Genetic diversity of liver flukes (Fasciola hepatica) from Eastern Europe

Teofanova, Denitsa; Kantzoura, Vaia; Walker, Stephen; Radoslavov, Georgi; Hristov, Peter; Théodoropoulos, Georgios; Bankov, Ilia; Trudgett, Alan

doi:10.1016/j.meegid.2010.10.002

Cited by 26 publications

(55 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is a common casus finding of additional and not depended on the distribution of the basic lineages genotype groups (e.g. S28f and S28e; Teofanova et al, 2011) which can not be previously predicted and more hardly to explain with a higher level of speculation.…”

Section: S Rdna Gene Regions Analysismentioning

confidence: 86%

“…Noncoding regions of mtDNA (long (LNR) and short (SNR)) have been studied (Korchagina et al, 2009) and it has been assumed that the polymorphisms and structural differences among them are associated with different haplotype groups of F. hepatica. In comparison with all other researchers, Teofanova et al (2011 and Kantzoura et al(2011), performed the investigations not with commonly used nad1 and cox1 genes from respectively second and third "plastic" regions of mtDNA ( Figure 3) but with part from the first one which found to be most variable. Teofanova et al (2011) have defined the population structure of liver fluke in Eastern Europe and have studied population dynamics of F. hepatica and genetic diversity of its populations in the Netherlands.…”

Section: Mitochondrial Dna Regions Used For Snp Assaymentioning

confidence: 94%

“…In comparison with all other researchers, Teofanova et al (2011 and Kantzoura et al(2011), performed the investigations not with commonly used nad1 and cox1 genes from respectively second and third "plastic" regions of mtDNA ( Figure 3) but with part from the first one which found to be most variable. Teofanova et al (2011) have defined the population structure of liver fluke in Eastern Europe and have studied population dynamics of F. hepatica and genetic diversity of its populations in the Netherlands. Kantzoura et al (2011) have established the correlation between defined South-Eastern European genetic variants and physicogeographic characteristics of those regions and prognosticated these haplogroups distribution through mathematical modelling.…”

Section: Mitochondrial Dna Regions Used For Snp Assaymentioning

confidence: 94%

“…Nevertheless more of them do not point out any particular gene to be the most suitable marker and what are the advantages or disadvantages of that gene region usage. Comparative analyse of three gene fragments with different types of inheritance has been performed only in the research of Teofanova et al (2011). It has been established that with respect to population structuring, phylogenetic and genetic diversity studies mtDNA genes are most adequate molecule markers.…”

Section: Choosing a Gene Of An Interest To Studymentioning

confidence: 99%

“…(Mas-Coma et al, 1999;WHO, 2006). Investigations for species differentiation (Marcilla et al, 2002) and genetic heterogeneity (Vara-Del Rio et al, 2007) also have been performed (Section 3.1.1) but only the study of Teofanova et al (2011) gives the opportunity for lineage definition (e.g. b105A, b105G and b105C/T) aiming determination of genetic diversity of liver fluke populations.…”

Section: S Rdna Gene Regions Analysismentioning

confidence: 99%

See 4 more Smart Citations

Issues Associated with Genetic Diversity Studies of the Liver Fluke, Fasciola Heptica (Platyhelminthes, Digenea, Fasciolidae)

Teofanova¹,

Hristov²,

Yoveva³

et al. 2012

Genetic Diversity in Microorganisms

View full text Add to dashboard Cite

Section: S Rdna Gene Regions Analysismentioning

confidence: 86%

Section: Mitochondrial Dna Regions Used For Snp Assaymentioning

confidence: 94%

Section: Mitochondrial Dna Regions Used For Snp Assaymentioning

confidence: 94%

Section: Choosing a Gene Of An Interest To Studymentioning

confidence: 99%

Section: S Rdna Gene Regions Analysismentioning

confidence: 99%

See 3 more Smart Citations

Issues Associated with Genetic Diversity Studies of the Liver Fluke, Fasciola Heptica (Platyhelminthes, Digenea, Fasciolidae)

Teofanova¹,

Hristov²,

Yoveva³

et al. 2012

Genetic Diversity in Microorganisms

View full text Add to dashboard Cite

Morphological and molecular characterization of Fasciola isolates from livestock in Golestan province, northern Iran

Sharbatkhori

Nasibi

Mohammadi

et al. 2023

Veterinary Medicine & Sci

View full text Add to dashboard Cite

Background: Fascioliasis, caused by the liver flukes Fasciola hepatica and Fasciola gigantica, is a global zoonotic helminthic disease. The livestock and human are the final hosts of the parasites. Northern Iran is an important endemic region for fascioliasis. Few studies have been conducted on the characterization of Fasciola isolates from eastern regions of the Caspian littoral of the country. Objective:The aim of the present study was to identify F. hepatica, F. gigantica and intermediate/hybrid forms of Fasciola isolates from livestock in Golestan province, northern Iran, using morphometric and molecular tools.Methods: Livestock livers naturally infected with Fasciola spp. were collected from Golestan slaughterhouse during 2019-2020. The worms were morphometrically studied using a calibrated stereomicroscope. Genomic DNA was extracted from all samples, and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed on internal transcribed spacer (ITS1) region using Rsa1 restriction enzyme. All the isolates were then analysed by multiplex PCR on Pepck region.Results: A total of 110 Fasciola isolates were collected from the infected livers, including 94 sheep, 12 cattle and 4 goats. Morphometric analysis of 61 adult Fasciola isolates indicated that, 44 and 17 isolates belonged to F. hepatica and F. gigantica, respectively.Eighty-one and 29 isolates belonged to F. hepatica and F. gigantica using ITS1-RFLP, respectively. However, Pepck Multiplex PCR indicated 72 F. hepatica, 26 F. gigantica and 12 intermediate/hybrid forms. All 12 hybrid isolates were found in sheep host. Two isolates were identified as F. gigantica using morphometry and F. hepatica using both molecular methods. Conclusion:The present study confirmed the existence of both F. hepatica and F. gigantica species and reported the first molecular evidence of hybrid Fasciola isolates in ruminants of Golestan province.

show abstract