Genetic diversity of Hajar1 and Hajar2 local Saudi chicken lines using mitochondrial DNA D-loop markers

Abstract

This study was conducted to assess genetic diversity of Hajar1 and Hajar2 local Saudi chicken lines using mitochondrial DNA (mtDNA) D-loop partial sequences. One hundred blood samples were obtained equally from Hajar1 and Hajar2 Saudi chicken lines as 50 samples from each line. The D-loop region was partially amplified from genomic DNA with a conserved primer set, and the fragments were sequenced. Eight published reference mtDNA sequence data from the GenBank were used for comparisons, and multiple alignments were performed. The most common haplotype was assigned as a basic sequence for comparing within each line. Entropy plot and conserved region analysis were performed. Genetic distances and neighbour-joining (NJ) phylogenetic trees were estimated. The results indicated haplotype variations within and between local Saudi chicken lines, which could explain the phenotypic variation reported earlier. A close genetic relationship was shown between the Saudi local chicken lines. Unique conserved regions and nucleotide substitutions were observed between the two lines. Both lines have a close relationship with the reference Asian local chicken population, especially local Chinese and Indian chicken breeds. The current results are considered the first report of mtDNA sequence diversity for Hajar1 and Hajar2 lines. Further detailed molecular genetic studies of both lines are indispensable to genetic conservation and development. ______________________________________________________________________________________

Genetic diversity of Saudi native chicken breeds segregating for naked neck and frizzle genes using microsatellite markers

“…It is an effective method to assess genetic diversity within and between chicken populations because they are highly polymorphic, show co-dominant inheritance, found to be abundant and evenly distributed throughout the genome [ 11 – 14 ]. Recently, few studies of genetic polymorphism were initiated on Saudi native chicken populations using mtDNA [ 15 , 16 ] or microsatellite markers [ 4 ]. Due to the limited mtDNA diversity, it is necessary to analyze autosomal markers in Saudi native breeds, in order to study diversity and the relationship between breeds.…”

Evaluation of Genetic Variability between Local White Chicken and Commercial Lines by RAPD-PCR and Sequencing of 18s rRNA Gene

“…Some of the DNA molecular markers discovered due to the fast development of present biotechnology include RAPD, SNP, AFLP, RFLP, and microsatellites [13], [14]. The Randomly Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) assays, first described by Welsh and Mc-Clelland (1990) and Williams et al (1990), have been used in poultry for determining genetic diversity and correlation in numerous species, as well as for determining potential markers to evaluate phylogenetic relationships of many various poultry species [15], [16], [17]. On the other hand, gene/genome sequencing, one of the most powerful current techniques, has been utilized to characterize biodiversity analyses in poultry and domesticated animals.…”