlozáková

Gregová

Šliková

et al. 2019

Potr. S. J. F. Sci.

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“…The possible reason for the higher polymorphism of storage proteins obtained for SDS -PAGE in the present study could be due to relatively high genetic diversity of HMW -GS. (Chňapek et al, 2013(Chňapek et al, , 2014Gregová et al, 2000;Kuťka Hlozáková et al, 2015;Oslovičová et al, 2010;). Some previous studies based on protein profile demonstrated that numbers of cultivars are indistinguishable (Marchylo and LaBerge, 1981;Shewry et al, 1978), because of that Ahmed et al (2010), Fouda et al (2011) and Shehata (2004) used also DNA marker such as RAPD to differentiate wheat genotypes.…”

Section: Rapd -Pcrmentioning

confidence: 99%

“…The aim of the present study was to provide molecular patterns for the identification and characterization of the phylogenetic relationships among twentyfour European cultivars of common wheat, included the genotype with probably novel highmolecularweight glutenin subunits (HMW -GS) identified by SDS -PAGE (Kuťka Hlozáková et al, 2015). This will be helpful in future for genomic mapping studies leading to obtain improved crop productivity with fresh genetic background.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of European cultivars of common wheat (Triticum aestivum L.) based on RAPD and protein markers

Hlozáková¹,

Gregová²,

Vivodík³

et al. 2016

JCEA

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The knowledge of genetic diversity of wheat genotypes based on DNA and protein polymorphism is very important for breeding programs (MAS). The objective of this study was to assess the genetic variability among twentyfour European cultivars of common wheat using SDS-PAGE and RAPD techniques. Protein electrophoreses showed 31 polymorphic bands with 63.2% polymorphism. Genetic similarity based on Jaccard´s coefficient ranged from 0.067 to 0.933. Also twelve different Glu-1 encoded allelic variants were identified among these 24 genotypes resulting from combination of 3 alleles of Glu-1A, 7 of Glu-1B and 2 of Glu-1D loci. One novel allelic variant 6.5+7.5 at the Glu-1B locus was identified. RAPD analysis showed that the number of polymorphic amplicons was 56 out of a total of 63 amplicons, thus revealing a level of 37.56% polymorphism. Maximum level of polymorphism (55.8%) was observed for the primer OPA-03 and minimum one for OPD-08 (13.8%). Genetic similarity based on Jaccard´s coefficient ranged from 0.396 to 0.805. As a result of this investigation, might be expected that the SDS-PAGE using protein markers and RAPD-PCR using DNA markers would be a useful tool for assessment of genetic diversity among wheat genotypes.

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“…It is well known that A × 2* and D × 5 + Dy10 is associated with good bread-making quality, especially dough strength, while D × 2 + Dy12 is associated with poor quality. The highest polymorphism of HMW-GS is regularly detected on 1B chromosome [ 4 , 5 , 6 , 7 ]. Nowadays, the most challenging task for wheat breeders is not only to increase grain yield [ 8 ] but also to improve the grain quality for end products.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Novel x-Type HMW Glutenin Subunit 1B × 6.5 in Wheat

Hlozáková

Gálová

Šliková

et al. 2021

Plants

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A novel high molecular weight glutenin subunit encoded by the Glu-1B locus was identified in the French genotype Bagou, which we named 1B × 6.5. This subunit differed in SDS-PAGE from well-known 1B × 6 and 1B × 7 subunits, which are also encoded at this locus. Subunit 1B × 6.5 has a theoretical molecular weight of 88,322.83 Da, which is more mobile than 1B × 6 subunit, and isoelectric point (pI) of about 8.7, which is lower than that for 1B × 6 subunit. The specific primers were designed to amplify and sequence 2476 bp of the Glu-1B locus from genotype Bagou. A high level of similarity was found between the sequence encoding 1B × 6.5 and other x-type encoding alleles of this locus.

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Genetic Diversity of Glu-1 in European Wheat Genetic Resources and Varieties

Abstract: Keywords: Storage proteins, HMW -GS, Triticum aestivum L.

Cited by 7 publications

References 7 publications

Determination of HMW - GS in wheat using SDS - PAGE and Lab-on-chip methods

Determination of HMW - GS in wheat using SDS - PAGE and Lab-on-chip methods

Genetic diversity of European cultivars of common wheat (Triticum aestivum L.) based on RAPD and protein markers

Molecular Characterization of Novel x-Type HMW Glutenin Subunit 1B × 6.5 in Wheat

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