Genetic diversity of Histoplasma capsulatum isolated from infected bats randomly captured in Mexico, Brazil, and Argentina, using the polymorphism of (GA)n microsatellite and its flanking regions

Taylor, Mária Lucía; Hernández-García, L.; Estrada-Bárcenas, Daniel Alfonso; Salas‐Lizana, Rodolfo; Zancopé‐Oliveira, Rosely Maria; Cruz, Saúl García de la; Galvão-Dias, Maria A.; Curiel-Quesada, Everardo; Canteros, Cristina Elena; Bojórquez-Torres, Georgina; Bogard-Fuentes, Carlos A.; Tehozol, Erick Adrian Zamora

doi:10.1016/j.funbio.2011.12.004

Cited by 21 publications

(18 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Regarding the population structure of the H. capsulatum isolates studied, some of the Mexican isolates classified by Kasuga et al (11) were characterized as possibly recombinant, and others, such as environmental isolates from the Morelos State of Mexico, were characterized as a clonal population (11,(14)(15)(16).…”

Section: Resultsmentioning

confidence: 99%

“…Recent findings have involved the product of the Velvet A gene (VeA), which belongs to the proteins of the Velvet family, in mating structure formation (cleistothecial) and virulence of H. capsulatum (9). H. capsulatum isolates exhibit a wide distribution and an important genetic diversity, as has been documented in Latin America (10)(11)(12)(13)(14)(15)(16). However, the frequency and genetic diversity of the ϩ and Ϫ mating types are not well documented in most countries where H. capsulatum is found, and data reported in the United States are not necessarily representative of other geographical areas.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Frequency and Genetic Diversity of the MAT1 Locus of Histoplasma capsulatum Isolates in Mexico and Brazil

et al. 2013

Self Cite

View full text Add to dashboard Cite

The MAT1-1 and MAT1-2 idiomorphs associated with the MAT1 locus of Histoplasma capsulatum were identified by PCR. A total of 28 fungal isolates, 6 isolates from human clinical samples and 22 isolates from environmental (infected bat and contaminated soil) samples, were studied. Among the 14 isolates from Mexico, 71.4% (95% confidence interval [95% CI], 48.3% to 94.5%) were of the MAT1-2 genotype, whereas 100% of the isolates from Brazil were of the MAT1-1 genotype. Each MAT1 idiomorphic region was sequenced and aligned, using the sequences of the G-217B (؉ mating type) and G-186AR (؊ mating type) strains as references. BLASTn analyses of the MAT1-1 and MAT1-2 sequences studied correlated with their respective ؉ and ؊ mating type genotypes. Trees were generated by the maximum likelihood (ML) method to search for similarity among isolates of each MAT1 idiomorph. All MAT1-1 isolates originated from Brazilian bats formed a well-defined group; three isolates from Mexico, the G-217B strain, and a subgroup encompassing all soil-derived isolates and two clinical isolates from Brazil formed a second group; last, one isolate (EH-696P) from a migratory bat captured in Mexico formed a third group of the MAT1-1 genotype. The MAT1-2 idiomorph formed two groups, one of which included two H. capsulatum isolates from infected bats that were closely related to the G-186AR strain. The other group was formed by two human isolates and six isolates from infected bats. Concatenated ML trees, with internal transcribed spacer 1 (ITS1) -5.8S-ITS2 and MAT1-1 or MAT1-2 sequences, support the relatedness of MAT1-1 or MAT1-2 isolates. H. capsulatum mating types were associated with the geographical origin of the isolates, and all isolates from Brazil correlated with their environmental sources.

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Frequency and Genetic Diversity of the MAT1 Locus of Histoplasma capsulatum Isolates in Mexico and Brazil

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…capsulatum sensu stricto is lacking. In addition, regional genetic diversity of this pathogen has been assessed, but a meta-analysis of these data has not been completed [ 41 – 44 ].…”

Section: Introductionmentioning

confidence: 99%

Worldwide Phylogenetic Distributions and Population Dynamics of the Genus Histoplasma

et al. 2016

Self Cite

View full text Add to dashboard Cite

BackgroundHistoplasma capsulatum comprises a worldwide complex of saprobiotic fungi mainly found in nitrogen/phosphate (often bird guano) enriched soils. The microconidia of Histoplasma species may be inhaled by mammalian hosts, and is followed by a rapid conversion to yeast that can persist in host tissues causing histoplasmosis, a deep pulmonary/systemic mycosis. Histoplasma capsulatum sensu lato is a complex of at least eight clades geographically distributed as follows: Australia, Netherlands, Eurasia, North American classes 1 and 2 (NAm 1 and NAm 2), Latin American groups A and B (LAm A and LAm B) and Africa. With the exception of the Eurasian cluster, those clades are considered phylogenetic species.Methodology/Principal FindingsIncreased Histoplasma sampling (n = 234) resulted in the revision of the phylogenetic distribution and population structure using 1,563 aligned nucleotides from four protein-coding regions. The LAm B clade appears to be divided into at least two highly supported clades, which are geographically restricted to either Colombia/Argentina or Brazil respectively. Moreover, a complex population genetic structure was identified within LAm A clade supporting multiple monophylogenetic species, which could be driven by rapid host or environmental adaptation (~0.5 MYA). We found two divergent clades, which include Latin American isolates (newly named as LAm A1 and LAm A2), harboring a cryptic cluster in association with bats.Conclusions/SignificanceAt least six new phylogenetic species are proposed in the Histoplasma species complex supported by different phylogenetic and population genetics methods, comprising LAm A1, LAm A2, LAm B1, LAm B2, RJ and BAC-1 phylogenetic species. The genetic isolation of Histoplasma could be a result of differential dispersion potential of naturally infected bats and other mammals. In addition, the present study guides isolate selection for future population genomics and genome wide association studies in this important pathogen complex.

show abstract

“…Additional molecular studies revealed further differences between the populations of H. capsulatum. Taylor et al [15] using sequence polymorphism of a 240-nt fragment of the (GA) n microsatellite and its flanking regions, found two major clusters within H. capsulatum, and also showed an association between genetic diversity and the geographic distribution.…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of Histoplasma capsulatum using rolling circle amplification

Furuie

Sun

Nascimento

et al. 2015

Mycoses

Self Cite

View full text Add to dashboard Cite

Histoplasmosis is a systemic fungal disease that occurs worldwide, causing symptomatic infection mostly in immunocompromised hosts. Etiological agent is the dimorphic fungus, Histoplasma capsulatum, which occurs in soil contaminated with bird or bat droppings. Major limitation in recognition of H. capsulatum infections is the low awareness, since other diseases may have similar symptomatology. The molecular methods have gained importance because of unambiguous diagnostic ability and efficiency. The aim of this study was to develop and evaluate a padlock probe in view of rolling circle amplification (RCA) detection method which targets ITS (Internal Transcribed Spacer) rDNA of H. capsulatum enabling rapid and specific detection of the fungus in clinical samples. Two padlock probes were designed and one of these (HcPL2) allowed specific amplification of H. capsulatum DNA while no cross-reactivity was observed with fungi used as negative controls. This method proved to be effective for H. capsulatum specific identification and demonstrated to be faster than the traditional method of microbiological identification.

show abstract

Genetic diversity of Histoplasma capsulatum isolated from infected bats randomly captured in Mexico, Brazil, and Argentina, using the polymorphism of (GA)n microsatellite and its flanking regions

Cited by 21 publications

References 40 publications

Frequency and Genetic Diversity of the MAT1 Locus of Histoplasma capsulatum Isolates in Mexico and Brazil

Frequency and Genetic Diversity of the MAT1 Locus of Histoplasma capsulatum Isolates in Mexico and Brazil

Worldwide Phylogenetic Distributions and Population Dynamics of the Genus Histoplasma

Molecular identification of Histoplasma capsulatum using rolling circle amplification

Contact Info

Product

Resources

About