Genetic diversity of human isolates of Salmonella enterica serovar Enteritidis in Malaysia

Bakeri, Shamsilawani Ahamed; Yasin, Rohani Md; Koh, Y.T.; Puthucheary, S. D.; Thong, Kwai Lin

doi:10.1046/j.1365-2672.2003.02033.x

Cited by 21 publications

(20 citation statements)

References 29 publications

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“…Enteritidis plasmid of size 55 kb is commonly found among the majority of Salm. Enteritidis isolates and has been denoted the serovar‐specific plasmid (Bakeri et al. 2003).…”

Section: Discussionmentioning

confidence: 99%

Salmonella enterica isolates from pasture-raised poultry exhibit antimicrobial resistance and class I integrons

Melendez

Hanning

Han

et al. 2010

Journal of Applied Microbiology

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Aims: While considerable foodborne pathogen research has been conducted on conventionally produced broilers and turkeys, few studies have focused on free‐range (organic) or pastured poultry. The current surveillance study was designed to isolate, identify and genetically characterize Salmonella from pastured poultry farm environment and from retail samples. Methods and Results: In this study, 59 isolates were collected from two pastured poultry farms (n = 164; pens, feed, water and insect traps) and retail carcasses (n = 36) from a local natural foods store and a local processing plant. All isolates were serotyped and analysed phenotypically (antimicrobial resistance profiles) and genotypically (DNA fingerprints, plasmid profiles and integron analysis). Salmonella enterica was detected using standard microbiological methods. Salmonella Kentucky was the most prevalent serotype detected from the sampled sources (53%), followed by Salmonella Enteritidis (24%), Bareilly (10%), Mbandaka (7%), Montevideo (5%) or Newport (2%). All isolates were resistant to sulfisoxazole and novobiocin, and the majority (40/59) possessed class I integrons shown by PCR detection. Each Salmonella serotype elicited a distinct pulsed‐field gel electrophoresis fingerprint profile, and unique differences were observed among the serotypes. Conclusions: The findings of this study show that Salmonella serotypes isolated from pasture‐raised poultry exhibit antimicrobial resistance and class I integrons. Significance and Impact of the Study: This study demonstrates that despite the cessation of antibiotic usage in poultry production, antibiotic resistant Salmonella may still be recovered from the environment and poultry products.

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“…Enteritidis plasmid of size 55 kb is commonly found among the majority of Salm. Enteritidis isolates and has been denoted the serovar‐specific plasmid (Bakeri et al. 2003).…”

Section: Discussionmentioning

confidence: 99%

Salmonella enterica isolates from pasture-raised poultry exhibit antimicrobial resistance and class I integrons

Melendez

Hanning

Han

et al. 2010

Journal of Applied Microbiology

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“…Salmonellosis is caused by two species of Salmonella (Salmonella enterica and Salmonella bongori), which are etiological agents for diarrhoeal and systemic infection in humans. Human infections with non-typhoidal Salmonella (NTS) are frequently associated with the consumption of contaminated food [1]. This can result in diarrhoeal disease, bacteraemia and extraintestinal focal infection in infants such as meningitis, and may also result in more serious complications among the elderly and immunocompromised patients [2].…”

Section: Introductionmentioning

confidence: 99%

Molecular Epidemiology of Nontyphoidal Salmonella in Poultry and Poultry Products in India: Implications for Human Health

et al. 2015

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Human infections with non-typhoidal Salmonella (NTS) serovars are increasingly becoming a threat to human health globally. While all motile Salmonellae have zoonotic potential, Salmonella Enteritidis and Salmonella Typhimurium are most commonly associated with human disease, for which poultry are a major source. Despite the increasing number of human NTS infections, the epidemiology of NTS in poultry in India has not been fully understood. Hence, as a first step, we carried out epidemiological analysis to establish the incidence of NTS in poultry to evaluate the risk to human health. A total of 1215 samples (including poultry meat, tissues, egg and environmental samples) were collected from 154 commercial layer farms from southern India and screened for NTS. Following identification by cultural and biochemical methods, Salmonella isolates were further characterized by multiplex PCR, allele-specific PCR, enterobacterial repetitive intergenic consensus (ERIC) PCR and pulse field gel electrophoresis (PFGE). In the present study, 21/1215 (1.73 %) samples tested positive for NTS. We found 12/392 (3.06 %) of tissue samples, 7/460 (1.52 %) of poultry products, and 2/363 (0.55 %) of environmental samples tested positive for NTS. All the Salmonella isolates were resistant to oxytetracycline, which is routinely used as poultry feed additive. The multiplex PCR results allowed 16/21 isolates to be classified as S. Typhimurium, and five isolates as S. Enteritidis. Of the five S. Enteritidis isolates, four were identified as group D Salmonella by allele-specific PCR. All of the isolates produced different banding patterns in ERIC PCR. Of the thirteen macro restriction profiles (MRPs) obtained by PFGE, MRP 6 was predominant which included 6 (21 %) isolates. In conclusion, the findings of the study revealed higher incidence of contamination of NTS Salmonella in poultry tissue and animal protein sources used for poultry. The results of the study warrants further investigation on different type of animal feed sources, food market chains, processing plants, live bird markets etc., to evaluate the risk factors, transmission and effective control measures of human Salmonella infection from poultry products.

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“…Such mutation may remove or create recognition sites through insertion, deletion, translocation and inversion, or by mobile genetic elements. The assumption is that as time passes and organisms spread, divergence may occur [4]. This study also showed that both PFGE and phage typing were capable of differentiating S. Enteritidis strains.…”

Section: Discussionmentioning

confidence: 55%

Characterization of <i>Salmonella</i> spp. Isolated from an Integrated Broiler Chicken Operation in Korea

Lee

Kim

Park

et al. 2007

The Journal of Veterinary Medical Science

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ABSTRACT. The purpose of this study was to investigate the biological and genetic characterization of persistent Salmonella isolates in an integrated broiler chicken operation, in an attempt to elucidate the source of contamination. From the breeder farm, the hatchery, the broiler farm and the chicken slaughter house of an integrated broiler chicken operation, a total of 6 serotypes were observed. Although S. Heidelberg was not detected in the broiler farm, it was consistently found in the breeder farm, the hatchery and the chicken slaughter house. Also, S. Enteritidis and S. Senftenberg were found in the hatchery and the chicken slaughter house, and the hatchery and the broiler farm, respectively. S. Gallinarum and S. Blockley were found only in the broiler farm, and S. Virchow was only recovered in the chicken slaughter house. Isolated S. Heidelberg, S. Enteritidis and S. Senftenberg strains were divided into 3, 5 and 7 types, respectively, on the basis of all properties. Especially, S. Senftenberg isolates, divided into four types by their antimicrobial resistance patterns, were all obviously the XbaI PFGE pattern. Also, four S. Enteritidis isolates resistant to nalidixic acid showed a difference in phage type and PFGE pattern. Such a different pattern was shown despite Salmonella isolates originating from an integrated broiler operation, suggesting that further epidemiological studies on many integrated chicken companies in Korea are needed. KEY WORDS: integrated broiler chicken operation, Salmonella.

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Genetic diversity of human isolates of Salmonella enterica serovar Enteritidis in Malaysia

Cited by 21 publications

References 29 publications

Salmonella enterica isolates from pasture-raised poultry exhibit antimicrobial resistance and class I integrons

Salmonella enterica isolates from pasture-raised poultry exhibit antimicrobial resistance and class I integrons

Molecular Epidemiology of Nontyphoidal Salmonella in Poultry and Poultry Products in India: Implications for Human Health

Characterization of <i>Salmonella</i> spp. Isolated from an Integrated Broiler Chicken Operation in Korea

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