Genetic diversity of Leishmania infantum field populations from Brazil

Segatto, Marcela; Ribeiro, Lucas S.; Costa, Dorcas Lamounier; Costa, Carlos Henrique Nery; Oliveira, Márcia Rosa de; Carvalho, Sílvio Fernando Guimarães; Macedo, Andréa Mara; Valadares, Helder Magno Silva; Dietze, Reynaldo; Brito, Cristiana Ferreira Alves de; Lemos, Elenice Moreira

doi:10.1590/s0074-02762012000100006

Cited by 34 publications

(38 citation statements)

References 37 publications

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“…16 None of the seven microsatellite markers designed for the MLMT analysis amplified any DNA samples from L. amazonensis or L. braziliensis, which confirms the specificity of these primers to the L. donovani complex. Five of these seven markers were shown to be monomorphic.…”

Section: Discussionmentioning

confidence: 76%

“…17,30 Each polymerase chain reaction (PCR) was performed using fluorescent primers, 3 ng DNA target, and a stepdown protocol as described earlier. 16 The allele sizes were determined in a 6% denaturing polyacrylamide gel using an Automatic Laser Fluorescent (ALF) sequencer (GE Healthcare, Milwaukee, WI). Images were processed using the AlleleLocator software (GE Healthcare, Milwaukee, WI).…”

Section: Methodsmentioning

confidence: 99%

“…The findings in the work by Alonso and others 15 are consistent with the results of the present study in relation to the genomic DNA of L. infantum strains from a single endemic region in Brazil. 15 Similarly, the work by Segatto and others 16 also described a low degree of polymorphism among human L. infantum isolates from different geographical regions in Brazil when employing both RAPD and MLMT techniques.…”

mentioning

confidence: 88%

“…Two studies identified a low degree of genetic variability among human L. infantum isolates from distinct geographic regions in Brazil. 15,16 In addition, a recent study performed a continent-wide analysis of New World L. infantum population structure and showed that Brazilian L. infantum strains belonged to the Old World MON-1 zymodeme, indicating a recent introduction of this parasite into the New World. 17 However, only few canine isolates have been analyzed in these previous studies.…”

Section: Introductionmentioning

confidence: 99%

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An Assessment of the Genetic Diversity of Leishmania infantum Isolates from Infected Dogs in Brazil

Batista

Segatto²,

Guedes³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Correlations between the genetic diversity of Leishmania infantum (syn. L. chagasi) isolates and their respective geographic origins support the theoretic assumption that visceral leishmaniasis probably originated in the Old World. Because dogs are widely considered to be the main reservoir of this disease, the present study aimed to investigate the degree of genetic divergence among 44 leishmanial canine isolates from two Brazilian cities, Jequié and Campo Grande, located approximately 2,028 km from each other. We hypothesized that a low degree of genetic divergence would be observed among these isolates. In fact, statistical analyses found no significant differences between the isolates using both random amplified polymorphic DNA and multilocus microsatellite typing genotyping techniques with three and seven markers, respectively. These findings provide support for the recent introduction of L. infantum into the New World.

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Section: Discussionmentioning

confidence: 76%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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An Assessment of the Genetic Diversity of Leishmania infantum Isolates from Infected Dogs in Brazil

Batista

Segatto²,

Guedes³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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“…Notably, the other two VLh90 isolates (3VLh90 and 5VLh90) were always associated, in the same analyses, with the most recent asymptomatic human, visceral human and dog isolates. The close relationship between L. infantum strains obtained from dogs and humans is well documented in Brazilian isolates (Segatto et al, 2012) as well as in other countries where leishmaniasis outbreaks occur frequently (Kuhls et al, 2011). Thus, when the nucleotide diversity, principal components and phylogenetic analysis are considered collectively, it is possible to suggest that during the 1990s there were at least two different lineages of L. infantum isolates giving rise to most of the isolates in the current study.…”

Section: Discussionmentioning

confidence: 96%

Comparative analyses of whole genome sequences of Leishmania infantum isolates from humans and dogs in northeastern Brazil

Teixeira

Monteiro

Martins

et al. 2017

International Journal for Parasitology

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The genomic sequences of 20 Leishmania infantum isolates collected in northeastern Brazil were compared with each other and with the available genomic sequences of 29 L. infantum/donovani isolates from Nepal and Turkey. The Brazilian isolates were obtained in the early 1990s or since 2009 from patients with visceral or non-ulcerating cutaneous leishmaniasis, asymptomatic humans, or dogs with visceral leishmaniasis. Two isolates were from the blood and bone marrow of the same visceral leishmaniasis patient. All 20 genomic sequences display 99.95% identity with each other and slightly less identity with a reference L. infantum genome from a Spanish isolate. Despite the high identity, analysis of individual differences among the 32 million base pair genomes showed sufficient variation to allow the isolates to be clustered based on the primary sequence. A major source of variation detected was in chromosome somy, with only four of the 36 chromosomes being predominantly disomic in all 49 isolates examined. In contrast, chromosome 31 was predominantly tetrasomic/pentasomic, consistent with its regions of synteny on two different disomic chromosomes of Trypanosoma brucei. In the Brazilian isolates, evidence for recombination was detected in 27 of the 36 chromosomes. Clustering analyses suggested two populations, in which two of the five older isolates from the 1990s clustered with a majority of recent isolates. Overall the analyses do not suggest individual sequence variants account for differences in clinical outcome or adaptation to different hosts. For the first known time, DNA of isolates from asymptomatic subjects were sequenced. Of interest, these displayed lower diversity than isolates from symptomatic subjects, an observation that deserves further investigation with additional isolates from asymptomatic subjects.

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