Genetic diversity of hop stunt viroid from symptomatic and asymptomatic citrus trees in Iran

Abstract

Hop stunt viroid as the causal agent of cachexia disease has detected from citrus trees in different areas in Iran. Although cachexia has not been reported as a decline disease for citrus trees, it can impair crop quality and reduce plant yields. This study was undertaken to molecularly detect HSVd among different commercial citrus cultivars and determine genetic diversity of this viroid in Mazandaran province of Iran. Sampling was performed from symptomatic and symptomless citrus cultivars in Mazandaran province. HSVd specific primers were used for molecular detection. SSCP and sequencing were applied to assay HSVd genetic diversity. Results showed the detection of HSVd in all symptomatic Satsuma (25 out of 25), Clementine (25 out of 25), sweet lime (20 out of 20) and sweet orange cv. Valencia (7 out of 7), as well as, 31% (14 out of 22), 100% (12 out of 12) and 33% (5 out of 15) of page mandarin, lemon and grapefruit trees, respectively. 10 different HSVd genomes were identified by sequencing the SSCP profiles among which HSVd‐IR1 had the most frequency.

Enfermedades emergentes y reemergentes de plantas en Latinoamérica: una revisión.

“…En la misma vía, el viroide del enanismo del lúpulo (HSVd) es de importancia en cultivos de cítricos en Latinoamérica, ha sido reportado en Cuba y es transmitido por material agrícola contaminado o lesiones causada por áfidos en cítricos como el toronjo (Soto et al, 2008). Además de cítricos (Rutaceae), el HSVd se ha identificado en un amplio rango de familias de plantas (Moraceae, Rosaceae, Anacardiaceae y Malvaceae) (Maddahian et al, 2019;Luigi et al, 2013;Vamenani et al, 2019). En la tabla 5, se presentan algunos primeros reportes para especies de virus y viroides causando enfermedad en plantas en Latinoamérica.…”

Sensitivity and applications of the PCR Single-Strand Conformation Polymorphism method

“…PCR-SSCP could be used for the authentication of snapper species by SSCP [ 48 ], the population genetic structure of zeugodacus tau species complex [ 49 ], for the genetic diversity of citrus trees [ 50 ], identification of phytophthora genes polymorphism of potato [ 51 ], identification of genetic diversity of lemon and mandarin varieties [ 52 ], detection of the genetic variation of alfalfa gene [ 53 ], prediction of the chloroplast gene polymorphism in barley varieties [ 54 ] and bacteria identification in wood tick [ 55 ].…”

Pospiviroides de importância para hortaliças e ornamentais: panorama dos aspectos fitopatológicos e dos riscos para os sistemas agrícolas brasileiros

“…e uma gama ampla de hospedeiras incluindo frutíferas lenhosas das famílias Anacardiaceae (pistache) (MADDAHIAN et al 2019), Moraceae (amoreira e figueira), Rosaceae (ameixeira, amendoeira, cerejeira, damasco, macieira, pereira e pessegueiro), Rutaceae (Citrus spp.) (HATAYA et al 2017;VAMENANI et al 2019) e Vitaceae (videira) (SANO et al 1985;EIRAS et al 2006b). Com base em análises filogenéticas, foi proposto que o HSVd isolado de lúpulo teve sua origem em videira (SANO et al 2001;KAWAGU-CHI-ITO et al 2009).…”