Genetic Diversity Of Plukenetia Volubilis L. Assessed By ISSR Markers^*

Abstract

The diversity and genetic relationships in 173 sacha inchi samples were analyzed using ISSR markers. Thirty ISSR primers were used, only 8 showed variability in tested samples. ISSR fragments ranged from 200 to 2500 bp. The mean number of bands per primer was 12 and the average number of polymorphic bands per primer was 11. The lowest percentages of polymorphic bands (27%), gene diversity (0.103), and Shannon’s information index (0.15) were exhibited by the Santa Lucia population, which was also geographically most distant. This fact may be attributed to a very small size of this group. In contrast, the Dos de Mayo population exhibited the highest percentage of polymorphic bands (78%), and the Santa Cruz population the highest Nei’s gene diversity index (0.238) and Shannon’s information index (0.357). The obtained level of genetic variability was 36% among tested populations and 64% within populations. Although the diversity indices were low, a cluster analysis revealed 8 clusters containing mainly samples belonging to individual populations. Principal coordinate analysis clearly distinguished Chumbaquihui, Pucallpa, Dos de Mayo, and Aguas de Oro populations, the others were intermixed. The obtained results indicated the level of genetic diversity present in this location of Peru, although it is influenced by anthropological aspects and independent on the geographical distances.

Molecular genetic diversity and mating system in sacha inchi progenies1

“…The total number of polymorphic fragments varied from 70 to 104, with an average of approximately 84 fragments per primer combination. In a study of the same species, Ocelák et al (2015), assessing 173 sacha inchi accessions using ISSR markers, observed an average of only eleven polymorphic fragments per primer combination. Also studying sacha inchi, Rodrigues et al (2013) obtained 191 polymorphic fragments using four AFLP primers combinations.…”

Towards a productive model for the Sacha Inchi value chain: A scientometric approach

“…It includes most of the research topics required to design specific production models . The most outstanding practices are agroforestry systems, vegetative propagation (Cachique et al, 2018), phylogenetic relationships (Ocelák et al, 2015), seedling growth (Rodrigues et al, 2014;Tian et al, 2013), floral development (Fu et al, 2018a), and the use of growth promoters (Pezo et al, 2019). In addition, the genetic analysis of seeds and seedlings through transcriptomic sequencing (Liu et al, 2020) (Vitar et al, 2023); and combined used of NPK fertilizers and biochar (Suraya et al, 2023).…”

The Complete Chloroplast Genome of Plukenetia volubilis Provides Insights Into the Organelle Inheritance

“…Thanks to all of the above, the Sacha inchi industry has experienced a great demand in tropical countries, among others, Colombia, Peru, Ecuador, and Brazil, where the cultivation of this species has been increasing (Valente et al, 2017), without developed varieties and ecotypes with genetic stability. In those countries, the ecotypes have been rudimentary and are too difficult to track because of the lack of studies that have been made to understand its genetic diversity (Ocelák et al, 2015). This lack of studies makes it necessary to develop molecular mechanisms that characterize the species, recognize ecotypes, and generate successful seed marketing; responses can be faced by knowing the Sacha inchi chloroplast genome.…”