Genetic Diversity of Parkia biglobosa from Different Agroecological Zones of Nigeria Using RAPD Markers

Abstract

Parkia biglobosa (Jacq.) is an important leguminous tree crop in the African Savannahs useful to the natives where it is found, for domestic use. Previous diversity studies on this tree crop had been majorly on morphological and biochemical analysis. In order to capture the maximum diversity not obtained by previous research, the study aimed at evaluating the genetic diversity of accessions of this crop in the different agroecological zones in Nigeria using RAPD markers. A total of 81 scorable bands with an average of 8.1 bands per primer were amplified among the accessions studied. Intrazonal genetic diversity analysis showed a percentage polymorphism with a range of 11.11% to 65.43% among the agroecological zones studied. Although, gene diversity was highest within Humid forest agroecological zone, a low genetic distance and high genetic similarity between the agroecological zones were observed. Cluster analysis indicated six main groups of which four groups had single accessions while the two groups clustered the remaining accessions, indicating a narrowed genetic base from the 23 accessions studied.

Agronomic Practices, Genetic Diversity and Population Structure of <em>Moringa oleifera</em> (Lam.) in Nigeria

“…This pattern of genetic diversity has been reported among M. oleifera populations using SSR markers [8] even though the accessions of the populations were raised in the same environment of similar climatic and soil conditions. The low genetic divergence among the populations (5 %) and higher genetic differentiation within the populations (95 %) recorded in this study have been reported from previous studies which also identified a higher degree of genetic dissimilarities within-population compared to lower genetic diversity among the populations [8,43]. This trend of result can be attributed to the reproductive biology and pollination mechanisms of the species as an outcrossing and autogamous tree species with a higher rate of gene flow via pollen grains and exchange of propagules with nearby populations.…”

Phylogeography of African Locust Bean (Parkia biglobosa) Reveals Genetic Divergence and Spatially Structured Populations in West and Central Africa

“…Data on the occurrence of P. biglobosa were compiled from the following sources: seed stands database (CNSF, Burkina Faso), data from the Herbarium Senckenbergianum (Germany), data from the herbarium of Aarhus University (Denmark), the West African Vegetation Database ( Schmidt et al 2012 ), georeferenced photo records of African plants ( Dressler et al 2014 ), observation records from a transect study in western Burkina Faso ( Heubes 2012 ), georeferenced point locations from a detailed distribution map in Nigeria ( Amusa et al 2014 ) and point locations obtained from the Global Biodiversity Information Facility ( GBIF 2017 ). In total, 2032 occurrence records from 14 African countries (Benin, Burkina Faso, Cameroon, Central African Republic, Chad, Ghana, Guinea, Guinea-Bissau, Ivory Coast, Mali, Niger, Nigeria, Senegal, and Togo) were collated.…”

Genetic conservation in Parkia biglobosa (Fabaceae: Mimosoideae) - what do we know?

“…There are very few marker-based studies at hand assessing genetic diversity of P. biglobosa. A study using Random Amplified Polymorphic DNA (RAPD) markers revealed a weak genetic diversity (H E =0.05-0.18) in 23 open-pollinated families of P. biglobosa in Nigeria (Amusa et al, 2014). However, due to the small scale of the sampling, meaningful conclusions for the whole species cannot be drawn.…”