Genetic diversity of imported PRRSV-2 strains, 2005–2020, Hungary

Jakab, Szilvia; Kaszab, Eszter; Marton, Szilvia; Bányai, Krisztiàn; Bálint, Ádám; Nemes, Imre; Szabó, István

doi:10.3389/fvets.2022.986850

Cited by 8 publications

(9 citation statements)

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“…Hungary became a net import country of prefatteners, and the main suppliers for this were the Netherlands, Denmark and Germany. Similar findings were reported for PRRSV-2 in the same study period [ 44 ]. To reduce this tendency, the National Chief Veterinary has been regulating the importing practice, and from 2017, purchased pigs settled in for fattening must come from certified PRRS-free herds.…”

Section: Discussionsupporting

confidence: 90%

Spatiotemporal Distribution of PRRSV-1 Clades in Hungary with a Focus on the Era of Disease Eradication

Bálint,

Jakab,

Kaszab

et al. 2024

Animals

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Porcine reproductive and respiratory syndrome (PRRS) is the cause of the most severe economic losses in the pig industry worldwide. PRRSV is extremely diverse in Europe, which poses a significant challenge to disease control within a country or any region. With the combination of phylogenetic reconstruction and network analysis, we aimed to uncover the major routes of the dispersal of PRRSV clades within Hungary. In brief, by analyzing >2600 ORF5 sequences, we identified at least 12 clades (including 6 clades within lineage 1 and 3 clades within lineage 3) common in parts of Western Europe (including Denmark, Germany and the Netherlands) and identified 2 novel clades (designated X1 and X2). Of interest, some genetic clades unique to other central European countries, such as the Czech Republic and Poland, were not identified. The pattern of PRRSV clade distribution is consistent with the route of the pig trade among countries, showing that most of the identified clades were introduced from Western Europe when fatteners were transported to Hungary. As a result of rigorous implementation of the national eradication program, the swine population was declared officially free from PRRSV. This map of viral diversity and clade distribution will serve as valuable baseline information for the maintenance of PRRSV-free status in the post-eradication era.

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Section: Discussionsupporting

confidence: 90%

Spatiotemporal Distribution of PRRSV-1 Clades in Hungary with a Focus on the Era of Disease Eradication

Bálint,

Jakab,

Kaszab

et al. 2024

Animals

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“…PRRSV strains are classified into two genotypes (PRRSV-1 and PRRSV-2) and the two genotype strains share nearly 60% nucleotide sequence homology [ 8 ]. PRRSV-2 has been widely prevalent in the world, and is further divided into nine lineages based on the genetic features of the ORF5 gene [ 9 , 10 , 11 ]. Four of the nine PRRSV-2 lineages have been found to be prevalent in Chinese pig herds: lineage 1 (sublineage 1.8, NADC30-like strains and sublineage 1.5, NADC34-like strains), lineage 3 (QYYZ-like strains), lineage 5 (VR2332-like strains), and lineage 8 (sublineage 8.1, Chinese classical PRRSV strains and sublineage 8.7, Chinese highly pathogenic PRRSV strains (HP-PRRSV)) [ 3 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Epidemiology and Genetic Characteristics of Porcine Reproductive and Respiratory Syndrome Virus in the Hunan and Hebei Provinces of China

Zhai

Yu²,

Zhang

et al. 2023

Veterinary Sciences

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Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant threat to the pig industry in China. However, the epidemiological characteristics of PRRSV after the outbreak of African swine fever in China were not thoroughly investigated. In the present study, the serological and epidemiological investigations of PRRSV in pigs from the Hunan and Hebei provinces of China were assessed. The results showed that 73.12% (95% CI 71.74–74.49) of pigs were positive for PRRSV-special antibody by enzyme-linked immunosorbent assay. Out of 5799 samples, 482 (8.31%, 95% CI 7.60–9.02) samples were positive for PRRSV nucleic acids. The positive rates of PRRSV in healthy pigs from farms and slaughterhouses were 2.27% (47/2072) and 7.70% (217/2818), which were lower than that in diseased pigs (23.98%, 218/909). Furthermore, the full-length OFR5 gene sequences of 43 PRRSV strains were sequenced and analysed. Phylogenetic analysis revealed that 43 isolates were classified into three lineages, namely lineage 1 (n = 24), lineage 8 (n = 15), and lineage 3 (n = 4). Lineage 1 could be further divided into sublineage 1.5 (n = 2) and sublineage 1.8 (n = 22), and lineage 8 was classified into sublineage 8.1 (n = 3) and sublineage 8.7 (n = 12). Collectively, our findings revealed the severe prevalence of PRRSV in the Hunan and Hebei provinces, where sublineage 1.8 and sublineage 8.7 predominated. The present study provides the update information of the epidemiological and genetic characteristics of PRRSV in the investigated regions, which will be beneficial for PRRS control.

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“…Because of their great diversity, ORF5 (GP5) and NSP2 are frequently employed in phylogenetic and evolutionary analyses [ 18 , 19 ]. The ORF5 gene is considered for the global PRRSV classification system, which divides PRRSV-1 into subtypes 1–4, PRRSV-2 into lineages L1–9, and several sub-lineages [ 20 , 21 ]. Subtype 1 is further subdivided into subgroup A, B, and C (subtypes 1C, 1B, and 1C) [ 14 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Porcine Reproductive and Respiratory Syndrome Virus in Korea from 2018 to 2022

et al. 2023

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Porcine reproductive and respiratory syndrome (PRRS) is an endemic disease in the Republic of Korea. Surveillance of PRRS virus (PRRSV) types is critical to tailor control measures. This study collected 5062 serum and tissue samples between 2018 and 2022. Open reading frame 5 (ORF5) sequences suggest that subgroup A (42%) was predominant, followed by lineage 1 (21%), lineage 5 (14%), lineage Korea C (LKC) (9%), lineage Korea B (LKB) (6%), and subtype 1C (5%). Highly virulent lineages 1 (NADC30/34/MN184) and 8 were also detected. These viruses typically mutate or recombine with other viruses. ORF5 and non-structural protein 2 (NSP2) deletion patterns were less variable in the PRRSV-1. Several strains belonging to PRRSV-2 showed differences in NSP2 deletion and ORF5 sequences. Similar vaccine-like isolates to the PRRSV-1 subtype 1C and PRRSV-2 lineage 5 were also found. The virus is evolving independently in the field and has eluded vaccine protection. The current vaccine that is used in Korea offers only modest or limited heterologous protection. Ongoing surveillance to identify the current virus strain in circulation is necessary to design a vaccine. A systemic immunization program with region-specific vaccinations and stringent biosecurity measures is required to reduce PRRSV infections in the Republic of Korea.

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Genetic diversity of imported PRRSV-2 strains, 2005–2020, Hungary

Cited by 8 publications

References 50 publications

Spatiotemporal Distribution of PRRSV-1 Clades in Hungary with a Focus on the Era of Disease Eradication

Spatiotemporal Distribution of PRRSV-1 Clades in Hungary with a Focus on the Era of Disease Eradication

Epidemiology and Genetic Characteristics of Porcine Reproductive and Respiratory Syndrome Virus in the Hunan and Hebei Provinces of China

Molecular Characterization of Porcine Reproductive and Respiratory Syndrome Virus in Korea from 2018 to 2022

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