Genetic diversity of Leishmania tropica: Unexpectedly complex distribution pattern

Charyyeva, Arzuv; Çetinkaya, Ülfet; Ozkan, Bora; Şahin, Serkan; Yaprak, Nermin; Şahi̇n, İzzet; Yurchenko, Vyacheslav; Kostygov, Alexei Y.

doi:10.1016/j.actatropica.2021.105888

Cited by 13 publications

(7 citation statements)

References 33 publications

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“…Leishmaniasis is a parasitic infection caused by protozoa of the genus Leishmania spp. representing a serious public health problem in many countries of the world, affecting socially and economically vulnerable populations [ 51 ]. In the study of Luz [ 52 ], a comparative analysis of the in vitro activities of the single usnic acid from C. substellata and potassium usnate against the promastigotes of Leishmania (Leishmania) infantum chagasi was carried out, describing the morphological changes of the parasites through SEM, in addition, to assess the cytotoxicity on macrophages (RAW 264.7) for both drugs after exposure.…”

Section: Molluscicidal and Antiparasitic Activitiesmentioning

confidence: 99%

The Natural Compound Hydrophobic Usnic Acid and Hydrophilic Potassium Usnate Derivative: Applications and Comparisons

et al. 2021

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Usnic acid is the best-studied lichen metabolite, presenting several biological activities, such as antibacterial, immunostimulating, antiviral, antifungal, anti-inflammatory, and antiparasitic agents; despite these relevant properties, it is a hydrophobic and toxic molecule. In this context, scientific research has driven the development of innovative alternatives, considering usnic acid as a source of raw material in obtaining new molecules, allowing structural modifications (syntheses) from it. The purpose is to optimize biological activities and toxicity, with less concentration and/or response time. This work presents a literature review with an analogy of the hydrophobic molecule of usnic acid with its hydrophilic derivative of potassium usnate, emphasizing the elucidation and structural characteristics, biological activities, and toxicological aspects of both molecules, and the advantages of using the promising derivative hydrophilic in different in vitro and in vivo assays when compared to usnic acid.

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Section: Molluscicidal and Antiparasitic Activitiesmentioning

confidence: 99%

The Natural Compound Hydrophobic Usnic Acid and Hydrophilic Potassium Usnate Derivative: Applications and Comparisons

et al. 2021

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“…Infection with these species may also present skin manifestations in the cases of post-kala-azar dermal leishmaniasis or atypical leishmaniases (Guan et al ., 2013 ; Zhang et al ., 2014 ; Ben-Shimol et al ., 2016 ; Zijlstra, 2016 ). Leishmania aethiopica , L. major and L. tropica mostly cause CL, although some isolates of L. major and L. tropica were occasionally identified from patients with VL (Alborzi et al ., 2008 ; Bruschi and Gradoni, 2018 ; Charyyeva et al ., 2021 ). The CL can be further subdivided into anthroponotic (ACL) and zoonotic (ZCL) forms, which are predominantly caused by L. tropica and L. major , respectively (Akilov et al ., 2007 ; Ghatee et al ., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Revisiting epidemiology of leishmaniasis in central Asia: lessons learnt

et al. 2022

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In this work we reviewed historical and recent data on Leishmania spp. infection combining data collected in Turkmenistan, Uzbekistan, Kazakhstan, Kyrgyzstan, Iran, China and Mongolia. We specifically focused on a complex of co-existing species (Leishmania major, Leishmania turanica and Leishmania gerbilli) sharing the same animal reservoirs and vectors. In addition, we analysed the presence of dsRNA viruses in these species and discussed future research directions to identify species-specific traits, which may determine susceptibility of different Leishmania spp. to viral infection.

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“…ITSs are removed from the primary transcript by snoRNAs and multiple enzymes [ 23 , 24 ]. Analysis of available bioinformatics databases has revealed different subunits of the rRNAs of L. infantum located in different chromosomes and are not arranged in a head-to-tail tandem array.…”

Section: Discussionmentioning

confidence: 99%

The efficacy of AuNP-probe conjugate nanobiosensor in non-amplification and amplification forms for the diagnosis of leishmaniasis

et al. 2022

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Nanobiosensor platforms have emerged as convenient and promising approaches with remarkable efficacy for the diagnosis of infectious diseases. Gold nanoparticles (AuNPs) have been widely used due to numerous advantageous properties such as optical, electrical, physicochemical and great biomolecules binding capabilities. This study aimed to apply AuNP-Probe Conjugate for the detection of Leishmania spp., using colorimetric and amplification methods targeting parasitic ITS2 fragment. The first method was carried out by hybridization of 10µL of DNA with 4 µL of probe and addition of 5 µL of 0.2 N HCl (non-amplification method). Second method was followed by polymerase chain reaction (PCR) amplification using thiolated primer, 5 µL of AuNP and 5 µL of 0.2 N HCl. The appearance of red and purple colors indicated positive and negative results, respectively. The minimum of detection for non-amplification and amplification methods for three strains of Leishmania namely L. major, L. tropica and L. infantum were determined to be 32 fg/µL and 16 fg/µL, respectively. Sensitivity for detection of visceral leishmaniasis (VL) for non-amplification and amplification methods included 96% and 100%, respectively and for cutaneous leishmaniasis (CL) included 98% and 100%, respectively. The results of this investigation revealed that sensitivity of amplification method was the same as RT-qPCR, while that of non-amplification method was lower. However, this method was promising because of no need for any equipment, high specificity, enough sensitivity, low cost and rapidity (less than 30 min) to complete after genomic DNA extraction.

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Genetic diversity of Leishmania tropica: Unexpectedly complex distribution pattern

Cited by 13 publications

References 33 publications

The Natural Compound Hydrophobic Usnic Acid and Hydrophilic Potassium Usnate Derivative: Applications and Comparisons

The Natural Compound Hydrophobic Usnic Acid and Hydrophilic Potassium Usnate Derivative: Applications and Comparisons

Revisiting epidemiology of leishmaniasis in central Asia: lessons learnt

The efficacy of AuNP-probe conjugate nanobiosensor in non-amplification and amplification forms for the diagnosis of leishmaniasis

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